Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon
Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antivi...
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ftdoajarticles:oai:doaj.org/article:8a3d016e4d92456d9c174573122562d2 2023-05-15T15:32:19+02:00 Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon Constanza Cárdenas Fanny Guzmán Marisela Carmona Cristian Muñoz Luis Nilo Alvaro Labra Sergio H. Marshall 2020-07-01T00:00:00Z https://doi.org/10.3390/pathogens9080600 https://doaj.org/article/8a3d016e4d92456d9c174573122562d2 EN eng MDPI AG https://www.mdpi.com/2076-0817/9/8/600 https://doaj.org/toc/2076-0817 doi:10.3390/pathogens9080600 2076-0817 https://doaj.org/article/8a3d016e4d92456d9c174573122562d2 Pathogens, Vol 9, Iss 600, p 600 (2020) interfering peptides viral treatment RNA fish viruses Medicine R article 2020 ftdoajarticles https://doi.org/10.3390/pathogens9080600 2022-12-30T22:29:14Z Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles Pathogens 9 8 600 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
interfering peptides viral treatment RNA fish viruses Medicine R |
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interfering peptides viral treatment RNA fish viruses Medicine R Constanza Cárdenas Fanny Guzmán Marisela Carmona Cristian Muñoz Luis Nilo Alvaro Labra Sergio H. Marshall Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
topic_facet |
interfering peptides viral treatment RNA fish viruses Medicine R |
description |
Viral infections in salmonids represent an ongoing challenge for the aquaculture industry. Two RNA viruses, the infectious pancreatic necrosis virus (IPNV) and the infectious salmon anemia virus (ISAV), have become a latent risk without healing therapies available for either. In this context, antiviral peptides emerge as effective and relatively safe therapeutic molecules. Based on in silico analysis of VP2 protein from IPNV and the RNA-dependent RNA polymerase from ISAV, a set of peptides was designed and were chemically synthesized to block selected key events in their corresponding infectivity processes. The peptides were tested in fish cell lines in vitro, and four were selected for decreasing the viral load: peptide GIM182 for IPNV, and peptides GIM535, GIM538 and GIM539 for ISAV. In vivo tests with the IPNV GIM 182 peptide were carried out using Salmo salar fish, showing a significant decrease of viral load, and proving the safety of the peptide for fish. The results indicate that the use of peptides as antiviral agents in disease control might be a viable alternative to explore in aquaculture. |
format |
Article in Journal/Newspaper |
author |
Constanza Cárdenas Fanny Guzmán Marisela Carmona Cristian Muñoz Luis Nilo Alvaro Labra Sergio H. Marshall |
author_facet |
Constanza Cárdenas Fanny Guzmán Marisela Carmona Cristian Muñoz Luis Nilo Alvaro Labra Sergio H. Marshall |
author_sort |
Constanza Cárdenas |
title |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_short |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_full |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_fullStr |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_full_unstemmed |
Synthetic Peptides as a Promising Alternative to Control Viral Infections in Atlantic Salmon |
title_sort |
synthetic peptides as a promising alternative to control viral infections in atlantic salmon |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/pathogens9080600 https://doaj.org/article/8a3d016e4d92456d9c174573122562d2 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
Pathogens, Vol 9, Iss 600, p 600 (2020) |
op_relation |
https://www.mdpi.com/2076-0817/9/8/600 https://doaj.org/toc/2076-0817 doi:10.3390/pathogens9080600 2076-0817 https://doaj.org/article/8a3d016e4d92456d9c174573122562d2 |
op_doi |
https://doi.org/10.3390/pathogens9080600 |
container_title |
Pathogens |
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9 |
container_issue |
8 |
container_start_page |
600 |
_version_ |
1766362836080001024 |