A set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses
Abstract Background Four Plasmodium falciparum genetic crosses (HB3×3D7, HB3×Dd2, 7G8×GB4, and 803×GB4) have produced sets of recombinant progeny that are widely used for malaria research, including investigations of anti-malarial drug resistance. It is critical to maintain the progeny free from cro...
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ftdoajarticles:oai:doaj.org/article:8ba0a16eb611405bbeb3e2e1870d26f6 2023-05-15T15:11:12+02:00 A set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses Christine E. Figan Juliana M. Sá Jianbing Mu Viviana A. Melendez-Muniz Chia Hao Liu Thomas E. Wellems 2018-02-01T00:00:00Z https://doi.org/10.1186/s12936-018-2210-z https://doaj.org/article/8ba0a16eb611405bbeb3e2e1870d26f6 EN eng BMC http://link.springer.com/article/10.1186/s12936-018-2210-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2210-z 1475-2875 https://doaj.org/article/8ba0a16eb611405bbeb3e2e1870d26f6 Malaria Journal, Vol 17, Iss 1, Pp 1-6 (2018) Plasmodium falciparum Genotyping Molecular fingerprint Parasite clones Cross-contamination Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2018 ftdoajarticles https://doi.org/10.1186/s12936-018-2210-z 2022-12-31T03:44:06Z Abstract Background Four Plasmodium falciparum genetic crosses (HB3×3D7, HB3×Dd2, 7G8×GB4, and 803×GB4) have produced sets of recombinant progeny that are widely used for malaria research, including investigations of anti-malarial drug resistance. It is critical to maintain the progeny free from cross-contamination. Microsatellite polymorphisms can be used to validate parasite identity. Results A set of 12 markers was developed that differentiates the parents of the four P. falciparum crosses. This typing set identified distinguishing patterns of inheritance (fingerprints) in segregant collections of 15 progeny clones from HB3×3D7, 32 from HB3×Dd2, 33 from 7G8×GB4, and 81 from 803×GB4. Stronger amplification was observed with shorter relative to longer alleles of individual microsatellites. In experiments with mixed parental DNAs, electropherograms showed that signals of cross-contamination can be missed when minor peaks less than 1/4 or 1/3 the height of the major peak are disregarded by threshold settings commonly used for population studies. Conclusions Microsatellite typing is an effective method to check the identity of P. falciparum lines and detect parasite cross-contamination in cultures; however, care must be taken not to ignore minor peaks that can be overlooked. The 12 microsatellite markers presented here provide a rapid and efficient means to distinguish the segregants of laboratory crosses. Fingerprint patterns from these markers are useful to maintain the integrity of diverse parasite lines in and between research laboratories. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 17 1 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Plasmodium falciparum Genotyping Molecular fingerprint Parasite clones Cross-contamination Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Plasmodium falciparum Genotyping Molecular fingerprint Parasite clones Cross-contamination Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Christine E. Figan Juliana M. Sá Jianbing Mu Viviana A. Melendez-Muniz Chia Hao Liu Thomas E. Wellems A set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses |
topic_facet |
Plasmodium falciparum Genotyping Molecular fingerprint Parasite clones Cross-contamination Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Four Plasmodium falciparum genetic crosses (HB3×3D7, HB3×Dd2, 7G8×GB4, and 803×GB4) have produced sets of recombinant progeny that are widely used for malaria research, including investigations of anti-malarial drug resistance. It is critical to maintain the progeny free from cross-contamination. Microsatellite polymorphisms can be used to validate parasite identity. Results A set of 12 markers was developed that differentiates the parents of the four P. falciparum crosses. This typing set identified distinguishing patterns of inheritance (fingerprints) in segregant collections of 15 progeny clones from HB3×3D7, 32 from HB3×Dd2, 33 from 7G8×GB4, and 81 from 803×GB4. Stronger amplification was observed with shorter relative to longer alleles of individual microsatellites. In experiments with mixed parental DNAs, electropherograms showed that signals of cross-contamination can be missed when minor peaks less than 1/4 or 1/3 the height of the major peak are disregarded by threshold settings commonly used for population studies. Conclusions Microsatellite typing is an effective method to check the identity of P. falciparum lines and detect parasite cross-contamination in cultures; however, care must be taken not to ignore minor peaks that can be overlooked. The 12 microsatellite markers presented here provide a rapid and efficient means to distinguish the segregants of laboratory crosses. Fingerprint patterns from these markers are useful to maintain the integrity of diverse parasite lines in and between research laboratories. |
format |
Article in Journal/Newspaper |
author |
Christine E. Figan Juliana M. Sá Jianbing Mu Viviana A. Melendez-Muniz Chia Hao Liu Thomas E. Wellems |
author_facet |
Christine E. Figan Juliana M. Sá Jianbing Mu Viviana A. Melendez-Muniz Chia Hao Liu Thomas E. Wellems |
author_sort |
Christine E. Figan |
title |
A set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses |
title_short |
A set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses |
title_full |
A set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses |
title_fullStr |
A set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses |
title_full_unstemmed |
A set of microsatellite markers to differentiate Plasmodium falciparum progeny of four genetic crosses |
title_sort |
set of microsatellite markers to differentiate plasmodium falciparum progeny of four genetic crosses |
publisher |
BMC |
publishDate |
2018 |
url |
https://doi.org/10.1186/s12936-018-2210-z https://doaj.org/article/8ba0a16eb611405bbeb3e2e1870d26f6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 17, Iss 1, Pp 1-6 (2018) |
op_relation |
http://link.springer.com/article/10.1186/s12936-018-2210-z https://doaj.org/toc/1475-2875 doi:10.1186/s12936-018-2210-z 1475-2875 https://doaj.org/article/8ba0a16eb611405bbeb3e2e1870d26f6 |
op_doi |
https://doi.org/10.1186/s12936-018-2210-z |
container_title |
Malaria Journal |
container_volume |
17 |
container_issue |
1 |
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1766342101122940928 |