Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand

Abstract Background Dihydroartemisinin-piperaquine (DHA-PPQ) combination therapy is the current first-line treatment for Plasmodium falciparum malaria in Thailand. Since its introduction in 2015, resistance to this drug combination has emerged in the eastern part of the Greater Mekong Subregion incl...

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Published in:Malaria Journal
Main Authors: Khine Nwe Win, Khajohnpong Manopwisedjaroen, Kanit Phumchuea, Chayanut Suansomjit, Kesinee Chotivanich, Saranath Lawpoolsri, Liwang Cui, Jetsumon Sattabongkot, Wang Nguitragool
Format: Article in Journal/Newspaper
Language:English
Published: BMC 2022
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Online Access:https://doi.org/10.1186/s12936-022-04382-5
https://doaj.org/article/8b28966e15ad4723b7273dc09d6a5445
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spelling ftdoajarticles:oai:doaj.org/article:8b28966e15ad4723b7273dc09d6a5445 2023-05-15T15:11:09+02:00 Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand Khine Nwe Win Khajohnpong Manopwisedjaroen Kanit Phumchuea Chayanut Suansomjit Kesinee Chotivanich Saranath Lawpoolsri Liwang Cui Jetsumon Sattabongkot Wang Nguitragool 2022-11-01T00:00:00Z https://doi.org/10.1186/s12936-022-04382-5 https://doaj.org/article/8b28966e15ad4723b7273dc09d6a5445 EN eng BMC https://doi.org/10.1186/s12936-022-04382-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-022-04382-5 1475-2875 https://doaj.org/article/8b28966e15ad4723b7273dc09d6a5445 Malaria Journal, Vol 21, Iss 1, Pp 1-8 (2022) DHA-PPQ Plasmodium falciparum kelch13 Pfcrt plasmepsin-2 Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2022 ftdoajarticles https://doi.org/10.1186/s12936-022-04382-5 2022-12-30T20:09:14Z Abstract Background Dihydroartemisinin-piperaquine (DHA-PPQ) combination therapy is the current first-line treatment for Plasmodium falciparum malaria in Thailand. Since its introduction in 2015, resistance to this drug combination has emerged in the eastern part of the Greater Mekong Subregion including the eastern part of Thailand near Cambodia. This study aimed to assess whether the resistance genotypes have arisen the western part of country. Methods Fifty-seven P. falciparum-infected blood samples were collected in Tak province of northwestern Thailand between 2013 and 2019. Resistance to DHA was examined through the single nucleotide polymorphisms (SNPs) of kelch13. PPQ resistance was examined through the copy number plasmepsin-2 and the SNPs of Pfcrt. Results Among the samples whose kelch13 were successfully sequenced, approximately half (31/55; 56%) had mutation associated with artemisinin resistance, including G533S (23/55; 42%), C580Y (6/55; 11%), and G538V (2/55; 4%). During the study period, G533S mutation appeared and increased from 20% (4/20) in 2014 to 100% (9/9) in 2019. No plasmepsin-2 gene amplification was observed, but one sample (1/54) had the Pfcrt F145I mutation previously implicated in PPQ resistance. Conclusions Kelch13 mutation was common in Tak Province in 2013–2019. A new mutation G533S emerged in 2014 and rose to dominance in 2019. PPQ resistance marker Pfcrt F145I was also detected in 2019. Continued surveillance of treatment efficacy and drug resistance markers is warranted. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 21 1
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic DHA-PPQ
Plasmodium falciparum
kelch13
Pfcrt
plasmepsin-2
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle DHA-PPQ
Plasmodium falciparum
kelch13
Pfcrt
plasmepsin-2
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Khine Nwe Win
Khajohnpong Manopwisedjaroen
Kanit Phumchuea
Chayanut Suansomjit
Kesinee Chotivanich
Saranath Lawpoolsri
Liwang Cui
Jetsumon Sattabongkot
Wang Nguitragool
Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand
topic_facet DHA-PPQ
Plasmodium falciparum
kelch13
Pfcrt
plasmepsin-2
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Abstract Background Dihydroartemisinin-piperaquine (DHA-PPQ) combination therapy is the current first-line treatment for Plasmodium falciparum malaria in Thailand. Since its introduction in 2015, resistance to this drug combination has emerged in the eastern part of the Greater Mekong Subregion including the eastern part of Thailand near Cambodia. This study aimed to assess whether the resistance genotypes have arisen the western part of country. Methods Fifty-seven P. falciparum-infected blood samples were collected in Tak province of northwestern Thailand between 2013 and 2019. Resistance to DHA was examined through the single nucleotide polymorphisms (SNPs) of kelch13. PPQ resistance was examined through the copy number plasmepsin-2 and the SNPs of Pfcrt. Results Among the samples whose kelch13 were successfully sequenced, approximately half (31/55; 56%) had mutation associated with artemisinin resistance, including G533S (23/55; 42%), C580Y (6/55; 11%), and G538V (2/55; 4%). During the study period, G533S mutation appeared and increased from 20% (4/20) in 2014 to 100% (9/9) in 2019. No plasmepsin-2 gene amplification was observed, but one sample (1/54) had the Pfcrt F145I mutation previously implicated in PPQ resistance. Conclusions Kelch13 mutation was common in Tak Province in 2013–2019. A new mutation G533S emerged in 2014 and rose to dominance in 2019. PPQ resistance marker Pfcrt F145I was also detected in 2019. Continued surveillance of treatment efficacy and drug resistance markers is warranted.
format Article in Journal/Newspaper
author Khine Nwe Win
Khajohnpong Manopwisedjaroen
Kanit Phumchuea
Chayanut Suansomjit
Kesinee Chotivanich
Saranath Lawpoolsri
Liwang Cui
Jetsumon Sattabongkot
Wang Nguitragool
author_facet Khine Nwe Win
Khajohnpong Manopwisedjaroen
Kanit Phumchuea
Chayanut Suansomjit
Kesinee Chotivanich
Saranath Lawpoolsri
Liwang Cui
Jetsumon Sattabongkot
Wang Nguitragool
author_sort Khine Nwe Win
title Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand
title_short Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand
title_full Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand
title_fullStr Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand
title_full_unstemmed Molecular markers of dihydroartemisinin-piperaquine resistance in northwestern Thailand
title_sort molecular markers of dihydroartemisinin-piperaquine resistance in northwestern thailand
publisher BMC
publishDate 2022
url https://doi.org/10.1186/s12936-022-04382-5
https://doaj.org/article/8b28966e15ad4723b7273dc09d6a5445
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Malaria Journal, Vol 21, Iss 1, Pp 1-8 (2022)
op_relation https://doi.org/10.1186/s12936-022-04382-5
https://doaj.org/toc/1475-2875
doi:10.1186/s12936-022-04382-5
1475-2875
https://doaj.org/article/8b28966e15ad4723b7273dc09d6a5445
op_doi https://doi.org/10.1186/s12936-022-04382-5
container_title Malaria Journal
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