Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes
Abstract Background Intraerythrocytic malaria parasites actively import obligate nutrients from serum and export proteins and lipids to erythrocyte cytoplasm and membrane. The import of macromolecules in the malaria parasite has been the subject of many debates. To understand the import of macromole...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2003
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-2-11 https://doaj.org/article/87b109bca0d0487aa888684db409b600 |
id |
ftdoajarticles:oai:doaj.org/article:87b109bca0d0487aa888684db409b600 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:87b109bca0d0487aa888684db409b600 2023-05-15T15:11:52+02:00 Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes Malhotra Pawan El Tahir Ahmed Chauhan Virander S 2003-05-01T00:00:00Z https://doi.org/10.1186/1475-2875-2-11 https://doaj.org/article/87b109bca0d0487aa888684db409b600 EN eng BMC http://www.malariajournal.com/content/2/1/11 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-11 1475-2875 https://doaj.org/article/87b109bca0d0487aa888684db409b600 Malaria Journal, Vol 2, Iss 1, p 11 (2003) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2003 ftdoajarticles https://doi.org/10.1186/1475-2875-2-11 2022-12-30T21:50:57Z Abstract Background Intraerythrocytic malaria parasites actively import obligate nutrients from serum and export proteins and lipids to erythrocyte cytoplasm and membrane. The import of macromolecules in the malaria parasite has been the subject of many debates. To understand the import of macromolecules by the parasite, we studied the uptake of proteins by Plasmodium falciparum infected human erythrocyte. Methods Proteins were biotin labelled individually, purified on a gel filtration column and added to uninfected and infected asynchronized culture. The uptake of these proteins by malaria parasites was determined by western blot analysis of parasite pellet and their different fractions using streptavidin-horseradish conjugate. To further confirm this import, we studied the uptake of 125 I-labelled proteins by western blot analysis as well as used direct immunofluorescence method. Results Here we show that biotin labelled and radio-iodinated polypeptides of molecular sizes in the range of 45 to 206 kDa, when added in the culture medium, get direct access to the parasite membrane through a membrane network by by-passing the erythrocyte cytosol. The import of these polypeptides is ATP-dependent as sodium azide treatment blocks this uptake. We also show that malaria parasites have the ability to take up and degrade biotin labelled human serum albumin, which has been shown to be essential for the parasite growth. Conclusions These results can be used, as a basis to explore the role of human serum albumin in the intraerythrocytic development of parasites, and this in turn can be an important adjunct to the development of novel antimalarial drugs. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 2 1 11 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Malhotra Pawan El Tahir Ahmed Chauhan Virander S Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Intraerythrocytic malaria parasites actively import obligate nutrients from serum and export proteins and lipids to erythrocyte cytoplasm and membrane. The import of macromolecules in the malaria parasite has been the subject of many debates. To understand the import of macromolecules by the parasite, we studied the uptake of proteins by Plasmodium falciparum infected human erythrocyte. Methods Proteins were biotin labelled individually, purified on a gel filtration column and added to uninfected and infected asynchronized culture. The uptake of these proteins by malaria parasites was determined by western blot analysis of parasite pellet and their different fractions using streptavidin-horseradish conjugate. To further confirm this import, we studied the uptake of 125 I-labelled proteins by western blot analysis as well as used direct immunofluorescence method. Results Here we show that biotin labelled and radio-iodinated polypeptides of molecular sizes in the range of 45 to 206 kDa, when added in the culture medium, get direct access to the parasite membrane through a membrane network by by-passing the erythrocyte cytosol. The import of these polypeptides is ATP-dependent as sodium azide treatment blocks this uptake. We also show that malaria parasites have the ability to take up and degrade biotin labelled human serum albumin, which has been shown to be essential for the parasite growth. Conclusions These results can be used, as a basis to explore the role of human serum albumin in the intraerythrocytic development of parasites, and this in turn can be an important adjunct to the development of novel antimalarial drugs. |
format |
Article in Journal/Newspaper |
author |
Malhotra Pawan El Tahir Ahmed Chauhan Virander S |
author_facet |
Malhotra Pawan El Tahir Ahmed Chauhan Virander S |
author_sort |
Malhotra Pawan |
title |
Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes |
title_short |
Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes |
title_full |
Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes |
title_fullStr |
Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes |
title_full_unstemmed |
Uptake of proteins and degradation of human serum albumin by Plasmodium falciparum – infected human erythrocytes |
title_sort |
uptake of proteins and degradation of human serum albumin by plasmodium falciparum – infected human erythrocytes |
publisher |
BMC |
publishDate |
2003 |
url |
https://doi.org/10.1186/1475-2875-2-11 https://doaj.org/article/87b109bca0d0487aa888684db409b600 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 2, Iss 1, p 11 (2003) |
op_relation |
http://www.malariajournal.com/content/2/1/11 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-11 1475-2875 https://doaj.org/article/87b109bca0d0487aa888684db409b600 |
op_doi |
https://doi.org/10.1186/1475-2875-2-11 |
container_title |
Malaria Journal |
container_volume |
2 |
container_issue |
1 |
container_start_page |
11 |
_version_ |
1766342659592421376 |