Growth Curve, Morphological and Molecular Characterization of Two Strains of Trypanosoma cruzi (Kinetoplastida, Trypanosomatidae) isolated from Triatoma sherlocki (Hemiptera, Reduviidae, Triatominae)

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Gabriela Kinue Watase Kunii, Rossana Falcone, Leandro da Costa Clementino, João Aristeu da Rosa, Juliana Damieli Nascimento, Tiago Belintani, Jader de Oliveira, Aline Rimoldi Ribeiro
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2022
• Subjects: Chagas disease; Trypanosoma cruzi; Epidemiology; Vector biology; Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doi.org/10.1590/0037-8682-0521-2021; https://doaj.org/article/87b48a8d173f4d6590e0b5b436004024

ABSTRACT Background: Trypanosoma cruzi presents great variability in morphology, virulence, pathogenicity, avoidance of the host immune system, and antigenic constitution, associated with different clinical manifestations of the disease. Methods: Two strains of T. cruzi were cultivated in liver infusion tryptose to determine growth kinetics, morphometry and molecular characterization using restriction fragment length polymorphism polymerase chain reaction. Results: The biological parameters showed sharp growth by the 7th day. Morphologically, both strains showed short and thin forms and were classified as Group I. Conclusion: Group TcI presents cardiac manifestations and T. sherlocki is adapting to the home environment, requiring attention to future problems.