Multiple expressed MHC class II loci in salmonids; details of one non-classical region in Atlantic salmon ( Salmo salar )
Abstract Background In teleosts, the Major Histocompatibility Complex (MHC) class I and class II molecules reside on different linkage groups as opposed to tetrapods and shark, where the class I and class II genes reside in one genomic region. Several teleost MHC class I regions have been sequenced...
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ftdoajarticles:oai:doaj.org/article:8845464d458a4cc5b3389dd246b895d2 2023-05-15T15:32:54+02:00 Multiple expressed MHC class II loci in salmonids; details of one non-classical region in Atlantic salmon ( Salmo salar ) Bakke Hege G Lukacs Morten F Harstad Håvard Grimholt Unni 2008-04-01T00:00:00Z https://doi.org/10.1186/1471-2164-9-193 https://doaj.org/article/8845464d458a4cc5b3389dd246b895d2 EN eng BMC http://www.biomedcentral.com/1471-2164/9/193 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-9-193 1471-2164 https://doaj.org/article/8845464d458a4cc5b3389dd246b895d2 BMC Genomics, Vol 9, Iss 1, p 193 (2008) Biotechnology TP248.13-248.65 Genetics QH426-470 article 2008 ftdoajarticles https://doi.org/10.1186/1471-2164-9-193 2023-01-08T01:28:10Z Abstract Background In teleosts, the Major Histocompatibility Complex (MHC) class I and class II molecules reside on different linkage groups as opposed to tetrapods and shark, where the class I and class II genes reside in one genomic region. Several teleost MHC class I regions have been sequenced and show varying number of class I genes. Salmonids have one major expressed MHC class I locus (UBA) in addition to varying numbers of non-classical genes. Two other more distant lineages are also identifyed denoted L and ZE. For class II, only one major expressed class II alpha (DAA) and beta (DAB) gene has been identified in salmonids so far. Results We sequenced a genomic region of 211 kb encompassing divergent MHC class II alpha ( Sasa-DBA ) and beta ( Sasa-DBB ) genes in addition to NRGN, TIPRL, TBCEL and TECTA. The region was not linked to the classical class II genes and had some synteny to genomic regions from other teleosts. Two additional divergent and expressed class II sequences denoted DCA and DDA were also identified in both salmon and trout. Expression patterns and lack of polymorphism make these genes non-classical class II analogues. Sasa-DBB , Sasa-DCA and Sasa-DDA had highest expression levels in liver, hindgut and spleen respectively, suggestive of distinctive functions in these tissues. Phylogenetic studies revealed more yet undescribed divergent expressed MHC class II molecules also in other teleosts. Conclusion We have characterised one genomic region containing expressed non-classical MHC class II genes in addition to four other genes not involved in immune function. Salmonids contain at least two expressed MHC class II beta genes and four expressed MHC class II alpha genes with properties suggestive of new functions for MHC class II in vertebrates. Collectively, our data suggest that the class II is worthy of more elaborate studies also in other teleost species. Article in Journal/Newspaper Atlantic salmon Salmo salar Directory of Open Access Journals: DOAJ Articles BMC Genomics 9 1 193 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
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Biotechnology TP248.13-248.65 Genetics QH426-470 Bakke Hege G Lukacs Morten F Harstad Håvard Grimholt Unni Multiple expressed MHC class II loci in salmonids; details of one non-classical region in Atlantic salmon ( Salmo salar ) |
topic_facet |
Biotechnology TP248.13-248.65 Genetics QH426-470 |
description |
Abstract Background In teleosts, the Major Histocompatibility Complex (MHC) class I and class II molecules reside on different linkage groups as opposed to tetrapods and shark, where the class I and class II genes reside in one genomic region. Several teleost MHC class I regions have been sequenced and show varying number of class I genes. Salmonids have one major expressed MHC class I locus (UBA) in addition to varying numbers of non-classical genes. Two other more distant lineages are also identifyed denoted L and ZE. For class II, only one major expressed class II alpha (DAA) and beta (DAB) gene has been identified in salmonids so far. Results We sequenced a genomic region of 211 kb encompassing divergent MHC class II alpha ( Sasa-DBA ) and beta ( Sasa-DBB ) genes in addition to NRGN, TIPRL, TBCEL and TECTA. The region was not linked to the classical class II genes and had some synteny to genomic regions from other teleosts. Two additional divergent and expressed class II sequences denoted DCA and DDA were also identified in both salmon and trout. Expression patterns and lack of polymorphism make these genes non-classical class II analogues. Sasa-DBB , Sasa-DCA and Sasa-DDA had highest expression levels in liver, hindgut and spleen respectively, suggestive of distinctive functions in these tissues. Phylogenetic studies revealed more yet undescribed divergent expressed MHC class II molecules also in other teleosts. Conclusion We have characterised one genomic region containing expressed non-classical MHC class II genes in addition to four other genes not involved in immune function. Salmonids contain at least two expressed MHC class II beta genes and four expressed MHC class II alpha genes with properties suggestive of new functions for MHC class II in vertebrates. Collectively, our data suggest that the class II is worthy of more elaborate studies also in other teleost species. |
format |
Article in Journal/Newspaper |
author |
Bakke Hege G Lukacs Morten F Harstad Håvard Grimholt Unni |
author_facet |
Bakke Hege G Lukacs Morten F Harstad Håvard Grimholt Unni |
author_sort |
Bakke Hege G |
title |
Multiple expressed MHC class II loci in salmonids; details of one non-classical region in Atlantic salmon ( Salmo salar ) |
title_short |
Multiple expressed MHC class II loci in salmonids; details of one non-classical region in Atlantic salmon ( Salmo salar ) |
title_full |
Multiple expressed MHC class II loci in salmonids; details of one non-classical region in Atlantic salmon ( Salmo salar ) |
title_fullStr |
Multiple expressed MHC class II loci in salmonids; details of one non-classical region in Atlantic salmon ( Salmo salar ) |
title_full_unstemmed |
Multiple expressed MHC class II loci in salmonids; details of one non-classical region in Atlantic salmon ( Salmo salar ) |
title_sort |
multiple expressed mhc class ii loci in salmonids; details of one non-classical region in atlantic salmon ( salmo salar ) |
publisher |
BMC |
publishDate |
2008 |
url |
https://doi.org/10.1186/1471-2164-9-193 https://doaj.org/article/8845464d458a4cc5b3389dd246b895d2 |
genre |
Atlantic salmon Salmo salar |
genre_facet |
Atlantic salmon Salmo salar |
op_source |
BMC Genomics, Vol 9, Iss 1, p 193 (2008) |
op_relation |
http://www.biomedcentral.com/1471-2164/9/193 https://doaj.org/toc/1471-2164 doi:10.1186/1471-2164-9-193 1471-2164 https://doaj.org/article/8845464d458a4cc5b3389dd246b895d2 |
op_doi |
https://doi.org/10.1186/1471-2164-9-193 |
container_title |
BMC Genomics |
container_volume |
9 |
container_issue |
1 |
container_start_page |
193 |
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1766363380973568000 |