Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions
Increased research attention is being given to bacterial diversity associated with lichens. Rock tripe lichens ( Umbilicariaceae ) were collected from two distinct Antarctic biological regions, the continental region near the Japanese Antarctic station (Syowa Station) and the maritime Antarctic Sout...
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ftdoajarticles:oai:doaj.org/article:8a69a5a8362744698377307976c00e82 2024-01-07T09:39:48+01:00 Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions Zichen He Takeshi Naganuma Ryosuke Nakai Satoshi Imura Megumu Tsujimoto Peter Convey 2022-08-01T00:00:00Z https://doi.org/10.3390/jof8080817 https://doaj.org/article/8a69a5a8362744698377307976c00e82 EN eng MDPI AG https://www.mdpi.com/2309-608X/8/8/817 https://doaj.org/toc/2309-608X doi:10.3390/jof8080817 2309-608X https://doaj.org/article/8a69a5a8362744698377307976c00e82 Journal of Fungi, Vol 8, Iss 8, p 817 (2022) Umbilicaria Antarctica rRNA gene V3-V4 region MiSeq OTUs Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/jof8080817 2023-12-10T01:44:29Z Increased research attention is being given to bacterial diversity associated with lichens. Rock tripe lichens ( Umbilicariaceae ) were collected from two distinct Antarctic biological regions, the continental region near the Japanese Antarctic station (Syowa Station) and the maritime Antarctic South Orkney Islands (Signy Island), in order to compare their bacterial floras and potential metabolism. Bulk DNA extracted from the lichen samples was used to amplify the 18S rRNA gene and the V3-V4 region of the 16S rRNA gene, whose amplicons were Sanger- and MiSeq-sequenced, respectively. The fungal and algal partners represented members of the ascomycete genus Umbilicaria and the green algal genus Trebouxia , based on 18S rRNA gene sequences. The V3-V4 sequences were grouped into operational taxonomic units (OTUs), which were assigned to eight bacterial phyla, Acidobacteriota , Actinomyceota , Armatimonadota , Bacteroidota , Cyanobacteria , Deinococcota , Pseudomonadota and the candidate phylum Saccharibacteria (also known as TM7), commonly present in all samples. The OTU floras of the two biological regions were clearly distinct, with regional biomarker genera, such as Mucilaginibacter and Gluconacetobacter , respectively. The OTU-based metabolism analysis predicted higher membrane transport activities in the maritime Antarctic OTUs, probably influenced by the sampling area’s warmer maritime climatic setting. Article in Journal/Newspaper Antarc* Antarctic Antarctica Signy Island South Orkney Islands Directory of Open Access Journals: DOAJ Articles Antarctic Signy Island ENVELOPE(-45.595,-45.595,-60.708,-60.708) South Orkney Islands ENVELOPE(-45.500,-45.500,-60.583,-60.583) Syowa Station Journal of Fungi 8 8 817 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Umbilicaria Antarctica rRNA gene V3-V4 region MiSeq OTUs Biology (General) QH301-705.5 |
spellingShingle |
Umbilicaria Antarctica rRNA gene V3-V4 region MiSeq OTUs Biology (General) QH301-705.5 Zichen He Takeshi Naganuma Ryosuke Nakai Satoshi Imura Megumu Tsujimoto Peter Convey Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
topic_facet |
Umbilicaria Antarctica rRNA gene V3-V4 region MiSeq OTUs Biology (General) QH301-705.5 |
description |
Increased research attention is being given to bacterial diversity associated with lichens. Rock tripe lichens ( Umbilicariaceae ) were collected from two distinct Antarctic biological regions, the continental region near the Japanese Antarctic station (Syowa Station) and the maritime Antarctic South Orkney Islands (Signy Island), in order to compare their bacterial floras and potential metabolism. Bulk DNA extracted from the lichen samples was used to amplify the 18S rRNA gene and the V3-V4 region of the 16S rRNA gene, whose amplicons were Sanger- and MiSeq-sequenced, respectively. The fungal and algal partners represented members of the ascomycete genus Umbilicaria and the green algal genus Trebouxia , based on 18S rRNA gene sequences. The V3-V4 sequences were grouped into operational taxonomic units (OTUs), which were assigned to eight bacterial phyla, Acidobacteriota , Actinomyceota , Armatimonadota , Bacteroidota , Cyanobacteria , Deinococcota , Pseudomonadota and the candidate phylum Saccharibacteria (also known as TM7), commonly present in all samples. The OTU floras of the two biological regions were clearly distinct, with regional biomarker genera, such as Mucilaginibacter and Gluconacetobacter , respectively. The OTU-based metabolism analysis predicted higher membrane transport activities in the maritime Antarctic OTUs, probably influenced by the sampling area’s warmer maritime climatic setting. |
format |
Article in Journal/Newspaper |
author |
Zichen He Takeshi Naganuma Ryosuke Nakai Satoshi Imura Megumu Tsujimoto Peter Convey |
author_facet |
Zichen He Takeshi Naganuma Ryosuke Nakai Satoshi Imura Megumu Tsujimoto Peter Convey |
author_sort |
Zichen He |
title |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_short |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_full |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_fullStr |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_full_unstemmed |
Microbiomic Analysis of Bacteria Associated with Rock Tripe Lichens in Continental and Maritime Antarctic Regions |
title_sort |
microbiomic analysis of bacteria associated with rock tripe lichens in continental and maritime antarctic regions |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/jof8080817 https://doaj.org/article/8a69a5a8362744698377307976c00e82 |
long_lat |
ENVELOPE(-45.595,-45.595,-60.708,-60.708) ENVELOPE(-45.500,-45.500,-60.583,-60.583) |
geographic |
Antarctic Signy Island South Orkney Islands Syowa Station |
geographic_facet |
Antarctic Signy Island South Orkney Islands Syowa Station |
genre |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
genre_facet |
Antarc* Antarctic Antarctica Signy Island South Orkney Islands |
op_source |
Journal of Fungi, Vol 8, Iss 8, p 817 (2022) |
op_relation |
https://www.mdpi.com/2309-608X/8/8/817 https://doaj.org/toc/2309-608X doi:10.3390/jof8080817 2309-608X https://doaj.org/article/8a69a5a8362744698377307976c00e82 |
op_doi |
https://doi.org/10.3390/jof8080817 |
container_title |
Journal of Fungi |
container_volume |
8 |
container_issue |
8 |
container_start_page |
817 |
_version_ |
1787430060246958080 |