Comparative Lipidomics Profiling of Acylglycerol from Tuna Oil Selectively Hydrolyzed by Thermomyces Lanuginosus Lipase and Candida Antarctica Lipase A
Lipase hydrolysis is an effective method to develop different functional types of lipids. In this study, tuna oil was partially hydrolyzed at 30% and 60% by Thermomyces lanuginosus lipase (TL 100 L) and Candida Antarctica lipase A (ADL), respectively, to obtain lipid-modified acylglycerols. The lipi...
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ftdoajarticles:oai:doaj.org/article:88c3f000b5754c6ebcb74a17e8a5f84a 2023-05-15T13:41:07+02:00 Comparative Lipidomics Profiling of Acylglycerol from Tuna Oil Selectively Hydrolyzed by Thermomyces Lanuginosus Lipase and Candida Antarctica Lipase A Junyong Xuan Zefu Wang Qiuyu Xia Tingyu Luo Qingya Mao Qinxiu Sun Zongyuan Han Yang Liu Shuai Wei Shucheng Liu 2022-11-01T00:00:00Z https://doi.org/10.3390/foods11223664 https://doaj.org/article/88c3f000b5754c6ebcb74a17e8a5f84a EN eng MDPI AG https://www.mdpi.com/2304-8158/11/22/3664 https://doaj.org/toc/2304-8158 doi:10.3390/foods11223664 2304-8158 https://doaj.org/article/88c3f000b5754c6ebcb74a17e8a5f84a Foods, Vol 11, Iss 3664, p 3664 (2022) tuna oil hydrolyzed acylglycerol selective lipase lipid modification acylglycerol composition Chemical technology TP1-1185 article 2022 ftdoajarticles https://doi.org/10.3390/foods11223664 2022-12-30T22:37:16Z Lipase hydrolysis is an effective method to develop different functional types of lipids. In this study, tuna oil was partially hydrolyzed at 30% and 60% by Thermomyces lanuginosus lipase (TL 100 L) and Candida Antarctica lipase A (ADL), respectively, to obtain lipid-modified acylglycerols. The lipidomic profiling of the acylglycerols was investigated by UPLC-Q-TOF-MS and GC–MS to clarify the lipid modification effect of these two lipases on tuna oil. The results showed that 247 kinds of acylglycerols and 23 kinds of fatty acids were identified in the five samples. In the ADL group, the content of triacylglycerols (TAG) and diacylglycerols (DAG) increased by 4.93% and 114.38%, respectively, with an increase in the hydrolysis degree (HD), while there was a decreasing trend in the TL 100 L group. TL 100 L had a better enrichment effect on DHA, while ADL was more inclined to enrich EPA and hydrolyze saturated fatty acids. Cluster analysis showed that the lipids obtained by the hydrolysis of TL 100 L and ADL were significantly different in the cluster analysis of TAG, DAG, and monoacylglycerols (MAG). TL 100 L has strong TAG selectivity and a strong ability to hydrolyze acylglycerols, while ADL has the potential to synthesize functional lipids containing omega-3 PUFAs, especially DAG. Article in Journal/Newspaper Antarc* Antarctica Directory of Open Access Journals: DOAJ Articles Foods 11 22 3664 |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
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language |
English |
topic |
tuna oil hydrolyzed acylglycerol selective lipase lipid modification acylglycerol composition Chemical technology TP1-1185 |
spellingShingle |
tuna oil hydrolyzed acylglycerol selective lipase lipid modification acylglycerol composition Chemical technology TP1-1185 Junyong Xuan Zefu Wang Qiuyu Xia Tingyu Luo Qingya Mao Qinxiu Sun Zongyuan Han Yang Liu Shuai Wei Shucheng Liu Comparative Lipidomics Profiling of Acylglycerol from Tuna Oil Selectively Hydrolyzed by Thermomyces Lanuginosus Lipase and Candida Antarctica Lipase A |
topic_facet |
tuna oil hydrolyzed acylglycerol selective lipase lipid modification acylglycerol composition Chemical technology TP1-1185 |
description |
Lipase hydrolysis is an effective method to develop different functional types of lipids. In this study, tuna oil was partially hydrolyzed at 30% and 60% by Thermomyces lanuginosus lipase (TL 100 L) and Candida Antarctica lipase A (ADL), respectively, to obtain lipid-modified acylglycerols. The lipidomic profiling of the acylglycerols was investigated by UPLC-Q-TOF-MS and GC–MS to clarify the lipid modification effect of these two lipases on tuna oil. The results showed that 247 kinds of acylglycerols and 23 kinds of fatty acids were identified in the five samples. In the ADL group, the content of triacylglycerols (TAG) and diacylglycerols (DAG) increased by 4.93% and 114.38%, respectively, with an increase in the hydrolysis degree (HD), while there was a decreasing trend in the TL 100 L group. TL 100 L had a better enrichment effect on DHA, while ADL was more inclined to enrich EPA and hydrolyze saturated fatty acids. Cluster analysis showed that the lipids obtained by the hydrolysis of TL 100 L and ADL were significantly different in the cluster analysis of TAG, DAG, and monoacylglycerols (MAG). TL 100 L has strong TAG selectivity and a strong ability to hydrolyze acylglycerols, while ADL has the potential to synthesize functional lipids containing omega-3 PUFAs, especially DAG. |
format |
Article in Journal/Newspaper |
author |
Junyong Xuan Zefu Wang Qiuyu Xia Tingyu Luo Qingya Mao Qinxiu Sun Zongyuan Han Yang Liu Shuai Wei Shucheng Liu |
author_facet |
Junyong Xuan Zefu Wang Qiuyu Xia Tingyu Luo Qingya Mao Qinxiu Sun Zongyuan Han Yang Liu Shuai Wei Shucheng Liu |
author_sort |
Junyong Xuan |
title |
Comparative Lipidomics Profiling of Acylglycerol from Tuna Oil Selectively Hydrolyzed by Thermomyces Lanuginosus Lipase and Candida Antarctica Lipase A |
title_short |
Comparative Lipidomics Profiling of Acylglycerol from Tuna Oil Selectively Hydrolyzed by Thermomyces Lanuginosus Lipase and Candida Antarctica Lipase A |
title_full |
Comparative Lipidomics Profiling of Acylglycerol from Tuna Oil Selectively Hydrolyzed by Thermomyces Lanuginosus Lipase and Candida Antarctica Lipase A |
title_fullStr |
Comparative Lipidomics Profiling of Acylglycerol from Tuna Oil Selectively Hydrolyzed by Thermomyces Lanuginosus Lipase and Candida Antarctica Lipase A |
title_full_unstemmed |
Comparative Lipidomics Profiling of Acylglycerol from Tuna Oil Selectively Hydrolyzed by Thermomyces Lanuginosus Lipase and Candida Antarctica Lipase A |
title_sort |
comparative lipidomics profiling of acylglycerol from tuna oil selectively hydrolyzed by thermomyces lanuginosus lipase and candida antarctica lipase a |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/foods11223664 https://doaj.org/article/88c3f000b5754c6ebcb74a17e8a5f84a |
genre |
Antarc* Antarctica |
genre_facet |
Antarc* Antarctica |
op_source |
Foods, Vol 11, Iss 3664, p 3664 (2022) |
op_relation |
https://www.mdpi.com/2304-8158/11/22/3664 https://doaj.org/toc/2304-8158 doi:10.3390/foods11223664 2304-8158 https://doaj.org/article/88c3f000b5754c6ebcb74a17e8a5f84a |
op_doi |
https://doi.org/10.3390/foods11223664 |
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Foods |
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11 |
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22 |
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3664 |
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