Hematological indicators of rats for artificial hypobiosis

• Published in: ScienceRise: Biological Science
• Main Authors: Anna Umanska, Dmytro Melnichuk, Lilia Kalachnyuk
• Format: Article in Journal/Newspaper
• Language: English; Russian; Ukrainian
• Published: PC Technology Center 2017
• Subjects: гіпобіоз; гіперкапнія; гіпоксія; еритроцит; лімфоцит; тромбоцит; кров; щури; гематологія; лейкоцит; Biology (General); QH301-705.5; Carbonic acid
• Online Access: https://doi.org/10.15587/2519-8025.2017.118786; https://doaj.org/article/8975218040b84fd9952b5a2e286f34b1

The use of hypobiotic state in the medical practice is rather urgent in the modern world. Three most important conditions of creation of artificial hypobiosis are: hypoxia, hypothermia and hypercapnia. The most important parameters that characterize homeostasis of the organism at artificially created conditions of hypobiosis are hematological indices. White non-pedigreed male rats with the mass 180–200 g, kept in standard vivarium conditions, were used for the experiment. Animals were divided in groups: control (intact animals) and experimental ones – the first group (artificial hypobiosis), the second group in 24 hours after going out from artificial hypobiosis. The number of animals in each group n=5. Experiments were realized according to requirements of the “European convention about protection of vertebral animals, used for experimental and other scientific needs” (Strasburg, France, 1985), general ethic principles of experiments on animals, accepted by the First national congress of Ukraine on bioethics (2001). As a result of realized studies there was demonstrated the essential increase of erythrocytes and platelets together with the decrease of the leycocytes content. Under conditions of hypoxia and hypercapnia the growth of the erythrocytes level can be explained by the increase of the carbonic acid level, and the platelets level grows because of the decrease of blood fluidity at artificial hypobiosis