In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes.
Abstract: INTRODUCTION: Dengue fever is a viral disease transmitted by the Aedes aegypti Linn. (1792) (Diptera: Culicidae) mosquito, which is endemic in several regions of Brazil. Alternative methods for the control of the vector include botanical insecticides, which offer advantages such as lower e...
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ftdoajarticles:oai:doaj.org/article:89d63e785f8040fabc76c8854bdb0a04 2023-05-15T15:08:23+02:00 In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes. Karla Rejane de Andrade Porto Priscilla Rezende Motti Alexandre Alves Machado Antonia Railda Roel https://doi.org/10.1590/0037-8682-0117-2016 https://doaj.org/article/89d63e785f8040fabc76c8854bdb0a04 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000600693&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0117-2016 https://doaj.org/article/89d63e785f8040fabc76c8854bdb0a04 Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 6, Pp 693-697 Cytotoxicity Botanical insecticide Dengue fever Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0117-2016 2022-12-30T21:39:25Z Abstract: INTRODUCTION: Dengue fever is a viral disease transmitted by the Aedes aegypti Linn. (1792) (Diptera: Culicidae) mosquito, which is endemic in several regions of Brazil. Alternative methods for the control of the vector include botanical insecticides, which offer advantages such as lower environmental contamination levels and less likelihood of resistant populations. Thus, in this study, the ability of botanical insecticide formulations to inhibit the activity of the liver enzymes serum cholinesterase and malate dehydrogenase was evaluated. METHODS: Inhibition profiles were assessed using in vitro assays for cholinesterase and malate dehydrogenase activity and quantitated by ultraviolet-visible spectroscopy at 410nm to 340nm. RESULTS Insecticide products formulated from cashew nutshell liquid [A] and ricinoleic acid [B] showed cholinesterase activity levels of 6.26IU/mL and 6.61IU/mL, respectively, while the control level for cholinesterase was 5-12IU/mL. The products did not affect the level of 0.44IU/mL established for malate dehydrogenase, as the levels produced by [A] and [B] were 0.43IU/mL and 0.45IU/mL, respectively. CONCLUSIONS Our findings show that in vitro testing of the formulated products at concentrations lethal to A. aegypti did not affect the activity of cholinesterase and malate dehydrogenase, indicating the safety of these products. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 49 6 693 697 |
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topic |
Cytotoxicity Botanical insecticide Dengue fever Arctic medicine. Tropical medicine RC955-962 |
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Cytotoxicity Botanical insecticide Dengue fever Arctic medicine. Tropical medicine RC955-962 Karla Rejane de Andrade Porto Priscilla Rezende Motti Alexandre Alves Machado Antonia Railda Roel In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes. |
topic_facet |
Cytotoxicity Botanical insecticide Dengue fever Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract: INTRODUCTION: Dengue fever is a viral disease transmitted by the Aedes aegypti Linn. (1792) (Diptera: Culicidae) mosquito, which is endemic in several regions of Brazil. Alternative methods for the control of the vector include botanical insecticides, which offer advantages such as lower environmental contamination levels and less likelihood of resistant populations. Thus, in this study, the ability of botanical insecticide formulations to inhibit the activity of the liver enzymes serum cholinesterase and malate dehydrogenase was evaluated. METHODS: Inhibition profiles were assessed using in vitro assays for cholinesterase and malate dehydrogenase activity and quantitated by ultraviolet-visible spectroscopy at 410nm to 340nm. RESULTS Insecticide products formulated from cashew nutshell liquid [A] and ricinoleic acid [B] showed cholinesterase activity levels of 6.26IU/mL and 6.61IU/mL, respectively, while the control level for cholinesterase was 5-12IU/mL. The products did not affect the level of 0.44IU/mL established for malate dehydrogenase, as the levels produced by [A] and [B] were 0.43IU/mL and 0.45IU/mL, respectively. CONCLUSIONS Our findings show that in vitro testing of the formulated products at concentrations lethal to A. aegypti did not affect the activity of cholinesterase and malate dehydrogenase, indicating the safety of these products. |
format |
Article in Journal/Newspaper |
author |
Karla Rejane de Andrade Porto Priscilla Rezende Motti Alexandre Alves Machado Antonia Railda Roel |
author_facet |
Karla Rejane de Andrade Porto Priscilla Rezende Motti Alexandre Alves Machado Antonia Railda Roel |
author_sort |
Karla Rejane de Andrade Porto |
title |
In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes. |
title_short |
In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes. |
title_full |
In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes. |
title_fullStr |
In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes. |
title_full_unstemmed |
In vitro evaluation of the effect of botanical formulations used in the control of Aedes aegypti L. (Diptera: Culicidae) on liver enzymes. |
title_sort |
in vitro evaluation of the effect of botanical formulations used in the control of aedes aegypti l. (diptera: culicidae) on liver enzymes. |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0117-2016 https://doaj.org/article/89d63e785f8040fabc76c8854bdb0a04 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 49, Iss 6, Pp 693-697 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822016000600693&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0117-2016 https://doaj.org/article/89d63e785f8040fabc76c8854bdb0a04 |
op_doi |
https://doi.org/10.1590/0037-8682-0117-2016 |
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Revista da Sociedade Brasileira de Medicina Tropical |
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49 |
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