The detection of pfcrt and pfmdr1 point mutations as molecular markers of chloroquine drug resistance, Pahang, Malaysia
Abstract Background Malaria is still a public health problem in Malaysia with chloroquine (CQ) being the first-line drug in the treatment policy of uncomplicated malaria. There is a scarcity in information about the magnitude of Plasmodium falciparum CQ resistance. This study aims to investigate the...
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ftdoajarticles:oai:doaj.org/article:8b996e3e31d245cdb61b85e25f14b21d 2023-05-15T15:13:26+02:00 The detection of pfcrt and pfmdr1 point mutations as molecular markers of chloroquine drug resistance, Pahang, Malaysia Atroosh Wahib M Al-Mekhlafi Hesham M Mahdy Mohammed AK Surin Johari 2012-08-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-251 https://doaj.org/article/8b996e3e31d245cdb61b85e25f14b21d EN eng BMC http://www.malariajournal.com/content/11/1/251 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-251 1475-2875 https://doaj.org/article/8b996e3e31d245cdb61b85e25f14b21d Malaria Journal, Vol 11, Iss 1, p 251 (2012) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-251 2022-12-30T22:46:02Z Abstract Background Malaria is still a public health problem in Malaysia with chloroquine (CQ) being the first-line drug in the treatment policy of uncomplicated malaria. There is a scarcity in information about the magnitude of Plasmodium falciparum CQ resistance. This study aims to investigate the presence of single point mutations in the P. falciparum chloroquine-resistance transporter gene ( pfcrt ) at codons 76, 271, 326, 356 and 371 and in P. falciparum multi-drug resistance-1 gene ( pfmdr1 ) at codons 86 and 1246, as molecular markers of CQ resistance. Methods A total of 75 P. falciparum blood samples were collected from different districts of Pahang state, Malaysia. Single nucleotide polymorphisms in pfcrt gene (codons 76, 271, 326, 356 and 371) and pfmdr1 gene (codons 86 and 1246) were analysed by using mutation-specific nested PCR and restriction fragment length polymorphism (PCR-RFLP) methods. Results Mutations of pfcrt K76T and pfcrt R371I were the most prevalent among pfcrt gene mutations reported by this study; 52% and 77%, respectively. Other codons of the pfcrt gene and the positions 86 and 1246 of the pfmdr1 gene were found mostly of wild type. Significant associations of pfcrt K76T, pfcrt N326S and pfcrt I356T mutations with parasitaemia were also reported. Conclusion The high existence of mutant pfcrt T76 may indicate the low susceptibility of P. falciparum isolates to CQ in Peninsular Malaysia. The findings of this study establish baseline data on the molecular markers of P. falciparum CQ resistance, which may help in the surveillance of drug resistance in Peninsular Malaysia. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 251 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Atroosh Wahib M Al-Mekhlafi Hesham M Mahdy Mohammed AK Surin Johari The detection of pfcrt and pfmdr1 point mutations as molecular markers of chloroquine drug resistance, Pahang, Malaysia |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is still a public health problem in Malaysia with chloroquine (CQ) being the first-line drug in the treatment policy of uncomplicated malaria. There is a scarcity in information about the magnitude of Plasmodium falciparum CQ resistance. This study aims to investigate the presence of single point mutations in the P. falciparum chloroquine-resistance transporter gene ( pfcrt ) at codons 76, 271, 326, 356 and 371 and in P. falciparum multi-drug resistance-1 gene ( pfmdr1 ) at codons 86 and 1246, as molecular markers of CQ resistance. Methods A total of 75 P. falciparum blood samples were collected from different districts of Pahang state, Malaysia. Single nucleotide polymorphisms in pfcrt gene (codons 76, 271, 326, 356 and 371) and pfmdr1 gene (codons 86 and 1246) were analysed by using mutation-specific nested PCR and restriction fragment length polymorphism (PCR-RFLP) methods. Results Mutations of pfcrt K76T and pfcrt R371I were the most prevalent among pfcrt gene mutations reported by this study; 52% and 77%, respectively. Other codons of the pfcrt gene and the positions 86 and 1246 of the pfmdr1 gene were found mostly of wild type. Significant associations of pfcrt K76T, pfcrt N326S and pfcrt I356T mutations with parasitaemia were also reported. Conclusion The high existence of mutant pfcrt T76 may indicate the low susceptibility of P. falciparum isolates to CQ in Peninsular Malaysia. The findings of this study establish baseline data on the molecular markers of P. falciparum CQ resistance, which may help in the surveillance of drug resistance in Peninsular Malaysia. |
format |
Article in Journal/Newspaper |
author |
Atroosh Wahib M Al-Mekhlafi Hesham M Mahdy Mohammed AK Surin Johari |
author_facet |
Atroosh Wahib M Al-Mekhlafi Hesham M Mahdy Mohammed AK Surin Johari |
author_sort |
Atroosh Wahib M |
title |
The detection of pfcrt and pfmdr1 point mutations as molecular markers of chloroquine drug resistance, Pahang, Malaysia |
title_short |
The detection of pfcrt and pfmdr1 point mutations as molecular markers of chloroquine drug resistance, Pahang, Malaysia |
title_full |
The detection of pfcrt and pfmdr1 point mutations as molecular markers of chloroquine drug resistance, Pahang, Malaysia |
title_fullStr |
The detection of pfcrt and pfmdr1 point mutations as molecular markers of chloroquine drug resistance, Pahang, Malaysia |
title_full_unstemmed |
The detection of pfcrt and pfmdr1 point mutations as molecular markers of chloroquine drug resistance, Pahang, Malaysia |
title_sort |
detection of pfcrt and pfmdr1 point mutations as molecular markers of chloroquine drug resistance, pahang, malaysia |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-251 https://doaj.org/article/8b996e3e31d245cdb61b85e25f14b21d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 251 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/251 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-251 1475-2875 https://doaj.org/article/8b996e3e31d245cdb61b85e25f14b21d |
op_doi |
https://doi.org/10.1186/1475-2875-11-251 |
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Malaria Journal |
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11 |
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1 |
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251 |
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1766343988810350592 |