From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes.

BACKGROUND:The symbiotic bacterium Wolbachia is currently being trialled as a biocontrol agent in several countries to reduce dengue transmission. Wolbachia can invade and spread to infect all individuals within wild mosquito populations, but requires a high rate of maternal transmission, strong cyt...

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Published in:PLOS Neglected Tropical Diseases
Main Authors: Heverton Leandro Carneiro Dutra, Lilha Maria Barbosa Dos Santos, Eric Pearce Caragata, Jéssica Barreto Lopes Silva, Daniel Antunes Maciel Villela, Rafael Maciel-de-Freitas, Luciano Andrade Moreira
Format: Article in Journal/Newspaper
Language:English
Published: Public Library of Science (PLoS) 2015
Subjects:
Online Access:https://doi.org/10.1371/journal.pntd.0003689
https://doaj.org/article/8aebca606cd84924be600f43af14b993
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spelling ftdoajarticles:oai:doaj.org/article:8aebca606cd84924be600f43af14b993 2023-05-15T15:14:19+02:00 From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes. Heverton Leandro Carneiro Dutra Lilha Maria Barbosa Dos Santos Eric Pearce Caragata Jéssica Barreto Lopes Silva Daniel Antunes Maciel Villela Rafael Maciel-de-Freitas Luciano Andrade Moreira 2015-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003689 https://doaj.org/article/8aebca606cd84924be600f43af14b993 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4408005?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003689 https://doaj.org/article/8aebca606cd84924be600f43af14b993 PLoS Neglected Tropical Diseases, Vol 9, Iss 4, p e0003689 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003689 2022-12-31T05:33:52Z BACKGROUND:The symbiotic bacterium Wolbachia is currently being trialled as a biocontrol agent in several countries to reduce dengue transmission. Wolbachia can invade and spread to infect all individuals within wild mosquito populations, but requires a high rate of maternal transmission, strong cytoplasmic incompatibility and low fitness costs in the host in order to do so. Additionally, extensive differences in climate, field-release protocols, urbanization level and human density amongst the sites where this bacterium has been deployed have limited comparison and analysis of Wolbachia's invasive potential. METHODOLOGY/PRINCIPAL FINDINGS:We examined key phenotypic effects of the wMel Wolbachia strain in laboratory Aedes aegypti mosquitoes with a Brazilian genetic background to characterize its invasive potential. We show that the wMel strain causes strong cytoplasmic incompatibility, a high rate of maternal transmission and has no evident detrimental effect on host fecundity or fertility. Next, to understand the effects of different urban landscapes on the likelihood of mosquito survival, we performed mark-release-recapture experiments using Wolbachia-uninfected Brazilian mosquitoes in two areas of Rio de Janeiro where Wolbachia will be deployed in the future. We characterized the mosquito populations in relation to the socio-demographic conditions at these sites, and at three other future release areas. We then constructed mathematical models using both the laboratory and field data, and used these to describe the influence of urban environmental conditions on the likelihood that the Wolbachia infection frequency could reach 100% following mosquito release. We predict successful invasion at all five field sites, however the conditions by which this occurs vary greatly between sites, and are strongly influenced by the size of the local mosquito population. CONCLUSIONS/SIGNIFICANCE:Through analysis of laboratory, field and mathematical data, we show that the wMel strain of Wolbachia possesses the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 4 e0003689
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
spellingShingle Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
Heverton Leandro Carneiro Dutra
Lilha Maria Barbosa Dos Santos
Eric Pearce Caragata
Jéssica Barreto Lopes Silva
Daniel Antunes Maciel Villela
Rafael Maciel-de-Freitas
Luciano Andrade Moreira
From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes.
topic_facet Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
description BACKGROUND:The symbiotic bacterium Wolbachia is currently being trialled as a biocontrol agent in several countries to reduce dengue transmission. Wolbachia can invade and spread to infect all individuals within wild mosquito populations, but requires a high rate of maternal transmission, strong cytoplasmic incompatibility and low fitness costs in the host in order to do so. Additionally, extensive differences in climate, field-release protocols, urbanization level and human density amongst the sites where this bacterium has been deployed have limited comparison and analysis of Wolbachia's invasive potential. METHODOLOGY/PRINCIPAL FINDINGS:We examined key phenotypic effects of the wMel Wolbachia strain in laboratory Aedes aegypti mosquitoes with a Brazilian genetic background to characterize its invasive potential. We show that the wMel strain causes strong cytoplasmic incompatibility, a high rate of maternal transmission and has no evident detrimental effect on host fecundity or fertility. Next, to understand the effects of different urban landscapes on the likelihood of mosquito survival, we performed mark-release-recapture experiments using Wolbachia-uninfected Brazilian mosquitoes in two areas of Rio de Janeiro where Wolbachia will be deployed in the future. We characterized the mosquito populations in relation to the socio-demographic conditions at these sites, and at three other future release areas. We then constructed mathematical models using both the laboratory and field data, and used these to describe the influence of urban environmental conditions on the likelihood that the Wolbachia infection frequency could reach 100% following mosquito release. We predict successful invasion at all five field sites, however the conditions by which this occurs vary greatly between sites, and are strongly influenced by the size of the local mosquito population. CONCLUSIONS/SIGNIFICANCE:Through analysis of laboratory, field and mathematical data, we show that the wMel strain of Wolbachia possesses the ...
format Article in Journal/Newspaper
author Heverton Leandro Carneiro Dutra
Lilha Maria Barbosa Dos Santos
Eric Pearce Caragata
Jéssica Barreto Lopes Silva
Daniel Antunes Maciel Villela
Rafael Maciel-de-Freitas
Luciano Andrade Moreira
author_facet Heverton Leandro Carneiro Dutra
Lilha Maria Barbosa Dos Santos
Eric Pearce Caragata
Jéssica Barreto Lopes Silva
Daniel Antunes Maciel Villela
Rafael Maciel-de-Freitas
Luciano Andrade Moreira
author_sort Heverton Leandro Carneiro Dutra
title From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes.
title_short From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes.
title_full From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes.
title_fullStr From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes.
title_full_unstemmed From lab to field: the influence of urban landscapes on the invasive potential of Wolbachia in Brazilian Aedes aegypti mosquitoes.
title_sort from lab to field: the influence of urban landscapes on the invasive potential of wolbachia in brazilian aedes aegypti mosquitoes.
publisher Public Library of Science (PLoS)
publishDate 2015
url https://doi.org/10.1371/journal.pntd.0003689
https://doaj.org/article/8aebca606cd84924be600f43af14b993
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source PLoS Neglected Tropical Diseases, Vol 9, Iss 4, p e0003689 (2015)
op_relation http://europepmc.org/articles/PMC4408005?pdf=render
https://doaj.org/toc/1935-2727
https://doaj.org/toc/1935-2735
1935-2727
1935-2735
doi:10.1371/journal.pntd.0003689
https://doaj.org/article/8aebca606cd84924be600f43af14b993
op_doi https://doi.org/10.1371/journal.pntd.0003689
container_title PLOS Neglected Tropical Diseases
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