Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes
Abstract Background The intra-erythrocytic development of the malaria parasite Plasmodium falciparum depends on the uptake of a number of essential nutrients from the host cell and blood plasma. It is widely recognized that the parasite imports low molecular weight solutes from the plasma and the co...
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ftdoajarticles:oai:doaj.org/article:87efd33c13dc411baf4741971195b93b 2023-05-15T15:13:48+02:00 Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes Sarah El Chamy Maluf Marcelo Yudi Icimoto Pollyana Maria Saud Melo Alexandre Budu Rita Coimbra Marcos Leoni Gazarini Adriana Karaoglanovic Carmona 2020-08-01T00:00:00Z https://doi.org/10.1186/s12936-020-03377-4 https://doaj.org/article/87efd33c13dc411baf4741971195b93b EN eng BMC http://link.springer.com/article/10.1186/s12936-020-03377-4 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03377-4 1475-2875 https://doaj.org/article/87efd33c13dc411baf4741971195b93b Malaria Journal, Vol 19, Iss 1, Pp 1-10 (2020) Plasmodium falciparum Protein uptake Plasminogen Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2020 ftdoajarticles https://doi.org/10.1186/s12936-020-03377-4 2022-12-31T03:05:19Z Abstract Background The intra-erythrocytic development of the malaria parasite Plasmodium falciparum depends on the uptake of a number of essential nutrients from the host cell and blood plasma. It is widely recognized that the parasite imports low molecular weight solutes from the plasma and the consumption of these nutrients by P. falciparum has been extensively analysed. However, although it was already shown that the parasite also imports functional proteins from the vertebrate host, the internalization route through the different infected erythrocyte membranes has not yet been elucidated. In order to further understand the uptake mechanism, the study examined the trafficking of human plasminogen from the extracellular medium into P. falciparum-infected red blood cells. Methods Plasmodium falciparum clone 3D7 was cultured in standard HEPES-buffered RPMI 1640 medium supplemented with 0.5% AlbuMAX. Exogenous human plasminogen was added to the P. falciparum culture and the uptake of this protein by the parasites was analysed by electron microscopy and Western blotting. Immunoprecipitation and mass spectrometry were performed to investigate possible protein interactions that may assist plasminogen import into infected erythrocytes. The effect of pharmacological inhibitors of different cellular physiological processes in plasminogen uptake was also tested. Results It was observed that plasminogen was selectively internalized by P. falciparum-infected erythrocytes, with localization in plasma membrane erythrocyte and parasite’s cytosol. The protein was not detected in parasitic food vacuole and haemoglobin-containing vesicles. Furthermore, in erythrocyte cytoplasm, plasminogen was associated with the parasite-derived membranous structures tubovesicular network (TVN) and Maurer’s clefts. Several proteins were identified in immunoprecipitation assay and may be involved in the delivery of plasminogen across the P. falciparum multiple compartments. Conclusion The findings here reported reveal new features regarding ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 19 1 |
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English |
topic |
Plasmodium falciparum Protein uptake Plasminogen Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium falciparum Protein uptake Plasminogen Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Sarah El Chamy Maluf Marcelo Yudi Icimoto Pollyana Maria Saud Melo Alexandre Budu Rita Coimbra Marcos Leoni Gazarini Adriana Karaoglanovic Carmona Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes |
topic_facet |
Plasmodium falciparum Protein uptake Plasminogen Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The intra-erythrocytic development of the malaria parasite Plasmodium falciparum depends on the uptake of a number of essential nutrients from the host cell and blood plasma. It is widely recognized that the parasite imports low molecular weight solutes from the plasma and the consumption of these nutrients by P. falciparum has been extensively analysed. However, although it was already shown that the parasite also imports functional proteins from the vertebrate host, the internalization route through the different infected erythrocyte membranes has not yet been elucidated. In order to further understand the uptake mechanism, the study examined the trafficking of human plasminogen from the extracellular medium into P. falciparum-infected red blood cells. Methods Plasmodium falciparum clone 3D7 was cultured in standard HEPES-buffered RPMI 1640 medium supplemented with 0.5% AlbuMAX. Exogenous human plasminogen was added to the P. falciparum culture and the uptake of this protein by the parasites was analysed by electron microscopy and Western blotting. Immunoprecipitation and mass spectrometry were performed to investigate possible protein interactions that may assist plasminogen import into infected erythrocytes. The effect of pharmacological inhibitors of different cellular physiological processes in plasminogen uptake was also tested. Results It was observed that plasminogen was selectively internalized by P. falciparum-infected erythrocytes, with localization in plasma membrane erythrocyte and parasite’s cytosol. The protein was not detected in parasitic food vacuole and haemoglobin-containing vesicles. Furthermore, in erythrocyte cytoplasm, plasminogen was associated with the parasite-derived membranous structures tubovesicular network (TVN) and Maurer’s clefts. Several proteins were identified in immunoprecipitation assay and may be involved in the delivery of plasminogen across the P. falciparum multiple compartments. Conclusion The findings here reported reveal new features regarding ... |
format |
Article in Journal/Newspaper |
author |
Sarah El Chamy Maluf Marcelo Yudi Icimoto Pollyana Maria Saud Melo Alexandre Budu Rita Coimbra Marcos Leoni Gazarini Adriana Karaoglanovic Carmona |
author_facet |
Sarah El Chamy Maluf Marcelo Yudi Icimoto Pollyana Maria Saud Melo Alexandre Budu Rita Coimbra Marcos Leoni Gazarini Adriana Karaoglanovic Carmona |
author_sort |
Sarah El Chamy Maluf |
title |
Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes |
title_short |
Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes |
title_full |
Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes |
title_fullStr |
Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes |
title_full_unstemmed |
Human plasma plasminogen internalization route in Plasmodium falciparum-infected erythrocytes |
title_sort |
human plasma plasminogen internalization route in plasmodium falciparum-infected erythrocytes |
publisher |
BMC |
publishDate |
2020 |
url |
https://doi.org/10.1186/s12936-020-03377-4 https://doaj.org/article/87efd33c13dc411baf4741971195b93b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 19, Iss 1, Pp 1-10 (2020) |
op_relation |
http://link.springer.com/article/10.1186/s12936-020-03377-4 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03377-4 1475-2875 https://doaj.org/article/87efd33c13dc411baf4741971195b93b |
op_doi |
https://doi.org/10.1186/s12936-020-03377-4 |
container_title |
Malaria Journal |
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19 |
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1 |
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1766344329869131776 |