The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds
In the current pilot study, we propose the use of quantitative metabolomics to reconstruct the phylogeny of vertebrates, namely birds. We determined the concentrations of the 67 most abundant metabolites in the eye lenses of the following 14 species from 6 orders of the class Aves (Birds): the Black...
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ftdoajarticles:oai:doaj.org/article:8939fc937b8b454cad61a40c3e2ad0e2 2023-10-01T03:59:41+02:00 The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds Ekaterina A. Zelentsova Lyudmila V. Yanshole Yuri P. Tsentalovich Kirill A. Sharshov Vadim V. Yanshole 2022-07-01T00:00:00Z https://doi.org/10.3390/biology11071089 https://doaj.org/article/8939fc937b8b454cad61a40c3e2ad0e2 EN eng MDPI AG https://www.mdpi.com/2079-7737/11/7/1089 https://doaj.org/toc/2079-7737 doi:10.3390/biology11071089 2079-7737 https://doaj.org/article/8939fc937b8b454cad61a40c3e2ad0e2 Biology, Vol 11, Iss 1089, p 1089 (2022) quantitative metabolomics phylogeny hierarchical clustering analysis NMR spectroscopy eye lens birds Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/biology11071089 2023-09-03T00:41:21Z In the current pilot study, we propose the use of quantitative metabolomics to reconstruct the phylogeny of vertebrates, namely birds. We determined the concentrations of the 67 most abundant metabolites in the eye lenses of the following 14 species from 6 orders of the class Aves (Birds): the Black kite ( Milvus migrans ), Eurasian magpie ( Pica pica ), Northern raven ( Corvus corax ), Eurasian coot ( Fulica atra ), Godlewski’s bunting ( Emberiza godlewskii ), Great crested grebe ( Podiceps cristatus ), Great tit ( Parus major ), Hawfinch ( Coccothraustes coccothraustes ), Hooded crow ( Corvus cornix ), House sparrow ( Passer domesticus ), Rock dove ( Columba livia ), Rook ( Corvus frugilegus ), Short-eared owl ( Asio flammeus ) and Ural owl ( Strix uralensis ). Further analysis shows that the statistical approaches generally used in metabolomics can be applied for differentiation between species, and the most fruitful results were obtained with hierarchical clustering analysis (HCA). We observed the grouping of conspecific samples independently of the sampling place and date. The HCA tree structure supports the key role of genomics in the formation of the lens metabolome, but it also indicates the influence of the species lifestyle. A combination of genomics-based and metabolomics-based phylogeny could potentially resolve arising issues and yield a more reliable tree of life. Article in Journal/Newspaper Strix uralensis Ural Owl Directory of Open Access Journals: DOAJ Articles Parus ENVELOPE(3.950,3.950,-71.983,-71.983) Biology 11 7 1089 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
quantitative metabolomics phylogeny hierarchical clustering analysis NMR spectroscopy eye lens birds Biology (General) QH301-705.5 |
spellingShingle |
quantitative metabolomics phylogeny hierarchical clustering analysis NMR spectroscopy eye lens birds Biology (General) QH301-705.5 Ekaterina A. Zelentsova Lyudmila V. Yanshole Yuri P. Tsentalovich Kirill A. Sharshov Vadim V. Yanshole The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds |
topic_facet |
quantitative metabolomics phylogeny hierarchical clustering analysis NMR spectroscopy eye lens birds Biology (General) QH301-705.5 |
description |
In the current pilot study, we propose the use of quantitative metabolomics to reconstruct the phylogeny of vertebrates, namely birds. We determined the concentrations of the 67 most abundant metabolites in the eye lenses of the following 14 species from 6 orders of the class Aves (Birds): the Black kite ( Milvus migrans ), Eurasian magpie ( Pica pica ), Northern raven ( Corvus corax ), Eurasian coot ( Fulica atra ), Godlewski’s bunting ( Emberiza godlewskii ), Great crested grebe ( Podiceps cristatus ), Great tit ( Parus major ), Hawfinch ( Coccothraustes coccothraustes ), Hooded crow ( Corvus cornix ), House sparrow ( Passer domesticus ), Rock dove ( Columba livia ), Rook ( Corvus frugilegus ), Short-eared owl ( Asio flammeus ) and Ural owl ( Strix uralensis ). Further analysis shows that the statistical approaches generally used in metabolomics can be applied for differentiation between species, and the most fruitful results were obtained with hierarchical clustering analysis (HCA). We observed the grouping of conspecific samples independently of the sampling place and date. The HCA tree structure supports the key role of genomics in the formation of the lens metabolome, but it also indicates the influence of the species lifestyle. A combination of genomics-based and metabolomics-based phylogeny could potentially resolve arising issues and yield a more reliable tree of life. |
format |
Article in Journal/Newspaper |
author |
Ekaterina A. Zelentsova Lyudmila V. Yanshole Yuri P. Tsentalovich Kirill A. Sharshov Vadim V. Yanshole |
author_facet |
Ekaterina A. Zelentsova Lyudmila V. Yanshole Yuri P. Tsentalovich Kirill A. Sharshov Vadim V. Yanshole |
author_sort |
Ekaterina A. Zelentsova |
title |
The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds |
title_short |
The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds |
title_full |
The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds |
title_fullStr |
The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds |
title_full_unstemmed |
The Application of Quantitative Metabolomics for the Taxonomic Differentiation of Birds |
title_sort |
application of quantitative metabolomics for the taxonomic differentiation of birds |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/biology11071089 https://doaj.org/article/8939fc937b8b454cad61a40c3e2ad0e2 |
long_lat |
ENVELOPE(3.950,3.950,-71.983,-71.983) |
geographic |
Parus |
geographic_facet |
Parus |
genre |
Strix uralensis Ural Owl |
genre_facet |
Strix uralensis Ural Owl |
op_source |
Biology, Vol 11, Iss 1089, p 1089 (2022) |
op_relation |
https://www.mdpi.com/2079-7737/11/7/1089 https://doaj.org/toc/2079-7737 doi:10.3390/biology11071089 2079-7737 https://doaj.org/article/8939fc937b8b454cad61a40c3e2ad0e2 |
op_doi |
https://doi.org/10.3390/biology11071089 |
container_title |
Biology |
container_volume |
11 |
container_issue |
7 |
container_start_page |
1089 |
_version_ |
1778534007778050048 |