Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2)
Hemocytes mediate a series of immune reactions essential for bivalve survival in the environment, however, the impact of harmful algal species and their associated phycotoxins upon bivalve immune system is under debate. To better understand the possible toxic effects of these toxins, Crassostrea gig...
Published in: | Marine Drugs |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
MDPI AG
2012
|
Subjects: | |
Online Access: | https://doi.org/10.3390/md10030583 https://doaj.org/article/8c3e2e9308db4b7f897f5c43c9726832 |
id |
ftdoajarticles:oai:doaj.org/article:8c3e2e9308db4b7f897f5c43c9726832 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:8c3e2e9308db4b7f897f5c43c9726832 2023-05-15T15:57:42+02:00 Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) Alcir Luiz Dafre Margherita Anna Barracco Rafael Trevisan Erik Simoes Renato C. Vieira Eliza S. de Oliveira Danielle F. Mello 2012-03-01T00:00:00Z https://doi.org/10.3390/md10030583 https://doaj.org/article/8c3e2e9308db4b7f897f5c43c9726832 EN eng MDPI AG http://www.mdpi.com/1660-3397/10/3/583/ https://doaj.org/toc/1660-3397 doi:10.3390/md10030583 1660-3397 https://doaj.org/article/8c3e2e9308db4b7f897f5c43c9726832 Marine Drugs, Vol 10, Iss 3, Pp 583-597 (2012) brevetoxin hemocytes bivalves gene expression immune antioxidant and detoxification systems Biology (General) QH301-705.5 article 2012 ftdoajarticles https://doi.org/10.3390/md10030583 2022-12-30T23:50:01Z Hemocytes mediate a series of immune reactions essential for bivalve survival in the environment, however, the impact of harmful algal species and their associated phycotoxins upon bivalve immune system is under debate. To better understand the possible toxic effects of these toxins, Crassostrea gigas hemocytes were exposed to brevetoxin (PbTx-2). Hemocyte viability, monitored through the neutral red retention and MTT reduction assays, and apoptosis (Hoechst staining) remained unchanged during 12 h of exposure to PbTx-2 in concentrations up to 1000 µg/L. Despite cell viability and apoptosis remained stable, hemocytes incubated for 4 h with 1000 µg/L of PbTx-2 revealed higher expression levels of Hsp70 (p < 0.01) and CYP356A1 ( p < 0.05) transcripts and a tendency to increase FABP expression, as evaluated by Real-Time quantitative PCR. The expression of other studied genes (BPI, IL-17, GSTO, EcSOD, Prx6, SOD and GPx) remained unchanged. The results suggest that the absence of cytotoxic effects of PbTx-2 in Crassostrea gigas hemocytes, even at high concentrations, allow early defense responses to be produced by activating protective mechanisms associated to detoxification (CYP356A1 and possibly FABP) and stress (Hsp70), but not to immune or to antioxidant (BPI, IL-17, EcSOD, Prx6, GPx and SOD) related genes. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Marine Drugs 10 12 583 597 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
brevetoxin hemocytes bivalves gene expression immune antioxidant and detoxification systems Biology (General) QH301-705.5 |
spellingShingle |
brevetoxin hemocytes bivalves gene expression immune antioxidant and detoxification systems Biology (General) QH301-705.5 Alcir Luiz Dafre Margherita Anna Barracco Rafael Trevisan Erik Simoes Renato C. Vieira Eliza S. de Oliveira Danielle F. Mello Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
topic_facet |
brevetoxin hemocytes bivalves gene expression immune antioxidant and detoxification systems Biology (General) QH301-705.5 |
description |
Hemocytes mediate a series of immune reactions essential for bivalve survival in the environment, however, the impact of harmful algal species and their associated phycotoxins upon bivalve immune system is under debate. To better understand the possible toxic effects of these toxins, Crassostrea gigas hemocytes were exposed to brevetoxin (PbTx-2). Hemocyte viability, monitored through the neutral red retention and MTT reduction assays, and apoptosis (Hoechst staining) remained unchanged during 12 h of exposure to PbTx-2 in concentrations up to 1000 µg/L. Despite cell viability and apoptosis remained stable, hemocytes incubated for 4 h with 1000 µg/L of PbTx-2 revealed higher expression levels of Hsp70 (p < 0.01) and CYP356A1 ( p < 0.05) transcripts and a tendency to increase FABP expression, as evaluated by Real-Time quantitative PCR. The expression of other studied genes (BPI, IL-17, GSTO, EcSOD, Prx6, SOD and GPx) remained unchanged. The results suggest that the absence of cytotoxic effects of PbTx-2 in Crassostrea gigas hemocytes, even at high concentrations, allow early defense responses to be produced by activating protective mechanisms associated to detoxification (CYP356A1 and possibly FABP) and stress (Hsp70), but not to immune or to antioxidant (BPI, IL-17, EcSOD, Prx6, GPx and SOD) related genes. |
format |
Article in Journal/Newspaper |
author |
Alcir Luiz Dafre Margherita Anna Barracco Rafael Trevisan Erik Simoes Renato C. Vieira Eliza S. de Oliveira Danielle F. Mello |
author_facet |
Alcir Luiz Dafre Margherita Anna Barracco Rafael Trevisan Erik Simoes Renato C. Vieira Eliza S. de Oliveira Danielle F. Mello |
author_sort |
Alcir Luiz Dafre |
title |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_short |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_full |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_fullStr |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_full_unstemmed |
Cellular and Transcriptional Responses of Crassostrea gigas Hemocytes Exposed in Vitro to Brevetoxin (PbTx-2) |
title_sort |
cellular and transcriptional responses of crassostrea gigas hemocytes exposed in vitro to brevetoxin (pbtx-2) |
publisher |
MDPI AG |
publishDate |
2012 |
url |
https://doi.org/10.3390/md10030583 https://doaj.org/article/8c3e2e9308db4b7f897f5c43c9726832 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Marine Drugs, Vol 10, Iss 3, Pp 583-597 (2012) |
op_relation |
http://www.mdpi.com/1660-3397/10/3/583/ https://doaj.org/toc/1660-3397 doi:10.3390/md10030583 1660-3397 https://doaj.org/article/8c3e2e9308db4b7f897f5c43c9726832 |
op_doi |
https://doi.org/10.3390/md10030583 |
container_title |
Marine Drugs |
container_volume |
10 |
container_issue |
12 |
container_start_page |
583 |
op_container_end_page |
597 |
_version_ |
1766393385848930304 |