Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals
Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence i...
Published in: | Frontiers in Cell and Developmental Biology |
---|---|
Main Authors: | , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Frontiers Media S.A.
2023
|
Subjects: | |
Online Access: | https://doi.org/10.3389/fcell.2023.1050323 https://doaj.org/article/8ad8fd440912461192beda9d56c35283 |
id |
ftdoajarticles:oai:doaj.org/article:8ad8fd440912461192beda9d56c35283 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:8ad8fd440912461192beda9d56c35283 2023-05-15T15:26:24+02:00 Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida 2023-01-01T00:00:00Z https://doi.org/10.3389/fcell.2023.1050323 https://doaj.org/article/8ad8fd440912461192beda9d56c35283 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fcell.2023.1050323/full https://doaj.org/toc/2296-634X 2296-634X doi:10.3389/fcell.2023.1050323 https://doaj.org/article/8ad8fd440912461192beda9d56c35283 Frontiers in Cell and Developmental Biology, Vol 11 (2023) Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation Biology (General) QH301-705.5 article 2023 ftdoajarticles https://doi.org/10.3389/fcell.2023.1050323 2023-01-29T01:28:43Z Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence in jawed fish. However, sequencing of the Atlantic cod (Gadus morhua) genome revealed a loss of MHC class II genes, and an extreme expansion of MHC class I genes. These findings lead to the hypothesis that a loss of the MHC class II pathway coincided with a more versatile use of MHC class I, but so far there is no direct experimental evidence in support of this. To gain a deeper understanding of the function of the expanded MHC class I, we selected five MHC class I gene variants representing five of the six clades identified in previous studies and investigated their intracellular localization in human and Atlantic cod larval cells. Intriguingly, we uncovered that all selected MHC class I variants localize to endolysosomal compartments in Atlantic cod cells. Additionally, by introducing point mutations or deletions in the cytosolic tail, we found that hypothetical sorting signals in the MHC class I cytosolic tail do not influence MHC class I trafficking. Moreover, we demonstrated that in Atlantic cod, tapasin and MHC class I colocalize on endolysosomes suggesting that peptide-loading assistance and stabilization of MHC class I occurs outside the endoplasmic reticulum. Altogether, our results demonstrate that MHC class I from Atlantic cod is sorted to the endolysosomal system, which may indicate that it interacts with exogenous peptides for potential cross presentation. Article in Journal/Newspaper atlantic cod Gadus morhua Directory of Open Access Journals: DOAJ Articles Frontiers in Cell and Developmental Biology 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation Biology (General) QH301-705.5 |
spellingShingle |
Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation Biology (General) QH301-705.5 Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
topic_facet |
Atlantic cod (Gadus morhua) MHC I endolysosomes sorting signals cross-presentation Biology (General) QH301-705.5 |
description |
Major histocompatibility complex (MHC) class I and II are crucial for the adaptive immune system because they are involved in peptide presentation to T cells. Until recently, it was believed that MHC genes and their associated immune components had been conserved since their evolutionary emergence in jawed fish. However, sequencing of the Atlantic cod (Gadus morhua) genome revealed a loss of MHC class II genes, and an extreme expansion of MHC class I genes. These findings lead to the hypothesis that a loss of the MHC class II pathway coincided with a more versatile use of MHC class I, but so far there is no direct experimental evidence in support of this. To gain a deeper understanding of the function of the expanded MHC class I, we selected five MHC class I gene variants representing five of the six clades identified in previous studies and investigated their intracellular localization in human and Atlantic cod larval cells. Intriguingly, we uncovered that all selected MHC class I variants localize to endolysosomal compartments in Atlantic cod cells. Additionally, by introducing point mutations or deletions in the cytosolic tail, we found that hypothetical sorting signals in the MHC class I cytosolic tail do not influence MHC class I trafficking. Moreover, we demonstrated that in Atlantic cod, tapasin and MHC class I colocalize on endolysosomes suggesting that peptide-loading assistance and stabilization of MHC class I occurs outside the endoplasmic reticulum. Altogether, our results demonstrate that MHC class I from Atlantic cod is sorted to the endolysosomal system, which may indicate that it interacts with exogenous peptides for potential cross presentation. |
format |
Article in Journal/Newspaper |
author |
Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida |
author_facet |
Synne Arstad Bjørnestad Monica Hongrø Solbakken Kjetill S. Jakobsen Sissel Jentoft Oddmund Bakke Cinzia Progida |
author_sort |
Synne Arstad Bjørnestad |
title |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_short |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_full |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_fullStr |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_full_unstemmed |
Atlantic cod (Gadus morhua) MHC I localizes to endolysosomal compartments independently of cytosolic sorting signals |
title_sort |
atlantic cod (gadus morhua) mhc i localizes to endolysosomal compartments independently of cytosolic sorting signals |
publisher |
Frontiers Media S.A. |
publishDate |
2023 |
url |
https://doi.org/10.3389/fcell.2023.1050323 https://doaj.org/article/8ad8fd440912461192beda9d56c35283 |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Frontiers in Cell and Developmental Biology, Vol 11 (2023) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fcell.2023.1050323/full https://doaj.org/toc/2296-634X 2296-634X doi:10.3389/fcell.2023.1050323 https://doaj.org/article/8ad8fd440912461192beda9d56c35283 |
op_doi |
https://doi.org/10.3389/fcell.2023.1050323 |
container_title |
Frontiers in Cell and Developmental Biology |
container_volume |
11 |
_version_ |
1766356885947023360 |