Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium.
Trichomonas vaginalis is a common protozoan parasite, which causes trichomoniasis associated with severe adverse reproductive outcomes. However, the underlying pathogenesis has not been fully understood. As the first line of defense against invading pathogens, the vaginal epithelial cells are highly...
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ftdoajarticles:oai:doaj.org/article:8952476d6eb6492185698720cf31686e 2023-05-15T15:12:39+02:00 Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium. Jian-Bang Xu Shen-Jiao Lu Li-Jiao Ke Zhuo-Er Qiu Lei Chen Hao-Li Zhang Xi-Yuan Wang Xiao-Fan Wei Shuming He Yun-Xin Zhu Zhao-Rong Lun Wen-Liang Zhou Yi-Lin Zhang 2021-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009319 https://doaj.org/article/8952476d6eb6492185698720cf31686e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009319 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009319 https://doaj.org/article/8952476d6eb6492185698720cf31686e PLoS Neglected Tropical Diseases, Vol 15, Iss 4, p e0009319 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009319 2022-12-31T09:21:49Z Trichomonas vaginalis is a common protozoan parasite, which causes trichomoniasis associated with severe adverse reproductive outcomes. However, the underlying pathogenesis has not been fully understood. As the first line of defense against invading pathogens, the vaginal epithelial cells are highly responsive to environmental stimuli and contribute to the formation of the optimal luminal fluid microenvironment. The cystic fibrosis transmembrane conductance regulator (CFTR), an anion channel widely distributed at the apical membrane of epithelial cells, plays a crucial role in mediating the secretion of Cl- and HCO3-. In this study, we investigated the effect of T. vaginalis on vaginal epithelial ion transport elicited by prostaglandin E2 (PGE2), a major prostaglandin in the semen. Luminal administration of PGE2 triggered a remarkable and sustained increase of short-circuit current (ISC) in rat vaginal epithelium, which was mainly due to Cl- and HCO3- secretion mediated by the cAMP-activated CFTR. However, T. vaginalis infection significantly abrogated the ISC response evoked by PGE2, indicating impaired transepithelial anion transport via CFTR. Using a primary cell culture system of rat vaginal epithelium and a human vaginal epithelial cell line, we demonstrated that the expression of CFTR was significantly down-regulated after T. vaginalis infection. In addition, defective Cl- transport function of CFTR was observed in T. vaginalis-infected cells by measuring intracellular Cl- signals. Conclusively, T. vaginalis restrained exogenous PGE2-induced anion secretion through down-regulation of CFTR in vaginal epithelium. These results provide novel insights into the intervention of reproductive complications associated with T. vaginalis infection such as infertility and disequilibrium in vaginal fluid microenvironment. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 4 e0009319 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jian-Bang Xu Shen-Jiao Lu Li-Jiao Ke Zhuo-Er Qiu Lei Chen Hao-Li Zhang Xi-Yuan Wang Xiao-Fan Wei Shuming He Yun-Xin Zhu Zhao-Rong Lun Wen-Liang Zhou Yi-Lin Zhang Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Trichomonas vaginalis is a common protozoan parasite, which causes trichomoniasis associated with severe adverse reproductive outcomes. However, the underlying pathogenesis has not been fully understood. As the first line of defense against invading pathogens, the vaginal epithelial cells are highly responsive to environmental stimuli and contribute to the formation of the optimal luminal fluid microenvironment. The cystic fibrosis transmembrane conductance regulator (CFTR), an anion channel widely distributed at the apical membrane of epithelial cells, plays a crucial role in mediating the secretion of Cl- and HCO3-. In this study, we investigated the effect of T. vaginalis on vaginal epithelial ion transport elicited by prostaglandin E2 (PGE2), a major prostaglandin in the semen. Luminal administration of PGE2 triggered a remarkable and sustained increase of short-circuit current (ISC) in rat vaginal epithelium, which was mainly due to Cl- and HCO3- secretion mediated by the cAMP-activated CFTR. However, T. vaginalis infection significantly abrogated the ISC response evoked by PGE2, indicating impaired transepithelial anion transport via CFTR. Using a primary cell culture system of rat vaginal epithelium and a human vaginal epithelial cell line, we demonstrated that the expression of CFTR was significantly down-regulated after T. vaginalis infection. In addition, defective Cl- transport function of CFTR was observed in T. vaginalis-infected cells by measuring intracellular Cl- signals. Conclusively, T. vaginalis restrained exogenous PGE2-induced anion secretion through down-regulation of CFTR in vaginal epithelium. These results provide novel insights into the intervention of reproductive complications associated with T. vaginalis infection such as infertility and disequilibrium in vaginal fluid microenvironment. |
format |
Article in Journal/Newspaper |
author |
Jian-Bang Xu Shen-Jiao Lu Li-Jiao Ke Zhuo-Er Qiu Lei Chen Hao-Li Zhang Xi-Yuan Wang Xiao-Fan Wei Shuming He Yun-Xin Zhu Zhao-Rong Lun Wen-Liang Zhou Yi-Lin Zhang |
author_facet |
Jian-Bang Xu Shen-Jiao Lu Li-Jiao Ke Zhuo-Er Qiu Lei Chen Hao-Li Zhang Xi-Yuan Wang Xiao-Fan Wei Shuming He Yun-Xin Zhu Zhao-Rong Lun Wen-Liang Zhou Yi-Lin Zhang |
author_sort |
Jian-Bang Xu |
title |
Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium. |
title_short |
Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium. |
title_full |
Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium. |
title_fullStr |
Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium. |
title_full_unstemmed |
Trichomonas vaginalis infection impairs anion secretion in vaginal epithelium. |
title_sort |
trichomonas vaginalis infection impairs anion secretion in vaginal epithelium. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009319 https://doaj.org/article/8952476d6eb6492185698720cf31686e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 4, p e0009319 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009319 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009319 https://doaj.org/article/8952476d6eb6492185698720cf31686e |
op_doi |
https://doi.org/10.1371/journal.pntd.0009319 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
15 |
container_issue |
4 |
container_start_page |
e0009319 |
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1766343307926962176 |