LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis.
Neglected tropical diseases, including zoonoses such as leptospirosis, have a major impact on rural and poor urban communities, particularly in developing countries. This has led to major investment in antipoverty vaccines that focus on diseases that influence public health and thereby productivity....
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ftdoajarticles:oai:doaj.org/article:8b7a1455c19842dfbe3e357bb08f0ac4 2023-05-15T15:14:10+02:00 LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. Neida L Conrad Flávia W Cruz McBride Jéssica D Souza Marcelle M Silveira Samuel Félix Karla S Mendonça Cleiton S Santos Daniel A Athanazio Marco A Medeiros Mitermayer G Reis Odir A Dellagostin Alan J A McBride 2017-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005441 https://doaj.org/article/8b7a1455c19842dfbe3e357bb08f0ac4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5370146?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005441 https://doaj.org/article/8b7a1455c19842dfbe3e357bb08f0ac4 PLoS Neglected Tropical Diseases, Vol 11, Iss 3, p e0005441 (2017) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2017 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005441 2022-12-31T14:10:45Z Neglected tropical diseases, including zoonoses such as leptospirosis, have a major impact on rural and poor urban communities, particularly in developing countries. This has led to major investment in antipoverty vaccines that focus on diseases that influence public health and thereby productivity. While the true, global, impact of leptospirosis is unknown due to the lack of adequate laboratory diagnosis, the WHO estimates that incidence has doubled over the last 15 years to over 1 million cases that require hospitalization every year. Leptospirosis is caused by pathogenic Leptospira spp. and is spread through direct contact with infected animals, their urine or contaminated water and soil. Inactivated leptospirosis vaccines, or bacterins, are approved in only a handful of countries due to the lack of heterologous protection (there are > 250 pathogenic Leptospira serovars) and the serious side-effects associated with vaccination. Currently, research has focused on recombinant vaccines, a possible solution to these problems. However, due to a lack of standardised animal models, rigorous statistical analysis and poor reproducibility, this approach has met with limited success. We evaluated a subunit vaccine preparation, based on a conserved region of the leptospiral immunoglobulin-like B protein (LigB(131-645)) and aluminium hydroxide (AH), in the hamster model of leptospirosis. The vaccine conferred significant protection (80.0-100%, P < 0.05) against mortality in vaccinated animals in seven independent experiments. The efficacy of the LigB(131-645)/AH vaccine ranged from 87.5-100% and we observed sterile immunity (87.5-100%) among the vaccinated survivors. Significant levels of IgM and IgG were induced among vaccinated animals, although they did not correlate with immunity. A mixed IgG1/IgG2 subclass profile was associated with the subunit vaccine, compared to the predominant IgG2 profile seen in bacterin vaccinated hamsters. These findings suggest that LigB(131-645) is a vaccine candidate against ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 11 3 e0005441 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Neida L Conrad Flávia W Cruz McBride Jéssica D Souza Marcelle M Silveira Samuel Félix Karla S Mendonça Cleiton S Santos Daniel A Athanazio Marco A Medeiros Mitermayer G Reis Odir A Dellagostin Alan J A McBride LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Neglected tropical diseases, including zoonoses such as leptospirosis, have a major impact on rural and poor urban communities, particularly in developing countries. This has led to major investment in antipoverty vaccines that focus on diseases that influence public health and thereby productivity. While the true, global, impact of leptospirosis is unknown due to the lack of adequate laboratory diagnosis, the WHO estimates that incidence has doubled over the last 15 years to over 1 million cases that require hospitalization every year. Leptospirosis is caused by pathogenic Leptospira spp. and is spread through direct contact with infected animals, their urine or contaminated water and soil. Inactivated leptospirosis vaccines, or bacterins, are approved in only a handful of countries due to the lack of heterologous protection (there are > 250 pathogenic Leptospira serovars) and the serious side-effects associated with vaccination. Currently, research has focused on recombinant vaccines, a possible solution to these problems. However, due to a lack of standardised animal models, rigorous statistical analysis and poor reproducibility, this approach has met with limited success. We evaluated a subunit vaccine preparation, based on a conserved region of the leptospiral immunoglobulin-like B protein (LigB(131-645)) and aluminium hydroxide (AH), in the hamster model of leptospirosis. The vaccine conferred significant protection (80.0-100%, P < 0.05) against mortality in vaccinated animals in seven independent experiments. The efficacy of the LigB(131-645)/AH vaccine ranged from 87.5-100% and we observed sterile immunity (87.5-100%) among the vaccinated survivors. Significant levels of IgM and IgG were induced among vaccinated animals, although they did not correlate with immunity. A mixed IgG1/IgG2 subclass profile was associated with the subunit vaccine, compared to the predominant IgG2 profile seen in bacterin vaccinated hamsters. These findings suggest that LigB(131-645) is a vaccine candidate against ... |
format |
Article in Journal/Newspaper |
author |
Neida L Conrad Flávia W Cruz McBride Jéssica D Souza Marcelle M Silveira Samuel Félix Karla S Mendonça Cleiton S Santos Daniel A Athanazio Marco A Medeiros Mitermayer G Reis Odir A Dellagostin Alan J A McBride |
author_facet |
Neida L Conrad Flávia W Cruz McBride Jéssica D Souza Marcelle M Silveira Samuel Félix Karla S Mendonça Cleiton S Santos Daniel A Athanazio Marco A Medeiros Mitermayer G Reis Odir A Dellagostin Alan J A McBride |
author_sort |
Neida L Conrad |
title |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. |
title_short |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. |
title_full |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. |
title_fullStr |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. |
title_full_unstemmed |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. |
title_sort |
ligb subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2017 |
url |
https://doi.org/10.1371/journal.pntd.0005441 https://doaj.org/article/8b7a1455c19842dfbe3e357bb08f0ac4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 11, Iss 3, p e0005441 (2017) |
op_relation |
http://europepmc.org/articles/PMC5370146?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005441 https://doaj.org/article/8b7a1455c19842dfbe3e357bb08f0ac4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005441 |
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PLOS Neglected Tropical Diseases |
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11 |
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3 |
container_start_page |
e0005441 |
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