Transient Receptor Potential-Vanilloid (TRPV1-TRPV4) Channels in the Atlantic Salmon, Salmo salar. A Focus on the Pineal Gland and Melatonin Production
Fish are ectotherm, which rely on the external temperature to regulate their internal body temperature, although some may perform partial endothermy. Together with photoperiod, temperature oscillations, contribute to synchronizing the daily and seasonal variations of fish metabolism, physiology and...
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2022
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ftdoajarticles:oai:doaj.org/article:8b56710fedb14144a130d9079235eafd 2023-05-15T15:31:13+02:00 Transient Receptor Potential-Vanilloid (TRPV1-TRPV4) Channels in the Atlantic Salmon, Salmo salar. A Focus on the Pineal Gland and Melatonin Production Laura Gabriela Nisembaum Guillaume Loentgen Thibaut L’Honoré Patrick Martin Charles-Hubert Paulin Michael Fuentès Karine Escoubeyrou María Jesús Delgado Laurence Besseau Jack Falcón 2022-01-01T00:00:00Z https://doi.org/10.3389/fphys.2021.784416 https://doaj.org/article/8b56710fedb14144a130d9079235eafd EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fphys.2021.784416/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2021.784416 https://doaj.org/article/8b56710fedb14144a130d9079235eafd Frontiers in Physiology, Vol 12 (2022) Atlantic salmon temperature pineal organ melatonin transient receptor potential vanilloid (TRPV) TRPV1 Physiology QP1-981 article 2022 ftdoajarticles https://doi.org/10.3389/fphys.2021.784416 2022-12-31T16:30:59Z Fish are ectotherm, which rely on the external temperature to regulate their internal body temperature, although some may perform partial endothermy. Together with photoperiod, temperature oscillations, contribute to synchronizing the daily and seasonal variations of fish metabolism, physiology and behavior. Recent studies are shedding light on the mechanisms of temperature sensing and behavioral thermoregulation in fish. In particular, the role of some members of the transient receptor potential channels (TRP) is being gradually unraveled. The present study in the migratory Atlantic salmon, Salmo salar, aims at identifying the tissue distribution and abundance in mRNA corresponding to the TRP of the vanilloid subfamilies, TRPV1 and TRPV4, and at characterizing their putative role in the control of the temperature-dependent modulation of melatonin production—the time-keeping hormone—by the pineal gland. In Salmo salar, TRPV1 and TRPV4 mRNA tissue distribution appeared ubiquitous; mRNA abundance varied as a function of the month investigated. In situ hybridization and immunohistochemistry indicated specific labeling located in the photoreceptor cells of the pineal gland and the retina. Additionally, TRPV analogs modulated the production of melatonin by isolated pineal glands in culture. The TRPV1 agonist induced an inhibitory response at high concentrations, while evoking a bell-shaped response (stimulatory at low, and inhibitory at high, concentrations) when added with an antagonist. The TRPV4 agonist was stimulatory at the highest concentration used. Altogether, the present results agree with the known widespread distribution and role of TRPV1 and TRPV4 channels, and with published data on trout (Oncorhynchus mykiss), leading to suggest these channels mediate the effects of temperature on S. salar pineal melatonin production. We discuss their involvement in controlling the timing of daily and seasonal events in this migratory species, in the context of an increasing warming of water temperatures. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Frontiers in Physiology 12 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic salmon temperature pineal organ melatonin transient receptor potential vanilloid (TRPV) TRPV1 Physiology QP1-981 |
spellingShingle |
Atlantic salmon temperature pineal organ melatonin transient receptor potential vanilloid (TRPV) TRPV1 Physiology QP1-981 Laura Gabriela Nisembaum Guillaume Loentgen Thibaut L’Honoré Patrick Martin Charles-Hubert Paulin Michael Fuentès Karine Escoubeyrou María Jesús Delgado Laurence Besseau Jack Falcón Transient Receptor Potential-Vanilloid (TRPV1-TRPV4) Channels in the Atlantic Salmon, Salmo salar. A Focus on the Pineal Gland and Melatonin Production |
topic_facet |
Atlantic salmon temperature pineal organ melatonin transient receptor potential vanilloid (TRPV) TRPV1 Physiology QP1-981 |
description |
Fish are ectotherm, which rely on the external temperature to regulate their internal body temperature, although some may perform partial endothermy. Together with photoperiod, temperature oscillations, contribute to synchronizing the daily and seasonal variations of fish metabolism, physiology and behavior. Recent studies are shedding light on the mechanisms of temperature sensing and behavioral thermoregulation in fish. In particular, the role of some members of the transient receptor potential channels (TRP) is being gradually unraveled. The present study in the migratory Atlantic salmon, Salmo salar, aims at identifying the tissue distribution and abundance in mRNA corresponding to the TRP of the vanilloid subfamilies, TRPV1 and TRPV4, and at characterizing their putative role in the control of the temperature-dependent modulation of melatonin production—the time-keeping hormone—by the pineal gland. In Salmo salar, TRPV1 and TRPV4 mRNA tissue distribution appeared ubiquitous; mRNA abundance varied as a function of the month investigated. In situ hybridization and immunohistochemistry indicated specific labeling located in the photoreceptor cells of the pineal gland and the retina. Additionally, TRPV analogs modulated the production of melatonin by isolated pineal glands in culture. The TRPV1 agonist induced an inhibitory response at high concentrations, while evoking a bell-shaped response (stimulatory at low, and inhibitory at high, concentrations) when added with an antagonist. The TRPV4 agonist was stimulatory at the highest concentration used. Altogether, the present results agree with the known widespread distribution and role of TRPV1 and TRPV4 channels, and with published data on trout (Oncorhynchus mykiss), leading to suggest these channels mediate the effects of temperature on S. salar pineal melatonin production. We discuss their involvement in controlling the timing of daily and seasonal events in this migratory species, in the context of an increasing warming of water temperatures. |
format |
Article in Journal/Newspaper |
author |
Laura Gabriela Nisembaum Guillaume Loentgen Thibaut L’Honoré Patrick Martin Charles-Hubert Paulin Michael Fuentès Karine Escoubeyrou María Jesús Delgado Laurence Besseau Jack Falcón |
author_facet |
Laura Gabriela Nisembaum Guillaume Loentgen Thibaut L’Honoré Patrick Martin Charles-Hubert Paulin Michael Fuentès Karine Escoubeyrou María Jesús Delgado Laurence Besseau Jack Falcón |
author_sort |
Laura Gabriela Nisembaum |
title |
Transient Receptor Potential-Vanilloid (TRPV1-TRPV4) Channels in the Atlantic Salmon, Salmo salar. A Focus on the Pineal Gland and Melatonin Production |
title_short |
Transient Receptor Potential-Vanilloid (TRPV1-TRPV4) Channels in the Atlantic Salmon, Salmo salar. A Focus on the Pineal Gland and Melatonin Production |
title_full |
Transient Receptor Potential-Vanilloid (TRPV1-TRPV4) Channels in the Atlantic Salmon, Salmo salar. A Focus on the Pineal Gland and Melatonin Production |
title_fullStr |
Transient Receptor Potential-Vanilloid (TRPV1-TRPV4) Channels in the Atlantic Salmon, Salmo salar. A Focus on the Pineal Gland and Melatonin Production |
title_full_unstemmed |
Transient Receptor Potential-Vanilloid (TRPV1-TRPV4) Channels in the Atlantic Salmon, Salmo salar. A Focus on the Pineal Gland and Melatonin Production |
title_sort |
transient receptor potential-vanilloid (trpv1-trpv4) channels in the atlantic salmon, salmo salar. a focus on the pineal gland and melatonin production |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fphys.2021.784416 https://doaj.org/article/8b56710fedb14144a130d9079235eafd |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Frontiers in Physiology, Vol 12 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fphys.2021.784416/full https://doaj.org/toc/1664-042X 1664-042X doi:10.3389/fphys.2021.784416 https://doaj.org/article/8b56710fedb14144a130d9079235eafd |
op_doi |
https://doi.org/10.3389/fphys.2021.784416 |
container_title |
Frontiers in Physiology |
container_volume |
12 |
_version_ |
1766361715082002432 |