In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species.
Leishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenic Leishmania spp. as well as hundreds of strains, b...
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ftdoajarticles:oai:doaj.org/article:8b413dc9812e49aa8d38ea28c77505e7 2023-05-15T15:13:45+02:00 In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species. Nathalia Pinho Jacek R Wiśniewski Geovane Dias-Lopes Leonardo Saboia-Vahia Ana Cristina Souza Bombaça Camila Mesquita-Rodrigues Rubem Menna-Barreto Elisa Cupolillo Jose Batista de Jesus Gabriel Padrón Patricia Cuervo 2020-08-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008509 https://doaj.org/article/8b413dc9812e49aa8d38ea28c77505e7 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008509 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008509 https://doaj.org/article/8b413dc9812e49aa8d38ea28c77505e7 PLoS Neglected Tropical Diseases, Vol 14, Iss 8, p e0008509 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008509 2022-12-31T13:53:10Z Leishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenic Leishmania spp. as well as hundreds of strains, but large-scale proteomics analyses did not follow these achievements and the Leishmania proteome remained mostly uncharacterized. Here, we report a comprehensive comparative study of the proteomes of strains representing L. braziliensis, L. panamensis and L. guyanensis species. Proteins extracted by SDS-mediated lysis were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mass spectrometry. "Total Protein Approach" and "Proteomic Ruler" were applied for absolute quantification of proteins. Principal component analysis demonstrated very high reproducibility among biological replicates and a very clear differentiation of the three species. Our dataset comprises near 7000 proteins, representing the most complete Leishmania proteome yet known, and provides a comprehensive quantitative picture of the proteomes of the three species in terms of protein concentration and copy numbers. Analysis of the abundance of proteins from the major energy metabolic processes allow us to highlight remarkably differences among the species and suggest that these parasites depend on distinct energy substrates to obtain ATP. Whereas L. braziliensis relies the more on glycolysis, L. panamensis and L. guyanensis seem to depend mainly on mitochondrial respiration. These results were confirmed by biochemical assays showing opposite profiles for glucose uptake and O2 consumption in these species. In addition, we provide quantitative data about different membrane proteins, transporters, and lipids, all of which contribute for significant species-specific differences and provide rich substrate for explore new molecules for diagnosing purposes. Data are available via ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 8 e0008509 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Nathalia Pinho Jacek R Wiśniewski Geovane Dias-Lopes Leonardo Saboia-Vahia Ana Cristina Souza Bombaça Camila Mesquita-Rodrigues Rubem Menna-Barreto Elisa Cupolillo Jose Batista de Jesus Gabriel Padrón Patricia Cuervo In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Leishmania species are responsible for a broad spectrum of diseases, denominated Leishmaniasis, affecting over 12 million people worldwide. During the last decade, there have been impressive efforts for sequencing the genome of most of the pathogenic Leishmania spp. as well as hundreds of strains, but large-scale proteomics analyses did not follow these achievements and the Leishmania proteome remained mostly uncharacterized. Here, we report a comprehensive comparative study of the proteomes of strains representing L. braziliensis, L. panamensis and L. guyanensis species. Proteins extracted by SDS-mediated lysis were processed following the multi-enzyme digestion-filter aided sample preparation (FASP) procedure and analysed by high accuracy mass spectrometry. "Total Protein Approach" and "Proteomic Ruler" were applied for absolute quantification of proteins. Principal component analysis demonstrated very high reproducibility among biological replicates and a very clear differentiation of the three species. Our dataset comprises near 7000 proteins, representing the most complete Leishmania proteome yet known, and provides a comprehensive quantitative picture of the proteomes of the three species in terms of protein concentration and copy numbers. Analysis of the abundance of proteins from the major energy metabolic processes allow us to highlight remarkably differences among the species and suggest that these parasites depend on distinct energy substrates to obtain ATP. Whereas L. braziliensis relies the more on glycolysis, L. panamensis and L. guyanensis seem to depend mainly on mitochondrial respiration. These results were confirmed by biochemical assays showing opposite profiles for glucose uptake and O2 consumption in these species. In addition, we provide quantitative data about different membrane proteins, transporters, and lipids, all of which contribute for significant species-specific differences and provide rich substrate for explore new molecules for diagnosing purposes. Data are available via ... |
format |
Article in Journal/Newspaper |
author |
Nathalia Pinho Jacek R Wiśniewski Geovane Dias-Lopes Leonardo Saboia-Vahia Ana Cristina Souza Bombaça Camila Mesquita-Rodrigues Rubem Menna-Barreto Elisa Cupolillo Jose Batista de Jesus Gabriel Padrón Patricia Cuervo |
author_facet |
Nathalia Pinho Jacek R Wiśniewski Geovane Dias-Lopes Leonardo Saboia-Vahia Ana Cristina Souza Bombaça Camila Mesquita-Rodrigues Rubem Menna-Barreto Elisa Cupolillo Jose Batista de Jesus Gabriel Padrón Patricia Cuervo |
author_sort |
Nathalia Pinho |
title |
In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species. |
title_short |
In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species. |
title_full |
In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species. |
title_fullStr |
In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species. |
title_full_unstemmed |
In-depth quantitative proteomics uncovers specie-specific metabolic programs in Leishmania (Viannia) species. |
title_sort |
in-depth quantitative proteomics uncovers specie-specific metabolic programs in leishmania (viannia) species. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008509 https://doaj.org/article/8b413dc9812e49aa8d38ea28c77505e7 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 8, p e0008509 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008509 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008509 https://doaj.org/article/8b413dc9812e49aa8d38ea28c77505e7 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008509 |
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PLOS Neglected Tropical Diseases |
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14 |
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8 |
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e0008509 |
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