Genomics of Tenacibaculum Species in British Columbia, Canada

Tenacibaculum is a genus of Gram-negative filamentous bacteria with a cosmopolitan distribution. The research describing Tenacibaculum genomes stems primarily from Norway and Chile due to their impacts on salmon aquaculture. Canadian salmon aquaculture also experiences mortality events related to th...

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Bibliographic Details
Published in:Pathogens
Main Authors: Joseph P. Nowlan, Ashton N. Sies, Scott R. Britney, Andrew D. S. Cameron, Ahmed Siah, John S. Lumsden, Spencer Russell
Format: Article in Journal/Newspaper
Language:English
Published: MDPI AG 2023
Subjects:
phylogenetics
de novo assembly
virulence
antimicrobial resistance
diversity
Tenacibaculum
Medicine
R
Canada
Norway
British Columbia
Atlantic salmon
Online Access:https://doi.org/10.3390/pathogens12010101
https://doaj.org/article/87fb02e29b134d5397a2e76c9e566561
Description
Summary:Tenacibaculum is a genus of Gram-negative filamentous bacteria with a cosmopolitan distribution. The research describing Tenacibaculum genomes stems primarily from Norway and Chile due to their impacts on salmon aquaculture. Canadian salmon aquaculture also experiences mortality events related to the presence of Tenacibaculum spp., yet no Canadian Tenacibaculum genomes are publicly available. Ribosomal DNA sequencing of 16S and four species-specific 16S quantitative-PCR assays were used to select isolates cultured from Atlantic salmon with mouthrot in British Columbia (BC), Canada. Ten isolates representing four known and two unknown species of Tenacibaculum were selected for shotgun whole genome sequencing using the Oxford Nanopore’s MinION platform. The genome assemblies achieved closed circular chromosomes for seven isolates and long contigs for the remaining three isolates. Average nucleotide identity analysis identified T. ovolyticum , T. maritimum , T. dicentrarchi , two genomovars of T. finnmarkense , and two proposed novel species T. pacificus sp. nov. type strain 18-2881-A T and T. retecalamus sp. nov. type strain 18-3228-7B T . Annotation in most of the isolates predicted putative virulence and antimicrobial resistance genes, most-notably toxins (i.e., hemolysins), type-IX secretion systems, and oxytetracycline resistance. Comparative analysis with the T. maritimum type-strain predicted additional toxins and numerous C-terminal secretion proteins, including an M12B family metalloprotease in the T. maritimum isolates from BC. The genomic prediction of virulence-associated genes provides important targets for studies of mouthrot disease, and the annotation of the antimicrobial resistance genes provides targets for surveillance and diagnosis in veterinary medicine.

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