Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field.
A novel GH9 cellulase (AMOR_GH9A) was discovered by sequence-based mining of a unique metagenomic dataset collected at the Jan Mayen hydrothermal vent field. AMOR_GH9A comprises a signal peptide, a catalytic domain and a CBM3 cellulose-binding module. AMOR_GH9A is an exceptionally stable enzyme with...
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ftdoajarticles:oai:doaj.org/article:8c07677ca1f6436582009622431a3f7b 2023-05-15T15:02:24+02:00 Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. Anton A Stepnov Lasse Fredriksen Ida H Steen Runar Stokke Vincent G H Eijsink 2019-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0222216 https://doaj.org/article/8c07677ca1f6436582009622431a3f7b EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pone.0222216 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0222216 https://doaj.org/article/8c07677ca1f6436582009622431a3f7b PLoS ONE, Vol 14, Iss 9, p e0222216 (2019) Medicine R Science Q article 2019 ftdoajarticles https://doi.org/10.1371/journal.pone.0222216 2022-12-31T13:15:07Z A novel GH9 cellulase (AMOR_GH9A) was discovered by sequence-based mining of a unique metagenomic dataset collected at the Jan Mayen hydrothermal vent field. AMOR_GH9A comprises a signal peptide, a catalytic domain and a CBM3 cellulose-binding module. AMOR_GH9A is an exceptionally stable enzyme with a temperature optimum around 100°C and an apparent melting temperature of 105°C. The novel cellulase retains 64% of its activity after 4 hours of incubation at 95°C. The closest characterized homolog of AMOR_GH9A is TfCel9A, a processive endocellulase from the model thermophilic bacterium Thermobifida fusca (64.2% sequence identity). Direct comparison of AMOR_GH9A and TfCel9A revealed that AMOR_GH9A possesses higher activity on soluble and amorphous substrates (phosphoric acid swollen cellulose, konjac glucomannan) and has an ability to hydrolyse xylan that is lacking in TfCel9A. Article in Journal/Newspaper Arctic Jan Mayen Directory of Open Access Journals: DOAJ Articles Arctic Jan Mayen PLOS ONE 14 9 e0222216 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
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Medicine R Science Q Anton A Stepnov Lasse Fredriksen Ida H Steen Runar Stokke Vincent G H Eijsink Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. |
topic_facet |
Medicine R Science Q |
description |
A novel GH9 cellulase (AMOR_GH9A) was discovered by sequence-based mining of a unique metagenomic dataset collected at the Jan Mayen hydrothermal vent field. AMOR_GH9A comprises a signal peptide, a catalytic domain and a CBM3 cellulose-binding module. AMOR_GH9A is an exceptionally stable enzyme with a temperature optimum around 100°C and an apparent melting temperature of 105°C. The novel cellulase retains 64% of its activity after 4 hours of incubation at 95°C. The closest characterized homolog of AMOR_GH9A is TfCel9A, a processive endocellulase from the model thermophilic bacterium Thermobifida fusca (64.2% sequence identity). Direct comparison of AMOR_GH9A and TfCel9A revealed that AMOR_GH9A possesses higher activity on soluble and amorphous substrates (phosphoric acid swollen cellulose, konjac glucomannan) and has an ability to hydrolyse xylan that is lacking in TfCel9A. |
format |
Article in Journal/Newspaper |
author |
Anton A Stepnov Lasse Fredriksen Ida H Steen Runar Stokke Vincent G H Eijsink |
author_facet |
Anton A Stepnov Lasse Fredriksen Ida H Steen Runar Stokke Vincent G H Eijsink |
author_sort |
Anton A Stepnov |
title |
Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. |
title_short |
Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. |
title_full |
Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. |
title_fullStr |
Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. |
title_full_unstemmed |
Identification and characterization of a hyperthermophilic GH9 cellulase from the Arctic Mid-Ocean Ridge vent field. |
title_sort |
identification and characterization of a hyperthermophilic gh9 cellulase from the arctic mid-ocean ridge vent field. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pone.0222216 https://doaj.org/article/8c07677ca1f6436582009622431a3f7b |
geographic |
Arctic Jan Mayen |
geographic_facet |
Arctic Jan Mayen |
genre |
Arctic Jan Mayen |
genre_facet |
Arctic Jan Mayen |
op_source |
PLoS ONE, Vol 14, Iss 9, p e0222216 (2019) |
op_relation |
https://doi.org/10.1371/journal.pone.0222216 https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0222216 https://doaj.org/article/8c07677ca1f6436582009622431a3f7b |
op_doi |
https://doi.org/10.1371/journal.pone.0222216 |
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PLOS ONE |
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14 |
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9 |
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e0222216 |
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