un Identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to RT-PCR
Introduction: Enteric viruses have been associated with the production of a variety of diseases transmitted by the fecal-oral route and carried through contaminated food and water. Given their structure and composition, they are highly resistant to environmental conditions and most of the chemical a...
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Instituto Nacional de Salud
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ftdoajarticles:oai:doaj.org/article:8ba604b051694f9c93430ed0ad1953e2 2023-05-15T15:09:58+02:00 un Identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to RT-PCR Raquel Villamizar Dioselina Peláez-Carvajal Luis Felipe Acero 2021-12-01T00:00:00Z https://doi.org/10.7705/biomedica.6032 https://doaj.org/article/8ba604b051694f9c93430ed0ad1953e2 EN ES eng spa Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/6032 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.6032 https://doaj.org/article/8ba604b051694f9c93430ed0ad1953e2 Biomédica: revista del Instituto Nacional de Salud, Vol 41, Iss 4, Pp 745-755 (2021) rotavirus infections hepatitis a antibodies raw water water purification magnetic segregation Medicine R Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.7705/biomedica.6032 2022-12-31T02:11:10Z Introduction: Enteric viruses have been associated with the production of a variety of diseases transmitted by the fecal-oral route and carried through contaminated food and water. Given their structure and composition, they are highly resistant to environmental conditions and most of the chemical agents used in the purification processes. Therefore, the systematic monitoring of raw water is necessary to ensure its quality especially when it is used for producing drinking water for human consumption. Objective: We identified the presence of rotavirus and hepatitis A virus by means of the fluoro-immuno-magnetic separation technique (FIMS) in raw water taken from four purification plants and their water supplies in the department of Norte de Santander. Materials and methods: The viruses were captured and separated from the water samples using magnetic microparticles functionalized with monoclonal anti-Hepatitis A and anti-Rotavirus antibodies. Confocal microscopy was used to monitor the viral concentration process and transmission electron microscopy for the morphological visualization of the separated viruses. The reverse transcriptase-coupled polymerase chain reaction (RT-PCR) was applied to confirm the presence of pathogens. Results: The two enteric viruses were identified in the majority of the analyzed water samples including water supply sources. Conclusion: We determined that the FIMS technique together with RT-PCR is highly effective for the detection of viral pathogens in complex matrices such as raw water. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 41 4 745 755 |
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Directory of Open Access Journals: DOAJ Articles |
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English Spanish |
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rotavirus infections hepatitis a antibodies raw water water purification magnetic segregation Medicine R Arctic medicine. Tropical medicine RC955-962 |
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rotavirus infections hepatitis a antibodies raw water water purification magnetic segregation Medicine R Arctic medicine. Tropical medicine RC955-962 Raquel Villamizar Dioselina Peláez-Carvajal Luis Felipe Acero un Identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to RT-PCR |
topic_facet |
rotavirus infections hepatitis a antibodies raw water water purification magnetic segregation Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Enteric viruses have been associated with the production of a variety of diseases transmitted by the fecal-oral route and carried through contaminated food and water. Given their structure and composition, they are highly resistant to environmental conditions and most of the chemical agents used in the purification processes. Therefore, the systematic monitoring of raw water is necessary to ensure its quality especially when it is used for producing drinking water for human consumption. Objective: We identified the presence of rotavirus and hepatitis A virus by means of the fluoro-immuno-magnetic separation technique (FIMS) in raw water taken from four purification plants and their water supplies in the department of Norte de Santander. Materials and methods: The viruses were captured and separated from the water samples using magnetic microparticles functionalized with monoclonal anti-Hepatitis A and anti-Rotavirus antibodies. Confocal microscopy was used to monitor the viral concentration process and transmission electron microscopy for the morphological visualization of the separated viruses. The reverse transcriptase-coupled polymerase chain reaction (RT-PCR) was applied to confirm the presence of pathogens. Results: The two enteric viruses were identified in the majority of the analyzed water samples including water supply sources. Conclusion: We determined that the FIMS technique together with RT-PCR is highly effective for the detection of viral pathogens in complex matrices such as raw water. |
format |
Article in Journal/Newspaper |
author |
Raquel Villamizar Dioselina Peláez-Carvajal Luis Felipe Acero |
author_facet |
Raquel Villamizar Dioselina Peláez-Carvajal Luis Felipe Acero |
author_sort |
Raquel Villamizar |
title |
un Identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to RT-PCR |
title_short |
un Identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to RT-PCR |
title_full |
un Identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to RT-PCR |
title_fullStr |
un Identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to RT-PCR |
title_full_unstemmed |
un Identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to RT-PCR |
title_sort |
un identification of enteric viruses from raw water using fluoro-immuno-magnetic separation coupled to rt-pcr |
publisher |
Instituto Nacional de Salud |
publishDate |
2021 |
url |
https://doi.org/10.7705/biomedica.6032 https://doaj.org/article/8ba604b051694f9c93430ed0ad1953e2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 41, Iss 4, Pp 745-755 (2021) |
op_relation |
https://revistabiomedica.org/index.php/biomedica/article/view/6032 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.6032 https://doaj.org/article/8ba604b051694f9c93430ed0ad1953e2 |
op_doi |
https://doi.org/10.7705/biomedica.6032 |
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Biomédica |
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41 |
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4 |
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745 |
op_container_end_page |
755 |
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