Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose /

The hydrocarbon-degrading bacterial diversity of the intestinal tract content of fish – the Baltic cod (Gadus morhua), plaice (Platichthys flesus) and the Baltic herring (Clupea harengus) – from the Baltic Sea has been investigated by molecular methods: DNA extraction, amplification polymerase chain...

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Published in:Journal of Environmental Engineering and Landscape Management
Main Authors: Janina Šyvokienė, Liongina Micknienė
Format: Article in Journal/Newspaper
Language:English
Published: Vilnius Gediminas Technical University 2011
Subjects:
Online Access:https://doi.org/10.3846/16486897.2011.602559
https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2
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spelling ftdoajarticles:oai:doaj.org/article:884bb2de2c1740a08105a90a347c68f2 2023-05-15T16:19:05+02:00 Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose / Janina Šyvokienė Liongina Micknienė 2011-10-01T00:00:00Z https://doi.org/10.3846/16486897.2011.602559 https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2 EN eng Vilnius Gediminas Technical University https://tede.vgtu.lt/index.php/JEELM/article/view/5340 https://doaj.org/toc/1648-6897 https://doaj.org/toc/1822-4199 doi:10.3846/16486897.2011.602559 1648-6897 1822-4199 https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2 Journal of Environmental Engineering and Landscape Management, Vol 19, Iss 3 (2011) pollution fish intestinal bacteria abundance of hydrocarbon-degrading bacteria 16S rRNA gene sequencing species composition Environmental engineering TA170-171 article 2011 ftdoajarticles https://doi.org/10.3846/16486897.2011.602559 2022-12-31T11:24:17Z The hydrocarbon-degrading bacterial diversity of the intestinal tract content of fish – the Baltic cod (Gadus morhua), plaice (Platichthys flesus) and the Baltic herring (Clupea harengus) – from the Baltic Sea has been investigated by molecular methods: DNA extraction, amplification polymerase chain reaction product and sequencing of partial 16S rRNA genes. The results of this study show that dense total heterotrophic bacterial populations occur in the intestinal tract of investigated fish. The data obtained showed that the abundance of hydrocarbon-degrading bacteria in the intestinal tract of fish varied from 2.40×104 to 1.08×105 cfu g–1between fish species and was still high. Phenotypic examination of the recorded hydrocarbon-degrading bacteria from the intestinal tract of the Baltic cod, plaice and the Baltic herring revealed that they belong to Aeromonas, Pseudomonas/Shewanella. Molecular species of hydrocarbon-degrading bacteria found in the digestive tract of fish from the Baltic Sea were: Aeromonas veronii, Aeromonas sobria, Shewanella spp. and Acinetobacter spp. We argue that hydrocarbon-degrading bacteria in intestinal tract of fish take part in purification processes, as well as, bacteria in water and play a role in adaptation and survival of fish chronically exposed to pollution with hydrocarbons. Santrauka Angliavandenilius skaidančių bakterijų įvairovė Baltijos jūros žuvų – menkių (Gadus morhua), plekšnių (Platichthys flesus) ir strimėlių (Clupea harengus) žarnynų turinyje tirta molekuliniais metodais: DNR išskyrimas, polimerazės grandininės reakcijos produkto amplifikacija ir 16S rRNR genų sekvenavimas. Tirtų žuvų žarnynuose nustatytos gausios heterotrofinių bakterijų populiacijos. Angliavandenilius skaidančios bakterijos išskirtos iš visų tirtų žuvų žarnynų turinio. Jų kiekis žuvų žarnynuose svyravo nuo 2,4×104 iki 1,08×105 kfv (koloniją formuojantis vienetas) ir buvo pakankamai didelis. Fenotipiniais bakterijų identifikavimo metodais nustatyta, kad angliavandenilius skaidančios bakterijos, ... Article in Journal/Newspaper Gadus morhua Directory of Open Access Journals: DOAJ Articles Kad’ ENVELOPE(40.287,40.287,64.964,64.964) Journal of Environmental Engineering and Landscape Management 19 3 244 250
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic pollution
fish
intestinal bacteria
abundance of hydrocarbon-degrading bacteria
16S rRNA gene sequencing
species composition
Environmental engineering
TA170-171
spellingShingle pollution
fish
intestinal bacteria
abundance of hydrocarbon-degrading bacteria
16S rRNA gene sequencing
species composition
Environmental engineering
TA170-171
Janina Šyvokienė
Liongina Micknienė
Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose /
topic_facet pollution
fish
intestinal bacteria
abundance of hydrocarbon-degrading bacteria
16S rRNA gene sequencing
species composition
Environmental engineering
TA170-171
description The hydrocarbon-degrading bacterial diversity of the intestinal tract content of fish – the Baltic cod (Gadus morhua), plaice (Platichthys flesus) and the Baltic herring (Clupea harengus) – from the Baltic Sea has been investigated by molecular methods: DNA extraction, amplification polymerase chain reaction product and sequencing of partial 16S rRNA genes. The results of this study show that dense total heterotrophic bacterial populations occur in the intestinal tract of investigated fish. The data obtained showed that the abundance of hydrocarbon-degrading bacteria in the intestinal tract of fish varied from 2.40×104 to 1.08×105 cfu g–1between fish species and was still high. Phenotypic examination of the recorded hydrocarbon-degrading bacteria from the intestinal tract of the Baltic cod, plaice and the Baltic herring revealed that they belong to Aeromonas, Pseudomonas/Shewanella. Molecular species of hydrocarbon-degrading bacteria found in the digestive tract of fish from the Baltic Sea were: Aeromonas veronii, Aeromonas sobria, Shewanella spp. and Acinetobacter spp. We argue that hydrocarbon-degrading bacteria in intestinal tract of fish take part in purification processes, as well as, bacteria in water and play a role in adaptation and survival of fish chronically exposed to pollution with hydrocarbons. Santrauka Angliavandenilius skaidančių bakterijų įvairovė Baltijos jūros žuvų – menkių (Gadus morhua), plekšnių (Platichthys flesus) ir strimėlių (Clupea harengus) žarnynų turinyje tirta molekuliniais metodais: DNR išskyrimas, polimerazės grandininės reakcijos produkto amplifikacija ir 16S rRNR genų sekvenavimas. Tirtų žuvų žarnynuose nustatytos gausios heterotrofinių bakterijų populiacijos. Angliavandenilius skaidančios bakterijos išskirtos iš visų tirtų žuvų žarnynų turinio. Jų kiekis žuvų žarnynuose svyravo nuo 2,4×104 iki 1,08×105 kfv (koloniją formuojantis vienetas) ir buvo pakankamai didelis. Fenotipiniais bakterijų identifikavimo metodais nustatyta, kad angliavandenilius skaidančios bakterijos, ...
format Article in Journal/Newspaper
author Janina Šyvokienė
Liongina Micknienė
author_facet Janina Šyvokienė
Liongina Micknienė
author_sort Janina Šyvokienė
title Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose /
title_short Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose /
title_full Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose /
title_fullStr Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose /
title_full_unstemmed Hydrocarbon-degrading bacteria associated with intestinal tract of fish from the Baltic sea / Angliavandenilius skaidančios bakterijos Baltijos jūros žuvų virškinamuosiuose traktuose /
title_sort hydrocarbon-degrading bacteria associated with intestinal tract of fish from the baltic sea / angliavandenilius skaidančios bakterijos baltijos jūros žuvų virškinamuosiuose traktuose /
publisher Vilnius Gediminas Technical University
publishDate 2011
url https://doi.org/10.3846/16486897.2011.602559
https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2
long_lat ENVELOPE(40.287,40.287,64.964,64.964)
geographic Kad’
geographic_facet Kad’
genre Gadus morhua
genre_facet Gadus morhua
op_source Journal of Environmental Engineering and Landscape Management, Vol 19, Iss 3 (2011)
op_relation https://tede.vgtu.lt/index.php/JEELM/article/view/5340
https://doaj.org/toc/1648-6897
https://doaj.org/toc/1822-4199
doi:10.3846/16486897.2011.602559
1648-6897
1822-4199
https://doaj.org/article/884bb2de2c1740a08105a90a347c68f2
op_doi https://doi.org/10.3846/16486897.2011.602559
container_title Journal of Environmental Engineering and Landscape Management
container_volume 19
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