Additive protection by antioxidant and apoptosis-inhibiting effects on mosquito cells with dengue 2 virus infection.
Cytopathic effects (CPEs) in mosquito cells are generally trivial compared to those that occur in mammalian cells, which usually end up undergoing apoptosis during dengue virus (DENV) infection. However, oxidative stress was detected in both types of infected cells. Despite this, the survival of mos...
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ftdoajarticles:oai:doaj.org/article:88439687a2b44587b3c75b5f58a67f57 2023-05-15T15:08:38+02:00 Additive protection by antioxidant and apoptosis-inhibiting effects on mosquito cells with dengue 2 virus infection. Tien-Huang Chen Yin-Ping Lo Chao-Fu Yang Wei-June Chen 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001613 https://doaj.org/article/88439687a2b44587b3c75b5f58a67f57 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3328429?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001613 https://doaj.org/article/88439687a2b44587b3c75b5f58a67f57 PLoS Neglected Tropical Diseases, Vol 6, Iss 4, p e1613 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001613 2022-12-31T03:22:24Z Cytopathic effects (CPEs) in mosquito cells are generally trivial compared to those that occur in mammalian cells, which usually end up undergoing apoptosis during dengue virus (DENV) infection. However, oxidative stress was detected in both types of infected cells. Despite this, the survival of mosquito cells benefits from the upregulation of genes related to antioxidant defense, such as glutathione S transferase (GST). A second defense system, i.e., consisting of antiapoptotic effects, was also shown to play a role in protecting mosquito cells against DENV infection. This system is regulated by an inhibitor of apoptosis (IAP) that is an upstream regulator of caspases-9 and -3. DENV-infected C6/36 cells with double knockdown of GST and the IAP showed a synergistic effect on activation of these two caspases, causing a higher rate of apoptosis (> 20%) than those with knockdown of each single gene (-10%). It seems that the IAP acts as a second line of defense with an additional effect on the survival of mosquito cells with DENV infection. Compared to mammalian cells, residual hydrogen peroxide in DENV-infected C6/36 cells may signal for upregulation of the IAP. This novel finding sheds light on virus/cell interactions and their coevolution that may elucidate how mosquitoes can be a vector of DENV and probably most other arboviruses in nature. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 4 e1613 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Tien-Huang Chen Yin-Ping Lo Chao-Fu Yang Wei-June Chen Additive protection by antioxidant and apoptosis-inhibiting effects on mosquito cells with dengue 2 virus infection. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Cytopathic effects (CPEs) in mosquito cells are generally trivial compared to those that occur in mammalian cells, which usually end up undergoing apoptosis during dengue virus (DENV) infection. However, oxidative stress was detected in both types of infected cells. Despite this, the survival of mosquito cells benefits from the upregulation of genes related to antioxidant defense, such as glutathione S transferase (GST). A second defense system, i.e., consisting of antiapoptotic effects, was also shown to play a role in protecting mosquito cells against DENV infection. This system is regulated by an inhibitor of apoptosis (IAP) that is an upstream regulator of caspases-9 and -3. DENV-infected C6/36 cells with double knockdown of GST and the IAP showed a synergistic effect on activation of these two caspases, causing a higher rate of apoptosis (> 20%) than those with knockdown of each single gene (-10%). It seems that the IAP acts as a second line of defense with an additional effect on the survival of mosquito cells with DENV infection. Compared to mammalian cells, residual hydrogen peroxide in DENV-infected C6/36 cells may signal for upregulation of the IAP. This novel finding sheds light on virus/cell interactions and their coevolution that may elucidate how mosquitoes can be a vector of DENV and probably most other arboviruses in nature. |
format |
Article in Journal/Newspaper |
author |
Tien-Huang Chen Yin-Ping Lo Chao-Fu Yang Wei-June Chen |
author_facet |
Tien-Huang Chen Yin-Ping Lo Chao-Fu Yang Wei-June Chen |
author_sort |
Tien-Huang Chen |
title |
Additive protection by antioxidant and apoptosis-inhibiting effects on mosquito cells with dengue 2 virus infection. |
title_short |
Additive protection by antioxidant and apoptosis-inhibiting effects on mosquito cells with dengue 2 virus infection. |
title_full |
Additive protection by antioxidant and apoptosis-inhibiting effects on mosquito cells with dengue 2 virus infection. |
title_fullStr |
Additive protection by antioxidant and apoptosis-inhibiting effects on mosquito cells with dengue 2 virus infection. |
title_full_unstemmed |
Additive protection by antioxidant and apoptosis-inhibiting effects on mosquito cells with dengue 2 virus infection. |
title_sort |
additive protection by antioxidant and apoptosis-inhibiting effects on mosquito cells with dengue 2 virus infection. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001613 https://doaj.org/article/88439687a2b44587b3c75b5f58a67f57 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 4, p e1613 (2012) |
op_relation |
http://europepmc.org/articles/PMC3328429?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001613 https://doaj.org/article/88439687a2b44587b3c75b5f58a67f57 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001613 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
6 |
container_issue |
4 |
container_start_page |
e1613 |
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1766339950414921728 |