Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose /
The hydrocarbon-degrading bacterial diversity of the intestinal tract content of fish – the Baltic cod (Gadus morhua), plaice (Platichthys flesus) and the Baltic herring (Clupea harengus) – from the Baltic Sea has been investigated by molecular methods: DNA extraction, amplification polymerase chain...
Published in: | Journal of Environmental Engineering and Landscape Management |
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Vilnius Gediminas Technical University
2011
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Online Access: | https://doi.org/10.3846/16486897.2011.602559 https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2 |
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ftdoajarticles:oai:doaj.org/article:884bb2de2c1740a08105a90a347c68f2 2023-05-15T16:19:05+02:00 Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose / Janina Šyvokienė Liongina Micknienė 2011-10-01T00:00:00Z https://doi.org/10.3846/16486897.2011.602559 https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2 EN eng Vilnius Gediminas Technical University https://tede.vgtu.lt/index.php/JEELM/article/view/5340 https://doaj.org/toc/1648-6897 https://doaj.org/toc/1822-4199 doi:10.3846/16486897.2011.602559 1648-6897 1822-4199 https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2 Journal of Environmental Engineering and Landscape Management, Vol 19, Iss 3 (2011) pollution fish intestinal bacteria abundance of hydrocarbon-degrading bacteria 16S rRNA gene sequencing species composition Environmental engineering TA170-171 article 2011 ftdoajarticles https://doi.org/10.3846/16486897.2011.602559 2022-12-31T11:24:17Z The hydrocarbon-degrading bacterial diversity of the intestinal tract content of fish – the Baltic cod (Gadus morhua), plaice (Platichthys flesus) and the Baltic herring (Clupea harengus) – from the Baltic Sea has been investigated by molecular methods: DNA extraction, amplification polymerase chain reaction product and sequencing of partial 16S rRNA genes. The results of this study show that dense total heterotrophic bacterial populations occur in the intestinal tract of investigated fish. The data obtained showed that the abundance of hydrocarbon-degrading bacteria in the intestinal tract of fish varied from 2.40×104 to 1.08×105 cfu g–1between fish species and was still high. Phenotypic examination of the recorded hydrocarbon-degrading bacteria from the intestinal tract of the Baltic cod, plaice and the Baltic herring revealed that they belong to Aeromonas, Pseudomonas/Shewanella. Molecular species of hydrocarbon-degrading bacteria found in the digestive tract of fish from the Baltic Sea were: Aeromonas veronii, Aeromonas sobria, Shewanella spp. and Acinetobacter spp. We argue that hydrocarbon-degrading bacteria in intestinal tract of fish take part in purification processes, as well as, bacteria in water and play a role in adaptation and survival of fish chronically exposed to pollution with hydrocarbons. Santrauka Angliavandenilius skaidančių bakterijų įvairovė Baltijos jūros žuvų – menkių (Gadus morhua), plekšnių (Platichthys flesus) ir strimėlių (Clupea harengus) žarnynų turinyje tirta molekuliniais metodais: DNR išskyrimas, polimerazės grandininės reakcijos produkto amplifikacija ir 16S rRNR genų sekvenavimas. Tirtų žuvų žarnynuose nustatytos gausios heterotrofinių bakterijų populiacijos. Angliavandenilius skaidančios bakterijos išskirtos iš visų tirtų žuvų žarnynų turinio. Jų kiekis žuvų žarnynuose svyravo nuo 2,4×104 iki 1,08×105 kfv (koloniją formuojantis vienetas) ir buvo pakankamai didelis. Fenotipiniais bakterijų identifikavimo metodais nustatyta, kad angliavandenilius skaidančios bakterijos, ... Article in Journal/Newspaper Gadus morhua Directory of Open Access Journals: DOAJ Articles Kad’ ENVELOPE(40.287,40.287,64.964,64.964) Journal of Environmental Engineering and Landscape Management 19 3 244 250 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
pollution fish intestinal bacteria abundance of hydrocarbon-degrading bacteria 16S rRNA gene sequencing species composition Environmental engineering TA170-171 |
spellingShingle |
pollution fish intestinal bacteria abundance of hydrocarbon-degrading bacteria 16S rRNA gene sequencing species composition Environmental engineering TA170-171 Janina Šyvokienė Liongina Micknienė Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose / |
topic_facet |
pollution fish intestinal bacteria abundance of hydrocarbon-degrading bacteria 16S rRNA gene sequencing species composition Environmental engineering TA170-171 |
description |
The hydrocarbon-degrading bacterial diversity of the intestinal tract content of fish – the Baltic cod (Gadus morhua), plaice (Platichthys flesus) and the Baltic herring (Clupea harengus) – from the Baltic Sea has been investigated by molecular methods: DNA extraction, amplification polymerase chain reaction product and sequencing of partial 16S rRNA genes. The results of this study show that dense total heterotrophic bacterial populations occur in the intestinal tract of investigated fish. The data obtained showed that the abundance of hydrocarbon-degrading bacteria in the intestinal tract of fish varied from 2.40×104 to 1.08×105 cfu g–1between fish species and was still high. Phenotypic examination of the recorded hydrocarbon-degrading bacteria from the intestinal tract of the Baltic cod, plaice and the Baltic herring revealed that they belong to Aeromonas, Pseudomonas/Shewanella. Molecular species of hydrocarbon-degrading bacteria found in the digestive tract of fish from the Baltic Sea were: Aeromonas veronii, Aeromonas sobria, Shewanella spp. and Acinetobacter spp. We argue that hydrocarbon-degrading bacteria in intestinal tract of fish take part in purification processes, as well as, bacteria in water and play a role in adaptation and survival of fish chronically exposed to pollution with hydrocarbons. Santrauka Angliavandenilius skaidančių bakterijų įvairovė Baltijos jūros žuvų – menkių (Gadus morhua), plekšnių (Platichthys flesus) ir strimėlių (Clupea harengus) žarnynų turinyje tirta molekuliniais metodais: DNR išskyrimas, polimerazės grandininės reakcijos produkto amplifikacija ir 16S rRNR genų sekvenavimas. Tirtų žuvų žarnynuose nustatytos gausios heterotrofinių bakterijų populiacijos. Angliavandenilius skaidančios bakterijos išskirtos iš visų tirtų žuvų žarnynų turinio. Jų kiekis žuvų žarnynuose svyravo nuo 2,4×104 iki 1,08×105 kfv (koloniją formuojantis vienetas) ir buvo pakankamai didelis. Fenotipiniais bakterijų identifikavimo metodais nustatyta, kad angliavandenilius skaidančios bakterijos, ... |
format |
Article in Journal/Newspaper |
author |
Janina Šyvokienė Liongina Micknienė |
author_facet |
Janina Šyvokienė Liongina Micknienė |
author_sort |
Janina Šyvokienė |
title |
Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose / |
title_short |
Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose / |
title_full |
Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose / |
title_fullStr |
Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose / |
title_full_unstemmed |
Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose / |
title_sort |
hydrocarbon-degrading bacteria associated with intestinal tract of fish from the baltic sea / angliavandenilius skaidančios bakterijos baltijos jūros žuvų virškinamuosiuose traktuose / |
publisher |
Vilnius Gediminas Technical University |
publishDate |
2011 |
url |
https://doi.org/10.3846/16486897.2011.602559 https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2 |
long_lat |
ENVELOPE(40.287,40.287,64.964,64.964) |
geographic |
Kad’ |
geographic_facet |
Kad’ |
genre |
Gadus morhua |
genre_facet |
Gadus morhua |
op_source |
Journal of Environmental Engineering and Landscape Management, Vol 19, Iss 3 (2011) |
op_relation |
https://tede.vgtu.lt/index.php/JEELM/article/view/5340 https://doaj.org/toc/1648-6897 https://doaj.org/toc/1822-4199 doi:10.3846/16486897.2011.602559 1648-6897 1822-4199 https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2 |
op_doi |
https://doi.org/10.3846/16486897.2011.602559 |
container_title |
Journal of Environmental Engineering and Landscape Management |
container_volume |
19 |
container_issue |
3 |
container_start_page |
244 |
op_container_end_page |
250 |
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1766005403691253760 |