Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia
Abstract Background 125 million women are pregnant each year in malaria endemic areas and are, therefore, at risk of Malaria in Pregnancy (MiP). MiP is the direct consequence of Plasmodium infection during pregnancy. The sequestration of Plasmodium falciparum parasites in the placenta adversely affe...
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ftdoajarticles:oai:doaj.org/article:8b4c02d9f75e47dfac01f0210e8e0309 2023-05-15T15:17:53+02:00 Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia Guluma Tadesse Claire Kamaliddin Cody Doolan Ranmalee Amarasekara Ruth Legese Abu Naser Mohon James Cheaveau Delenasaw Yewhalaw Dylan R. Pillai 2020-08-01T00:00:00Z https://doi.org/10.1186/s12936-020-03380-9 https://doaj.org/article/8b4c02d9f75e47dfac01f0210e8e0309 EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03380-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03380-9 1475-2875 https://doaj.org/article/8b4c02d9f75e47dfac01f0210e8e0309 Malaria Journal, Vol 19, Iss 1, Pp 1-9 (2020) Malaria Asymptomatic infections Pregnancy LAMP Low birth weight Anemia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03380-9 2022-12-31T08:39:22Z Abstract Background 125 million women are pregnant each year in malaria endemic areas and are, therefore, at risk of Malaria in Pregnancy (MiP). MiP is the direct consequence of Plasmodium infection during pregnancy. The sequestration of Plasmodium falciparum parasites in the placenta adversely affects fetal development and impacts newborn birth weight. Importantly, women presenting with MiP commonly develop anaemia. In Ethiopia, the Ministry of Health recommends screening symptomatic women only at antenatal care visits with no formal intermittent preventive therapy. Since MiP can display low-level parasitaemia, current tests which include microscopy and RDT are challenged to detect these cases. Loop mediated isothermal Amplification (LAMP) technology is a highly sensitive technique for DNA detection and is field compatible. This study aims to evaluate the impact of active malaria case detection during pregnancy using LAMP technology in terms of birth outcomes. Methods A longitudinal study was conducted in two health centres of the Kafa zone, South West Ethiopia. Both symptomatic and asymptomatic pregnant women were enrolled in the first or second trimester and allocated to either Standard of Care (SOC—microscopy and RDT) or LAMP (LAMP, microscopy and RDT). Women completed at least three visits prior to delivery, and the patient was referred for treatment if Plasmodium infection was detected by any of the testing methods. The primary outcome was to measure absolute birth weight, proportion of low birth weight, and maternal/neonatal haemoglobin in each arm. Secondary outcomes were to assess the performance of microscopy and RDT versus LAMP conducted in the field. Results One hundred and ninety-nine women were included and assigned to either LAMP or SOC. Six were lost to follow up. In this cohort, 66.8% of women did not display any clinical symptoms and 70.9% were multi-parous. A reduced proportion of low birth weight newborns was observed in the LAMP group (0%) compared to standard of care (14%) (p <0.001). ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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Malaria Asymptomatic infections Pregnancy LAMP Low birth weight Anemia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Malaria Asymptomatic infections Pregnancy LAMP Low birth weight Anemia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Guluma Tadesse Claire Kamaliddin Cody Doolan Ranmalee Amarasekara Ruth Legese Abu Naser Mohon James Cheaveau Delenasaw Yewhalaw Dylan R. Pillai Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia |
topic_facet |
Malaria Asymptomatic infections Pregnancy LAMP Low birth weight Anemia Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background 125 million women are pregnant each year in malaria endemic areas and are, therefore, at risk of Malaria in Pregnancy (MiP). MiP is the direct consequence of Plasmodium infection during pregnancy. The sequestration of Plasmodium falciparum parasites in the placenta adversely affects fetal development and impacts newborn birth weight. Importantly, women presenting with MiP commonly develop anaemia. In Ethiopia, the Ministry of Health recommends screening symptomatic women only at antenatal care visits with no formal intermittent preventive therapy. Since MiP can display low-level parasitaemia, current tests which include microscopy and RDT are challenged to detect these cases. Loop mediated isothermal Amplification (LAMP) technology is a highly sensitive technique for DNA detection and is field compatible. This study aims to evaluate the impact of active malaria case detection during pregnancy using LAMP technology in terms of birth outcomes. Methods A longitudinal study was conducted in two health centres of the Kafa zone, South West Ethiopia. Both symptomatic and asymptomatic pregnant women were enrolled in the first or second trimester and allocated to either Standard of Care (SOC—microscopy and RDT) or LAMP (LAMP, microscopy and RDT). Women completed at least three visits prior to delivery, and the patient was referred for treatment if Plasmodium infection was detected by any of the testing methods. The primary outcome was to measure absolute birth weight, proportion of low birth weight, and maternal/neonatal haemoglobin in each arm. Secondary outcomes were to assess the performance of microscopy and RDT versus LAMP conducted in the field. Results One hundred and ninety-nine women were included and assigned to either LAMP or SOC. Six were lost to follow up. In this cohort, 66.8% of women did not display any clinical symptoms and 70.9% were multi-parous. A reduced proportion of low birth weight newborns was observed in the LAMP group (0%) compared to standard of care (14%) (p <0.001). ... |
format |
Article in Journal/Newspaper |
author |
Guluma Tadesse Claire Kamaliddin Cody Doolan Ranmalee Amarasekara Ruth Legese Abu Naser Mohon James Cheaveau Delenasaw Yewhalaw Dylan R. Pillai |
author_facet |
Guluma Tadesse Claire Kamaliddin Cody Doolan Ranmalee Amarasekara Ruth Legese Abu Naser Mohon James Cheaveau Delenasaw Yewhalaw Dylan R. Pillai |
author_sort |
Guluma Tadesse |
title |
Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia |
title_short |
Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia |
title_full |
Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia |
title_fullStr |
Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia |
title_full_unstemmed |
Active case detection of malaria in pregnancy using loop-mediated amplification (LAMP): a pilot outcomes study in South West Ethiopia |
title_sort |
active case detection of malaria in pregnancy using loop-mediated amplification (lamp): a pilot outcomes study in south west ethiopia |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03380-9 https://doaj.org/article/8b4c02d9f75e47dfac01f0210e8e0309 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-9 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03380-9 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03380-9 1475-2875 https://doaj.org/article/8b4c02d9f75e47dfac01f0210e8e0309 |
op_doi |
https://doi.org/10.1186/s12936-020-03380-9 |
container_title |
Malaria Journal |
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19 |
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1 |
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1766348141241565184 |