Rapid identification of black grain eumycetoma causative agents using rolling circle amplification.
Accurate identification of mycetoma causative agent is a priority for treatment. However, current identification tools are far from being satisfactory for both reliable diagnosis and epidemiological investigations. A rapid, simple, and highly efficient molecular based method for identification of ag...
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ftdoajarticles:oai:doaj.org/article:8a7b200df6654b7c991c547e329e4fa1 2023-05-15T15:03:11+02:00 Rapid identification of black grain eumycetoma causative agents using rolling circle amplification. Sarah A Ahmed Bert H G Gerrits van den Ende Ahmed H Fahal Wendy W J van de Sande G S de Hoog 2014-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003368 https://doaj.org/article/8a7b200df6654b7c991c547e329e4fa1 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4256478?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003368 https://doaj.org/article/8a7b200df6654b7c991c547e329e4fa1 PLoS Neglected Tropical Diseases, Vol 8, Iss 12, p e3368 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003368 2022-12-31T14:37:06Z Accurate identification of mycetoma causative agent is a priority for treatment. However, current identification tools are far from being satisfactory for both reliable diagnosis and epidemiological investigations. A rapid, simple, and highly efficient molecular based method for identification of agents of black grain eumycetoma is introduced, aiming to improve diagnostic in endemic areas. Rolling Circle Amplification (RCA) uses species-specific padlock probes and isothermal DNA amplification. The tests were based on ITS sequences and developed for Falciformispora senegalensis, F. tompkinsii, Madurella fahalii, M. mycetomatis, M. pseudomycetomatis, M. tropicana, Medicopsis romeroi, and Trematosphaeria grisea. With the isothermal RCA assay, 62 isolates were successfully identified with 100% specificity and no cross reactivity or false results. The main advantage of this technique is the low-cost, high specificity, and simplicity. In addition, it is highly reproducible and can be performed within a single day. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 12 e3368 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Sarah A Ahmed Bert H G Gerrits van den Ende Ahmed H Fahal Wendy W J van de Sande G S de Hoog Rapid identification of black grain eumycetoma causative agents using rolling circle amplification. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Accurate identification of mycetoma causative agent is a priority for treatment. However, current identification tools are far from being satisfactory for both reliable diagnosis and epidemiological investigations. A rapid, simple, and highly efficient molecular based method for identification of agents of black grain eumycetoma is introduced, aiming to improve diagnostic in endemic areas. Rolling Circle Amplification (RCA) uses species-specific padlock probes and isothermal DNA amplification. The tests were based on ITS sequences and developed for Falciformispora senegalensis, F. tompkinsii, Madurella fahalii, M. mycetomatis, M. pseudomycetomatis, M. tropicana, Medicopsis romeroi, and Trematosphaeria grisea. With the isothermal RCA assay, 62 isolates were successfully identified with 100% specificity and no cross reactivity or false results. The main advantage of this technique is the low-cost, high specificity, and simplicity. In addition, it is highly reproducible and can be performed within a single day. |
format |
Article in Journal/Newspaper |
author |
Sarah A Ahmed Bert H G Gerrits van den Ende Ahmed H Fahal Wendy W J van de Sande G S de Hoog |
author_facet |
Sarah A Ahmed Bert H G Gerrits van den Ende Ahmed H Fahal Wendy W J van de Sande G S de Hoog |
author_sort |
Sarah A Ahmed |
title |
Rapid identification of black grain eumycetoma causative agents using rolling circle amplification. |
title_short |
Rapid identification of black grain eumycetoma causative agents using rolling circle amplification. |
title_full |
Rapid identification of black grain eumycetoma causative agents using rolling circle amplification. |
title_fullStr |
Rapid identification of black grain eumycetoma causative agents using rolling circle amplification. |
title_full_unstemmed |
Rapid identification of black grain eumycetoma causative agents using rolling circle amplification. |
title_sort |
rapid identification of black grain eumycetoma causative agents using rolling circle amplification. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003368 https://doaj.org/article/8a7b200df6654b7c991c547e329e4fa1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 12, p e3368 (2014) |
op_relation |
http://europepmc.org/articles/PMC4256478?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003368 https://doaj.org/article/8a7b200df6654b7c991c547e329e4fa1 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003368 |
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PLoS Neglected Tropical Diseases |
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8 |
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12 |
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e3368 |
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