Cellular immunity in women aged 20–40 living in different climatic and geographic regions
The aim. To identify phenotypic features of adaptive cellular immune responses in young women living in different climatic and geographic regions.Materials and methods. We examined 63 apparently healthy women, including 25 residents of Sovpolye settlement, Arkhangelsk region (Arctic region) and 38 r...
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Scientific Сentre for Family Health and Human Reproduction Problems
2024
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Online Access: | https://doi.org/10.29413/ABS.2024-9.2.12 https://doaj.org/article/8ace16daf49e4d6a918680d1a1a90bd4 |
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ftdoajarticles:oai:doaj.org/article:8ace16daf49e4d6a918680d1a1a90bd4 2024-09-15T17:54:46+00:00 Cellular immunity in women aged 20–40 living in different climatic and geographic regions L. S. Shchegoleva E. Yu. Shashkova T. B. Sergeeva 2024-06-01T00:00:00Z https://doi.org/10.29413/ABS.2024-9.2.12 https://doaj.org/article/8ace16daf49e4d6a918680d1a1a90bd4 RU rus Scientific Сentre for Family Health and Human Reproduction Problems https://www.actabiomedica.ru/jour/article/view/4734 https://doaj.org/toc/2541-9420 https://doaj.org/toc/2587-9596 2541-9420 2587-9596 doi:10.29413/ABS.2024-9.2.12 https://doaj.org/article/8ace16daf49e4d6a918680d1a1a90bd4 Acta Biomedica Scientifica, Vol 9, Iss 2, Pp 120-129 (2024) arctic region southern region adaptive immunity cytokines reserve capabilities of immune homeostasis Science Q article 2024 ftdoajarticles https://doi.org/10.29413/ABS.2024-9.2.12 2024-08-05T17:49:16Z The aim. To identify phenotypic features of adaptive cellular immune responses in young women living in different climatic and geographic regions.Materials and methods. We examined 63 apparently healthy women, including 25 residents of Sovpolye settlement, Arkhangelsk region (Arctic region) and 38 residents of Tskhinvali (Republic of South Ossetia) aged 20–40. The content of lymphocytes, their phenotypes CD4+, CD8+, CD3+, CD5+, CD16+, CD10+, CD71+, CD25+, HLA-DR+ and CD95+, CEA (carcinoembryonic antigen) glycoprotein, and interleukin (IL) 6 and IL-10 cytokines was studied. Lymphocytes phenotyping was performed by indirect immunoperoxidase reaction using monoclonal antibodies (MedBioSpektr, Moscow) on “dried drop” lymphocyte sample with peroxidase conjugate and chromogen solution staining for immersion microscopy analysis (Nicon 50i, Japan). The content of CEA glycoprotein and cytokines was determined using ELISA.Results. In women living in Arctic region, a deficiency of CD5+, CD3+, CD10+, CD95+, CD71+, CD25+, HLA-DR+ cells, CEA and IL-6 concentrations is associated with high concentrations of CD8+ and IL-10. In women living in Southern region, a slight deficiency of CD5+, CD8+ and CD95+ cells is associated with high concentrations of CEA, IL-6, IL-10 and CD10+, CD16+ and HLA-DR+ cells.Conclusion. In inhabitants of different climatic regions, the features of formation of adaptive immune reactions are determined by different quantitative and qualitative composition of lymphocytes and cytokines phenotypes. Reduction of reserve capabilities of immune homeostasis is detected 3 times more often in inhabitants of the Arctic region. Article in Journal/Newspaper Arkhangelsk Directory of Open Access Journals: DOAJ Articles Acta Biomedica Scientifica 9 2 120 129 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
Russian |
topic |
arctic region southern region adaptive immunity cytokines reserve capabilities of immune homeostasis Science Q |
spellingShingle |
arctic region southern region adaptive immunity cytokines reserve capabilities of immune homeostasis Science Q L. S. Shchegoleva E. Yu. Shashkova T. B. Sergeeva Cellular immunity in women aged 20–40 living in different climatic and geographic regions |
topic_facet |
arctic region southern region adaptive immunity cytokines reserve capabilities of immune homeostasis Science Q |
description |
The aim. To identify phenotypic features of adaptive cellular immune responses in young women living in different climatic and geographic regions.Materials and methods. We examined 63 apparently healthy women, including 25 residents of Sovpolye settlement, Arkhangelsk region (Arctic region) and 38 residents of Tskhinvali (Republic of South Ossetia) aged 20–40. The content of lymphocytes, their phenotypes CD4+, CD8+, CD3+, CD5+, CD16+, CD10+, CD71+, CD25+, HLA-DR+ and CD95+, CEA (carcinoembryonic antigen) glycoprotein, and interleukin (IL) 6 and IL-10 cytokines was studied. Lymphocytes phenotyping was performed by indirect immunoperoxidase reaction using monoclonal antibodies (MedBioSpektr, Moscow) on “dried drop” lymphocyte sample with peroxidase conjugate and chromogen solution staining for immersion microscopy analysis (Nicon 50i, Japan). The content of CEA glycoprotein and cytokines was determined using ELISA.Results. In women living in Arctic region, a deficiency of CD5+, CD3+, CD10+, CD95+, CD71+, CD25+, HLA-DR+ cells, CEA and IL-6 concentrations is associated with high concentrations of CD8+ and IL-10. In women living in Southern region, a slight deficiency of CD5+, CD8+ and CD95+ cells is associated with high concentrations of CEA, IL-6, IL-10 and CD10+, CD16+ and HLA-DR+ cells.Conclusion. In inhabitants of different climatic regions, the features of formation of adaptive immune reactions are determined by different quantitative and qualitative composition of lymphocytes and cytokines phenotypes. Reduction of reserve capabilities of immune homeostasis is detected 3 times more often in inhabitants of the Arctic region. |
format |
Article in Journal/Newspaper |
author |
L. S. Shchegoleva E. Yu. Shashkova T. B. Sergeeva |
author_facet |
L. S. Shchegoleva E. Yu. Shashkova T. B. Sergeeva |
author_sort |
L. S. Shchegoleva |
title |
Cellular immunity in women aged 20–40 living in different climatic and geographic regions |
title_short |
Cellular immunity in women aged 20–40 living in different climatic and geographic regions |
title_full |
Cellular immunity in women aged 20–40 living in different climatic and geographic regions |
title_fullStr |
Cellular immunity in women aged 20–40 living in different climatic and geographic regions |
title_full_unstemmed |
Cellular immunity in women aged 20–40 living in different climatic and geographic regions |
title_sort |
cellular immunity in women aged 20–40 living in different climatic and geographic regions |
publisher |
Scientific Сentre for Family Health and Human Reproduction Problems |
publishDate |
2024 |
url |
https://doi.org/10.29413/ABS.2024-9.2.12 https://doaj.org/article/8ace16daf49e4d6a918680d1a1a90bd4 |
genre |
Arkhangelsk |
genre_facet |
Arkhangelsk |
op_source |
Acta Biomedica Scientifica, Vol 9, Iss 2, Pp 120-129 (2024) |
op_relation |
https://www.actabiomedica.ru/jour/article/view/4734 https://doaj.org/toc/2541-9420 https://doaj.org/toc/2587-9596 2541-9420 2587-9596 doi:10.29413/ABS.2024-9.2.12 https://doaj.org/article/8ace16daf49e4d6a918680d1a1a90bd4 |
op_doi |
https://doi.org/10.29413/ABS.2024-9.2.12 |
container_title |
Acta Biomedica Scientifica |
container_volume |
9 |
container_issue |
2 |
container_start_page |
120 |
op_container_end_page |
129 |
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1810431077417746432 |