Osteopontin Is Upregulated in Human and Murine Acute Schistosomiasis Mansoni.
Symptomatic acute schistosomiasis mansoni is a systemic hypersensitivity reaction against the migrating schistosomula and mature eggs after a primary infection. The mechanisms involved in the pathogenesis of acute schistosomiasis are not fully elucidated. Osteopontin has been implicated in granuloma...
Published in: | PLOS Neglected Tropical Diseases |
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2016
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ftdoajarticles:oai:doaj.org/article:87e6146f05e549bbb4c479640be480d8 2023-05-15T15:15:37+02:00 Osteopontin Is Upregulated in Human and Murine Acute Schistosomiasis Mansoni. Thiago Almeida Pereira Wing-Kin Syn Frederico Figueiredo Amâncio Pedro Henrique Diniz Cunha Julia Fonseca Morais Caporali Guilherme Vaz de Melo Trindade Elisângela Trindade Santos Márcia Maria Souza Zilton Araújo Andrade Rafal P Witek William Evan Secor Fausto Edmundo Lima Pereira José Roberto Lambertucci Anna Mae Diehl 2016-10-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0005057 https://doaj.org/article/87e6146f05e549bbb4c479640be480d8 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC5068698?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005057 https://doaj.org/article/87e6146f05e549bbb4c479640be480d8 PLoS Neglected Tropical Diseases, Vol 10, Iss 10, p e0005057 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0005057 2022-12-31T03:21:51Z Symptomatic acute schistosomiasis mansoni is a systemic hypersensitivity reaction against the migrating schistosomula and mature eggs after a primary infection. The mechanisms involved in the pathogenesis of acute schistosomiasis are not fully elucidated. Osteopontin has been implicated in granulomatous reactions and in acute hepatic injury. Our aims were to evaluate if osteopontin plays a role in acute Schistosoma mansoni infection in both human and experimentally infected mice and if circulating OPN levels could be a novel biomarker of this infection.Serum/plasma osteopontin levels were measured by ELISA in patients with acute (n = 28), hepatointestinal (n = 26), hepatosplenic (n = 39) schistosomiasis and in uninfected controls (n = 21). Liver osteopontin was assessed by immunohistochemistry in needle biopsies of 5 patients. Sera and hepatic osteopontin were quantified in the murine model of schistosomiasis mansoni during acute (7 and 8 weeks post infection, n = 10) and chronic (30 weeks post infection, n = 8) phase. Circulating osteopontin levels are increased in patients with acute schistosomiasis (p = 0.0001). The highest levels of OPN were observed during the peak of clinical symptoms (7-11 weeks post infection), returning to baseline level once the granulomas were modulated (>12 weeks post infection). The plasma levels in acute schistosomiasis were even higher than in hepatosplenic patients. The murine model mirrored the human disease. Macrophages were the major source of OPN in human and murine acute schistosomiasis, while the ductular reaction maintains OPN production in hepatosplenic disease. Soluble egg antigens from S. mansoni induced OPN expression in primary human kupffer cells.S. mansoni egg antigens induce the production of OPN by macrophages in the necrotic-exudative granulomas characteristic of acute schistosomiasis mansoni. Circulating OPN levels are upregulated in human and murine acute schistosomiasis and could be a non-invasive biomarker of this form of disease. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 10 e0005057 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Thiago Almeida Pereira Wing-Kin Syn Frederico Figueiredo Amâncio Pedro Henrique Diniz Cunha Julia Fonseca Morais Caporali Guilherme Vaz de Melo Trindade Elisângela Trindade Santos Márcia Maria Souza Zilton Araújo Andrade Rafal P Witek William Evan Secor Fausto Edmundo Lima Pereira José Roberto Lambertucci Anna Mae Diehl Osteopontin Is Upregulated in Human and Murine Acute Schistosomiasis Mansoni. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Symptomatic acute schistosomiasis mansoni is a systemic hypersensitivity reaction against the migrating schistosomula and mature eggs after a primary infection. The mechanisms involved in the pathogenesis of acute schistosomiasis are not fully elucidated. Osteopontin has been implicated in granulomatous reactions and in acute hepatic injury. Our aims were to evaluate if osteopontin plays a role in acute Schistosoma mansoni infection in both human and experimentally infected mice and if circulating OPN levels could be a novel biomarker of this infection.Serum/plasma osteopontin levels were measured by ELISA in patients with acute (n = 28), hepatointestinal (n = 26), hepatosplenic (n = 39) schistosomiasis and in uninfected controls (n = 21). Liver osteopontin was assessed by immunohistochemistry in needle biopsies of 5 patients. Sera and hepatic osteopontin were quantified in the murine model of schistosomiasis mansoni during acute (7 and 8 weeks post infection, n = 10) and chronic (30 weeks post infection, n = 8) phase. Circulating osteopontin levels are increased in patients with acute schistosomiasis (p = 0.0001). The highest levels of OPN were observed during the peak of clinical symptoms (7-11 weeks post infection), returning to baseline level once the granulomas were modulated (>12 weeks post infection). The plasma levels in acute schistosomiasis were even higher than in hepatosplenic patients. The murine model mirrored the human disease. Macrophages were the major source of OPN in human and murine acute schistosomiasis, while the ductular reaction maintains OPN production in hepatosplenic disease. Soluble egg antigens from S. mansoni induced OPN expression in primary human kupffer cells.S. mansoni egg antigens induce the production of OPN by macrophages in the necrotic-exudative granulomas characteristic of acute schistosomiasis mansoni. Circulating OPN levels are upregulated in human and murine acute schistosomiasis and could be a non-invasive biomarker of this form of disease. |
format |
Article in Journal/Newspaper |
author |
Thiago Almeida Pereira Wing-Kin Syn Frederico Figueiredo Amâncio Pedro Henrique Diniz Cunha Julia Fonseca Morais Caporali Guilherme Vaz de Melo Trindade Elisângela Trindade Santos Márcia Maria Souza Zilton Araújo Andrade Rafal P Witek William Evan Secor Fausto Edmundo Lima Pereira José Roberto Lambertucci Anna Mae Diehl |
author_facet |
Thiago Almeida Pereira Wing-Kin Syn Frederico Figueiredo Amâncio Pedro Henrique Diniz Cunha Julia Fonseca Morais Caporali Guilherme Vaz de Melo Trindade Elisângela Trindade Santos Márcia Maria Souza Zilton Araújo Andrade Rafal P Witek William Evan Secor Fausto Edmundo Lima Pereira José Roberto Lambertucci Anna Mae Diehl |
author_sort |
Thiago Almeida Pereira |
title |
Osteopontin Is Upregulated in Human and Murine Acute Schistosomiasis Mansoni. |
title_short |
Osteopontin Is Upregulated in Human and Murine Acute Schistosomiasis Mansoni. |
title_full |
Osteopontin Is Upregulated in Human and Murine Acute Schistosomiasis Mansoni. |
title_fullStr |
Osteopontin Is Upregulated in Human and Murine Acute Schistosomiasis Mansoni. |
title_full_unstemmed |
Osteopontin Is Upregulated in Human and Murine Acute Schistosomiasis Mansoni. |
title_sort |
osteopontin is upregulated in human and murine acute schistosomiasis mansoni. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0005057 https://doaj.org/article/87e6146f05e549bbb4c479640be480d8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 10, p e0005057 (2016) |
op_relation |
http://europepmc.org/articles/PMC5068698?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0005057 https://doaj.org/article/87e6146f05e549bbb4c479640be480d8 |
op_doi |
https://doi.org/10.1371/journal.pntd.0005057 |
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PLOS Neglected Tropical Diseases |
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10 |
container_issue |
10 |
container_start_page |
e0005057 |
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