Molecular epidemiology of Klebsiella pneumoniae resistant to beta-lactams antibiotics isolated from health centers of Aragua State-Venezuela
K. pneumoniae resistance to β-lactam antibiotics is a public health problem. The objective was to characterize by molecular epidemiology isolates of K. pneumoniae resistant to β-lactams in four Health Centers of the Aragua State and establish the association between genotypes with resistance and epi...
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Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales
2020
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ftdoajarticles:oai:doaj.org/article:88228df8b51c42ef88908c68242c0e0e 2023-10-09T21:49:06+02:00 Molecular epidemiology of Klebsiella pneumoniae resistant to beta-lactams antibiotics isolated from health centers of Aragua State-Venezuela Lisbeth Carolina Sierra Ysvette Vasquez Luis Perez-Ybarra María Victoria Mendez López 2020-10-01T00:00:00Z https://doi.org/10.5281/zenodo.4081865 https://doaj.org/article/88228df8b51c42ef88908c68242c0e0e EN ES eng spa Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales https://produccioncientificaluz.org/index.php/kasmera/article/view/32378 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 doi:10.5281/zenodo.4081865 https://doaj.org/article/88228df8b51c42ef88908c68242c0e0e Kasmera, Vol 48, Iss 2, Pp e48232378-e48232378 (2020) klebsiella pneumoniae epidemiología molecular resistencia antibiótica beta-lactamasas Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.5281/zenodo.4081865 2023-09-10T00:44:41Z K. pneumoniae resistance to β-lactam antibiotics is a public health problem. The objective was to characterize by molecular epidemiology isolates of K. pneumoniae resistant to β-lactams in four Health Centers of the Aragua State and establish the association between genotypes with resistance and epidemiological variables. 72 strains of K. pneumoniae were processed and their resistance to β-lactams was performed according to the CLSI guidelines. Double disc synergy was used for phenotypic detection of Extended Spectrum β-lactamase or ESBL. Combinations of EDTA/imipenem/meropenem; phenylboronic acid/meropenem/imipenem and piperacillin tazobactam/ ceftazidime/Imipenem/cefoxitin were used to detect metallo-beta-lactamase or MBLs, carbapenemases (KPC) and inducible AmpC respectively. Molecular typing was performed by polymerase chain reaction of palindromic extragenic repetitive sequences. Only 35 strains (48.6%) were resistant to all β-lactams. 34.29%; 31.43% and 31.43% turned out to be ESBL, KPC and MBLs respectively, and 2.86% inducible AmpC. Seven genotypes were identified, where type B grouped 23 genetically identical strains and clonally spreaded. A statistically significant relationship was found between genotype, age and gender. In conclusion, K. pneumoniae is highly resistant to β-lactams. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
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English Spanish |
topic |
klebsiella pneumoniae epidemiología molecular resistencia antibiótica beta-lactamasas Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
klebsiella pneumoniae epidemiología molecular resistencia antibiótica beta-lactamasas Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Lisbeth Carolina Sierra Ysvette Vasquez Luis Perez-Ybarra María Victoria Mendez López Molecular epidemiology of Klebsiella pneumoniae resistant to beta-lactams antibiotics isolated from health centers of Aragua State-Venezuela |
topic_facet |
klebsiella pneumoniae epidemiología molecular resistencia antibiótica beta-lactamasas Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
K. pneumoniae resistance to β-lactam antibiotics is a public health problem. The objective was to characterize by molecular epidemiology isolates of K. pneumoniae resistant to β-lactams in four Health Centers of the Aragua State and establish the association between genotypes with resistance and epidemiological variables. 72 strains of K. pneumoniae were processed and their resistance to β-lactams was performed according to the CLSI guidelines. Double disc synergy was used for phenotypic detection of Extended Spectrum β-lactamase or ESBL. Combinations of EDTA/imipenem/meropenem; phenylboronic acid/meropenem/imipenem and piperacillin tazobactam/ ceftazidime/Imipenem/cefoxitin were used to detect metallo-beta-lactamase or MBLs, carbapenemases (KPC) and inducible AmpC respectively. Molecular typing was performed by polymerase chain reaction of palindromic extragenic repetitive sequences. Only 35 strains (48.6%) were resistant to all β-lactams. 34.29%; 31.43% and 31.43% turned out to be ESBL, KPC and MBLs respectively, and 2.86% inducible AmpC. Seven genotypes were identified, where type B grouped 23 genetically identical strains and clonally spreaded. A statistically significant relationship was found between genotype, age and gender. In conclusion, K. pneumoniae is highly resistant to β-lactams. |
format |
Article in Journal/Newspaper |
author |
Lisbeth Carolina Sierra Ysvette Vasquez Luis Perez-Ybarra María Victoria Mendez López |
author_facet |
Lisbeth Carolina Sierra Ysvette Vasquez Luis Perez-Ybarra María Victoria Mendez López |
author_sort |
Lisbeth Carolina Sierra |
title |
Molecular epidemiology of Klebsiella pneumoniae resistant to beta-lactams antibiotics isolated from health centers of Aragua State-Venezuela |
title_short |
Molecular epidemiology of Klebsiella pneumoniae resistant to beta-lactams antibiotics isolated from health centers of Aragua State-Venezuela |
title_full |
Molecular epidemiology of Klebsiella pneumoniae resistant to beta-lactams antibiotics isolated from health centers of Aragua State-Venezuela |
title_fullStr |
Molecular epidemiology of Klebsiella pneumoniae resistant to beta-lactams antibiotics isolated from health centers of Aragua State-Venezuela |
title_full_unstemmed |
Molecular epidemiology of Klebsiella pneumoniae resistant to beta-lactams antibiotics isolated from health centers of Aragua State-Venezuela |
title_sort |
molecular epidemiology of klebsiella pneumoniae resistant to beta-lactams antibiotics isolated from health centers of aragua state-venezuela |
publisher |
Universidad del Zulia,Facultad de Medicina,Departamento de Enfermedades Infecciosas y Tropicales |
publishDate |
2020 |
url |
https://doi.org/10.5281/zenodo.4081865 https://doaj.org/article/88228df8b51c42ef88908c68242c0e0e |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Kasmera, Vol 48, Iss 2, Pp e48232378-e48232378 (2020) |
op_relation |
https://produccioncientificaluz.org/index.php/kasmera/article/view/32378 https://doaj.org/toc/0075-5222 https://doaj.org/toc/2477-9628 0075-5222 2477-9628 doi:10.5281/zenodo.4081865 https://doaj.org/article/88228df8b51c42ef88908c68242c0e0e |
op_doi |
https://doi.org/10.5281/zenodo.4081865 |
_version_ |
1779312133760090112 |