Comparison of molecular methods for Bartonella henselae detection in blood donors.
The Bartonella genus consists of neglected pathogens associated with potentially transfusional-transmitted and fatal human diseases. We aimed to evaluate Bartonella sp. prevalence in 500 blood donors and compare the results with the data already published about these samples. We used molecular diagn...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:8880aaa7826c48d9b91d067cfd7ab36d 2023-07-02T03:31:35+02:00 Comparison of molecular methods for Bartonella henselae detection in blood donors. Marina Rovani Drummond Luciene Silva Dos Santos Amanda Roberta de Almeida Karina de Almeida Lins Maria Lourdes Barjas-Castro Pedro Paulo Vissotto de Paiva Diniz Paulo Eduardo Neves Ferreira Velho 2023-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011336 https://doaj.org/article/8880aaa7826c48d9b91d067cfd7ab36d EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011336 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011336 https://doaj.org/article/8880aaa7826c48d9b91d067cfd7ab36d PLoS Neglected Tropical Diseases, Vol 17, Iss 6, p e0011336 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011336 2023-06-11T00:35:14Z The Bartonella genus consists of neglected pathogens associated with potentially transfusional-transmitted and fatal human diseases. We aimed to evaluate Bartonella sp. prevalence in 500 blood donors and compare the results with the data already published about these samples. We used molecular diagnostic methods to detect Bartonella sp.-DNA from blood and liquid culture samples: (A) conventional PCR for two gene regions, the ITS targeting the genus Bartonella and the specific gltA Bartonella henselae; (B) nested PCR for the ftsZ gene and (C) qualitative real-time PCR for the gltA gene, both B. henselae specific. We obtained 30/500 (6%) DNA detections from the blood samples; 77/500 (15.4%) DNA detections from liquid culture samples and five (1%) samples had DNA detection from both. In total, we detected B. henselae DNA from 102/500 (20.4%) donors. The samples used in this study had already been submitted for Bartonella sp.-DNA detection using only a conventional PCR in liquid culture. Sixteen samples (3.2%) were positive previously, and from these 16 samples, 13 were negative in the new investigation. We concluded that the use of liquid culture combined with different molecular tests increases the possibility of detecting Bartonella sp.-DNA, but the tests do not avoid false-negative results. More than a fifth of blood donors had at least one PCR that detected Bartonella sp.-DNA among the eight molecular reactions performed now (four reactions in whole blood and four in liquid culture). Seven percent had B. henselae-DNA detection for two or more distinct regions. Considering the results obtained previously, the DNA of Bartonella spp. was detected or the agent isolated in 23% of analyzed blood donors. The results establish that the low bacteremia and the fastidious characteristics of the bacterium are challenges to laboratory diagnosis and can make it difficult to confirm the infection in patients with bartonelloses. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 17 6 e0011336 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Marina Rovani Drummond Luciene Silva Dos Santos Amanda Roberta de Almeida Karina de Almeida Lins Maria Lourdes Barjas-Castro Pedro Paulo Vissotto de Paiva Diniz Paulo Eduardo Neves Ferreira Velho Comparison of molecular methods for Bartonella henselae detection in blood donors. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The Bartonella genus consists of neglected pathogens associated with potentially transfusional-transmitted and fatal human diseases. We aimed to evaluate Bartonella sp. prevalence in 500 blood donors and compare the results with the data already published about these samples. We used molecular diagnostic methods to detect Bartonella sp.-DNA from blood and liquid culture samples: (A) conventional PCR for two gene regions, the ITS targeting the genus Bartonella and the specific gltA Bartonella henselae; (B) nested PCR for the ftsZ gene and (C) qualitative real-time PCR for the gltA gene, both B. henselae specific. We obtained 30/500 (6%) DNA detections from the blood samples; 77/500 (15.4%) DNA detections from liquid culture samples and five (1%) samples had DNA detection from both. In total, we detected B. henselae DNA from 102/500 (20.4%) donors. The samples used in this study had already been submitted for Bartonella sp.-DNA detection using only a conventional PCR in liquid culture. Sixteen samples (3.2%) were positive previously, and from these 16 samples, 13 were negative in the new investigation. We concluded that the use of liquid culture combined with different molecular tests increases the possibility of detecting Bartonella sp.-DNA, but the tests do not avoid false-negative results. More than a fifth of blood donors had at least one PCR that detected Bartonella sp.-DNA among the eight molecular reactions performed now (four reactions in whole blood and four in liquid culture). Seven percent had B. henselae-DNA detection for two or more distinct regions. Considering the results obtained previously, the DNA of Bartonella spp. was detected or the agent isolated in 23% of analyzed blood donors. The results establish that the low bacteremia and the fastidious characteristics of the bacterium are challenges to laboratory diagnosis and can make it difficult to confirm the infection in patients with bartonelloses. |
format |
Article in Journal/Newspaper |
author |
Marina Rovani Drummond Luciene Silva Dos Santos Amanda Roberta de Almeida Karina de Almeida Lins Maria Lourdes Barjas-Castro Pedro Paulo Vissotto de Paiva Diniz Paulo Eduardo Neves Ferreira Velho |
author_facet |
Marina Rovani Drummond Luciene Silva Dos Santos Amanda Roberta de Almeida Karina de Almeida Lins Maria Lourdes Barjas-Castro Pedro Paulo Vissotto de Paiva Diniz Paulo Eduardo Neves Ferreira Velho |
author_sort |
Marina Rovani Drummond |
title |
Comparison of molecular methods for Bartonella henselae detection in blood donors. |
title_short |
Comparison of molecular methods for Bartonella henselae detection in blood donors. |
title_full |
Comparison of molecular methods for Bartonella henselae detection in blood donors. |
title_fullStr |
Comparison of molecular methods for Bartonella henselae detection in blood donors. |
title_full_unstemmed |
Comparison of molecular methods for Bartonella henselae detection in blood donors. |
title_sort |
comparison of molecular methods for bartonella henselae detection in blood donors. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011336 https://doaj.org/article/8880aaa7826c48d9b91d067cfd7ab36d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 6, p e0011336 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011336 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011336 https://doaj.org/article/8880aaa7826c48d9b91d067cfd7ab36d |
op_doi |
https://doi.org/10.1371/journal.pntd.0011336 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
6 |
container_start_page |
e0011336 |
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1770270942151311360 |