Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil
Abstract Background Microscopic detection of malaria parasites is the standard method for clinical diagnosis of malaria in Brazil. However, malaria epidemiological surveillance studies specifically aimed at the detection of low-density infection and asymptomatic cases will require more sensitive and...
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ftdoajarticles:oai:doaj.org/article:8c91f8df44cb489a94e5a3d862674238 2023-05-15T15:17:07+02:00 Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil Heather M. Kudyba Jaime Louzada Dragan Ljolje Karl A. Kudyba Vasant Muralidharan Joseli Oliveira-Ferreira Naomi W. Lucchi 2019-03-01T00:00:00Z https://doi.org/10.1186/s12936-019-2722-1 https://doaj.org/article/8c91f8df44cb489a94e5a3d862674238 EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2722-1 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2722-1 1475-2875 https://doaj.org/article/8c91f8df44cb489a94e5a3d862674238 Malaria Journal, Vol 18, Iss 1, Pp 1-7 (2019) Malaria Plasmodium Malachite green loop-mediated isothermal amplification Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2722-1 2022-12-30T21:07:46Z Abstract Background Microscopic detection of malaria parasites is the standard method for clinical diagnosis of malaria in Brazil. However, malaria epidemiological surveillance studies specifically aimed at the detection of low-density infection and asymptomatic cases will require more sensitive and field-usable tools. The diagnostic accuracy of the colorimetric malachite green, loop-mediated, isothermal amplification (MG-LAMP) assay was evaluated in remote health posts in Roraima state, Brazil. Methods Study participants were prospectively enrolled from health posts (healthcare-seeking patients) and from nearby villages (healthy participants) in three different study sites. The MG-LAMP assay and microscopy were performed in the health posts. Two independent readers scored the MG-LAMP tests as positive (blue/green) or negative (clear). Sensitivity and specificity of local microscopy and MG-LAMP were calculated using results of PET-PCR as a reference. Results A total of 91 participants were enrolled. There was 100% agreement between the two MG-LAMP readers (Kappa = 1). The overall sensitivity and specificity of MG-LAMP were 90.0% (95% confidence interval (CI) 76.34–97.21%) and 94% (95% CI 83.76–98.77%), respectively. The sensitivity and specificity of local microscopy were 83% (95% CI 67.22–92.66%) and 100% (95% CI 93.02–100.00%), respectively. PET-PCR detected six mixed infections (infection with both Plasmodium falciparum and Plasmodium vivax); two of these were also detected by MG-LAMP and one by microscopy. Microscopy did not detect any Plasmodium infection in the 26 healthy participants; MG-LAMP detected Plasmodium in five of these and PET-PCR assay detected infection in three. Overall, performing the MG-LAMP in this setting did not present any particular challenges. Conclusion MG-LAMP is a sensitive and specific assay that may be useful for the detection of malaria parasites in remote healthcare settings. These findings suggest that it is possible to implement simple molecular tests in facilities with ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Malaria Plasmodium Malachite green loop-mediated isothermal amplification Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Plasmodium Malachite green loop-mediated isothermal amplification Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Heather M. Kudyba Jaime Louzada Dragan Ljolje Karl A. Kudyba Vasant Muralidharan Joseli Oliveira-Ferreira Naomi W. Lucchi Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil |
topic_facet |
Malaria Plasmodium Malachite green loop-mediated isothermal amplification Diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Microscopic detection of malaria parasites is the standard method for clinical diagnosis of malaria in Brazil. However, malaria epidemiological surveillance studies specifically aimed at the detection of low-density infection and asymptomatic cases will require more sensitive and field-usable tools. The diagnostic accuracy of the colorimetric malachite green, loop-mediated, isothermal amplification (MG-LAMP) assay was evaluated in remote health posts in Roraima state, Brazil. Methods Study participants were prospectively enrolled from health posts (healthcare-seeking patients) and from nearby villages (healthy participants) in three different study sites. The MG-LAMP assay and microscopy were performed in the health posts. Two independent readers scored the MG-LAMP tests as positive (blue/green) or negative (clear). Sensitivity and specificity of local microscopy and MG-LAMP were calculated using results of PET-PCR as a reference. Results A total of 91 participants were enrolled. There was 100% agreement between the two MG-LAMP readers (Kappa = 1). The overall sensitivity and specificity of MG-LAMP were 90.0% (95% confidence interval (CI) 76.34–97.21%) and 94% (95% CI 83.76–98.77%), respectively. The sensitivity and specificity of local microscopy were 83% (95% CI 67.22–92.66%) and 100% (95% CI 93.02–100.00%), respectively. PET-PCR detected six mixed infections (infection with both Plasmodium falciparum and Plasmodium vivax); two of these were also detected by MG-LAMP and one by microscopy. Microscopy did not detect any Plasmodium infection in the 26 healthy participants; MG-LAMP detected Plasmodium in five of these and PET-PCR assay detected infection in three. Overall, performing the MG-LAMP in this setting did not present any particular challenges. Conclusion MG-LAMP is a sensitive and specific assay that may be useful for the detection of malaria parasites in remote healthcare settings. These findings suggest that it is possible to implement simple molecular tests in facilities with ... |
format |
Article in Journal/Newspaper |
author |
Heather M. Kudyba Jaime Louzada Dragan Ljolje Karl A. Kudyba Vasant Muralidharan Joseli Oliveira-Ferreira Naomi W. Lucchi |
author_facet |
Heather M. Kudyba Jaime Louzada Dragan Ljolje Karl A. Kudyba Vasant Muralidharan Joseli Oliveira-Ferreira Naomi W. Lucchi |
author_sort |
Heather M. Kudyba |
title |
Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil |
title_short |
Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil |
title_full |
Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil |
title_fullStr |
Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil |
title_full_unstemmed |
Field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in Roraima state, Brazil |
title_sort |
field evaluation of malaria malachite green loop-mediated isothermal amplification in health posts in roraima state, brazil |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2722-1 https://doaj.org/article/8c91f8df44cb489a94e5a3d862674238 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-7 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2722-1 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2722-1 1475-2875 https://doaj.org/article/8c91f8df44cb489a94e5a3d862674238 |
op_doi |
https://doi.org/10.1186/s12936-019-2722-1 |
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Malaria Journal |
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18 |
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1 |
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1766347394058813440 |