Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS
Objective: To identify the bioactive compounds in catechins isolation and its components from green tea GMB-4 clone. Methods: Green tea GMB-4 clones were extracted with distilled water at 90 °C. Samples were eluted into the column with 10% ethanol. Subsequently, the column was eluted with 95% ethano...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wolters Kluwer Medknow Publications
2015
|
Subjects: | |
Online Access: | https://doi.org/10.1016/j.apjtb.2015.09.013 https://doaj.org/article/89953d5cdadc4ec287e6bf4eba78f5d0 |
id |
ftdoajarticles:oai:doaj.org/article:89953d5cdadc4ec287e6bf4eba78f5d0 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:89953d5cdadc4ec287e6bf4eba78f5d0 2023-05-15T15:13:09+02:00 Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS Erna Susanti Ciptati Retty Ratnawati Aulanni'am Achmad Rudijanto 2015-12-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.09.013 https://doaj.org/article/89953d5cdadc4ec287e6bf4eba78f5d0 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115002257 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.09.013 https://doaj.org/article/89953d5cdadc4ec287e6bf4eba78f5d0 Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 1046-1050 (2015) Catechins Green tea GMB-4 clone LC-MS/MS HPLC Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2015 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.09.013 2022-12-31T00:28:21Z Objective: To identify the bioactive compounds in catechins isolation and its components from green tea GMB-4 clone. Methods: Green tea GMB-4 clones were extracted with distilled water at 90 °C. Samples were eluted into the column with 10% ethanol. Subsequently, the column was eluted with 95% ethanol and evaporated separately. Green tea extract was identified by thin layer chromatography. Catechins were separated by the stationary phase in column chromatography using polyamide with 10% ethanol eluent and 95% ethanol. The results of isolations were analyzed by high performance liquid chromatographic (HPLC) and LC-MS/MS. Analysis of catechins by HPLC was done by external standard. Results: Fraction from 10% ethanol showed that four major peaks at retention time of 1.663, 2.367, 2.950 and 4.890, indicated the presence of four catechins components including catechin, epicatechins, gallocatechin and epigallocatechin. Whereas, fraction from 95% ethanol showed two main peaks at retention time of 5.167 and 9.82, which indicated the presence of epigallocatechin gallate (EGCG) and epicatechin gallate (ECG). EGCG (m/z 459), epigallocatechin (m/z 307), ECG (m/z 443), and epicatechin (m/z 291) were isolated and separated successfully using HPLC and LC-MS/MS. Conclusions: The HPLC and LC-MS/MS methods were successfully tuned for the qualitative analysis of green tea extract with EGCG and ECG. Four major catechins were separated and identified by LC-MS/MS, such as EGCG, epigallocatechin, ECG and epicatechin. The result of HPLC analysis showed that EGCG and ECG were main components from catechins isolation of green tea GMB-4 clone. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 5 12 1046 1050 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Catechins Green tea GMB-4 clone LC-MS/MS HPLC Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Catechins Green tea GMB-4 clone LC-MS/MS HPLC Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Erna Susanti Ciptati Retty Ratnawati Aulanni'am Achmad Rudijanto Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS |
topic_facet |
Catechins Green tea GMB-4 clone LC-MS/MS HPLC Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To identify the bioactive compounds in catechins isolation and its components from green tea GMB-4 clone. Methods: Green tea GMB-4 clones were extracted with distilled water at 90 °C. Samples were eluted into the column with 10% ethanol. Subsequently, the column was eluted with 95% ethanol and evaporated separately. Green tea extract was identified by thin layer chromatography. Catechins were separated by the stationary phase in column chromatography using polyamide with 10% ethanol eluent and 95% ethanol. The results of isolations were analyzed by high performance liquid chromatographic (HPLC) and LC-MS/MS. Analysis of catechins by HPLC was done by external standard. Results: Fraction from 10% ethanol showed that four major peaks at retention time of 1.663, 2.367, 2.950 and 4.890, indicated the presence of four catechins components including catechin, epicatechins, gallocatechin and epigallocatechin. Whereas, fraction from 95% ethanol showed two main peaks at retention time of 5.167 and 9.82, which indicated the presence of epigallocatechin gallate (EGCG) and epicatechin gallate (ECG). EGCG (m/z 459), epigallocatechin (m/z 307), ECG (m/z 443), and epicatechin (m/z 291) were isolated and separated successfully using HPLC and LC-MS/MS. Conclusions: The HPLC and LC-MS/MS methods were successfully tuned for the qualitative analysis of green tea extract with EGCG and ECG. Four major catechins were separated and identified by LC-MS/MS, such as EGCG, epigallocatechin, ECG and epicatechin. The result of HPLC analysis showed that EGCG and ECG were main components from catechins isolation of green tea GMB-4 clone. |
format |
Article in Journal/Newspaper |
author |
Erna Susanti Ciptati Retty Ratnawati Aulanni'am Achmad Rudijanto |
author_facet |
Erna Susanti Ciptati Retty Ratnawati Aulanni'am Achmad Rudijanto |
author_sort |
Erna Susanti |
title |
Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS |
title_short |
Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS |
title_full |
Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS |
title_fullStr |
Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS |
title_full_unstemmed |
Qualitative analysis of catechins from green tea GMB-4 clone using HPLC and LC-MS/MS |
title_sort |
qualitative analysis of catechins from green tea gmb-4 clone using hplc and lc-ms/ms |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2015 |
url |
https://doi.org/10.1016/j.apjtb.2015.09.013 https://doaj.org/article/89953d5cdadc4ec287e6bf4eba78f5d0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 5, Iss 12, Pp 1046-1050 (2015) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115002257 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.09.013 https://doaj.org/article/89953d5cdadc4ec287e6bf4eba78f5d0 |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.09.013 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
5 |
container_issue |
12 |
container_start_page |
1046 |
op_container_end_page |
1050 |
_version_ |
1766343741816176640 |