Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge
Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteoly...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
SciELO
2009
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S1678-91992009000200011 https://doaj.org/article/8c18e9942c8941cb9505f79ebe8bf420 |
id |
ftdoajarticles:oai:doaj.org/article:8c18e9942c8941cb9505f79ebe8bf420 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:8c18e9942c8941cb9505f79ebe8bf420 2023-05-15T15:05:06+02:00 Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge PA Miyasato CRR Ramos PAE Abreu WO Dias C Nascimento PL Ho T Kawano 2009-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992009000200011 https://doaj.org/article/8c18e9942c8941cb9505f79ebe8bf420 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000200011 1678-9199 https://doaj.org/article/8c18e9942c8941cb9505f79ebe8bf420 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 2, Pp 289-304 (2009) Schistosoma mansoni cysteine proteinase cathepsin L1 vaccine immunogenicity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2009 ftdoajarticles https://doi.org/10.1590/S1678-91992009000200011 2022-12-31T01:07:56Z Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing conditions through nickel-charged chromatography and used for experimental animal vaccination. ELISA was performed with the pooled sera. Although this protein was shown to be immunogenic, mice immunized with three doses of recombinant protein plus aluminum hydroxide as adjuvant were not protected against S. mansoni infection. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 15 2 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Schistosoma mansoni cysteine proteinase cathepsin L1 vaccine immunogenicity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Schistosoma mansoni cysteine proteinase cathepsin L1 vaccine immunogenicity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 PA Miyasato CRR Ramos PAE Abreu WO Dias C Nascimento PL Ho T Kawano Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
topic_facet |
Schistosoma mansoni cysteine proteinase cathepsin L1 vaccine immunogenicity Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Schistosomes utilize proteinases to accomplish several activities such as tissue penetration, tissue digestion and evasion of host immune responses. Cathepsin L is a cysteine proteinase of the papain superfamily detected in their gut lumen which indicates that this enzyme contributes to the proteolysis of ingested hemoglobin. Due to the roles played in the schistosome biology, proteolytic enzymes are considered potential targets for developing and guiding antischistosomal therapies. In the present work, the cathepsin L1 cDNA coding of Schistosoma mansoni was cloned into the pAE vector that provides high-level expression of heterologous proteins in Escherichia coli. The recombinant protein was expressed as inclusion bodies, purified under denaturing conditions through nickel-charged chromatography and used for experimental animal vaccination. ELISA was performed with the pooled sera. Although this protein was shown to be immunogenic, mice immunized with three doses of recombinant protein plus aluminum hydroxide as adjuvant were not protected against S. mansoni infection. |
format |
Article in Journal/Newspaper |
author |
PA Miyasato CRR Ramos PAE Abreu WO Dias C Nascimento PL Ho T Kawano |
author_facet |
PA Miyasato CRR Ramos PAE Abreu WO Dias C Nascimento PL Ho T Kawano |
author_sort |
PA Miyasato |
title |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title_short |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title_full |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title_fullStr |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title_full_unstemmed |
Expression of schistosomal cathepsin l1 in Escherichia coli and evaluation of its protective capacity in an animal challenge |
title_sort |
expression of schistosomal cathepsin l1 in escherichia coli and evaluation of its protective capacity in an animal challenge |
publisher |
SciELO |
publishDate |
2009 |
url |
https://doi.org/10.1590/S1678-91992009000200011 https://doaj.org/article/8c18e9942c8941cb9505f79ebe8bf420 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 15, Iss 2, Pp 289-304 (2009) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200011 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992009000200011 1678-9199 https://doaj.org/article/8c18e9942c8941cb9505f79ebe8bf420 |
op_doi |
https://doi.org/10.1590/S1678-91992009000200011 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
15 |
container_issue |
2 |
_version_ |
1766336851932610560 |