Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production
Objective: To investigate the effects of Agave sisalana (A. sisalana) extract on Aedes aegypti (Ae. aegypti) primary cell culture. Methods: Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract (0.18–6.00 mg/mL) for 24 h. Then, the cells were labeled with propidi...
Published in: | Asian Pacific Journal of Tropical Biomedicine |
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Wolters Kluwer Medknow Publications
2016
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ftdoajarticles:oai:doaj.org/article:87a36c6fbe5f4dfbbe86bd7dfee2e66d 2023-05-15T15:05:27+02:00 Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production Louise Helena Guimarães de Oliveira Patricia Alexandria Paiva Silva de Sousa Fabrine Felipe Hilario Gabriel Joventino Nascimento João Paulo Saraiva Morais Everaldo Paulo de Medeiros Manoel Francisco de Sousa Fabiola da Cruz Nunes 2016-05-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2015.12.018 https://doaj.org/article/87a36c6fbe5f4dfbbe86bd7dfee2e66d EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169116301927 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.12.018 https://doaj.org/article/87a36c6fbe5f4dfbbe86bd7dfee2e66d Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 5, Pp 396-399 (2016) Sisal Cell culture Mosquito Insecticide Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2016 ftdoajarticles https://doi.org/10.1016/j.apjtb.2015.12.018 2022-12-30T22:29:33Z Objective: To investigate the effects of Agave sisalana (A. sisalana) extract on Aedes aegypti (Ae. aegypti) primary cell culture. Methods: Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract (0.18–6.00 mg/mL) for 24 h. Then, the cells were labeled with propidium iodide and subjected to fluorescence microscopy to verify cell viability. In addition, nitric oxide production was measured. Results: Results showed that cells exposed to 6 mg/mL of the crude extract presented a greater percentage of death when compared to control (73.8% ± 9.6% vs. 34.6% ± 9.6%). Furthermore, there was an increase in the nitric oxide production in cells exposed to 6 mg/mL of A. sisalana crude extract [(0.81 ± 0.08) μmol/L] compared to control group [(0.41 ± 0.18) μmol/L]. Conclusions: The results show that A. sisalana is cytotoxic to Ae. aegypti and may be used as raw material for new eco-friendly and inexpensive insecticides, since sisal industry discards the liquid waste for the extraction of plant fiber. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 6 5 396 399 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Sisal Cell culture Mosquito Insecticide Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Sisal Cell culture Mosquito Insecticide Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Louise Helena Guimarães de Oliveira Patricia Alexandria Paiva Silva de Sousa Fabrine Felipe Hilario Gabriel Joventino Nascimento João Paulo Saraiva Morais Everaldo Paulo de Medeiros Manoel Francisco de Sousa Fabiola da Cruz Nunes Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production |
topic_facet |
Sisal Cell culture Mosquito Insecticide Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To investigate the effects of Agave sisalana (A. sisalana) extract on Aedes aegypti (Ae. aegypti) primary cell culture. Methods: Cells of Ae. aegypti were exposed to different concentrations of A. sisalana crude extract (0.18–6.00 mg/mL) for 24 h. Then, the cells were labeled with propidium iodide and subjected to fluorescence microscopy to verify cell viability. In addition, nitric oxide production was measured. Results: Results showed that cells exposed to 6 mg/mL of the crude extract presented a greater percentage of death when compared to control (73.8% ± 9.6% vs. 34.6% ± 9.6%). Furthermore, there was an increase in the nitric oxide production in cells exposed to 6 mg/mL of A. sisalana crude extract [(0.81 ± 0.08) μmol/L] compared to control group [(0.41 ± 0.18) μmol/L]. Conclusions: The results show that A. sisalana is cytotoxic to Ae. aegypti and may be used as raw material for new eco-friendly and inexpensive insecticides, since sisal industry discards the liquid waste for the extraction of plant fiber. |
format |
Article in Journal/Newspaper |
author |
Louise Helena Guimarães de Oliveira Patricia Alexandria Paiva Silva de Sousa Fabrine Felipe Hilario Gabriel Joventino Nascimento João Paulo Saraiva Morais Everaldo Paulo de Medeiros Manoel Francisco de Sousa Fabiola da Cruz Nunes |
author_facet |
Louise Helena Guimarães de Oliveira Patricia Alexandria Paiva Silva de Sousa Fabrine Felipe Hilario Gabriel Joventino Nascimento João Paulo Saraiva Morais Everaldo Paulo de Medeiros Manoel Francisco de Sousa Fabiola da Cruz Nunes |
author_sort |
Louise Helena Guimarães de Oliveira |
title |
Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production |
title_short |
Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production |
title_full |
Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production |
title_fullStr |
Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production |
title_full_unstemmed |
Agave sisalana extract induces cell death in Aedes aegypti hemocytes increasing nitric oxide production |
title_sort |
agave sisalana extract induces cell death in aedes aegypti hemocytes increasing nitric oxide production |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2016 |
url |
https://doi.org/10.1016/j.apjtb.2015.12.018 https://doaj.org/article/87a36c6fbe5f4dfbbe86bd7dfee2e66d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 6, Iss 5, Pp 396-399 (2016) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169116301927 https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2015.12.018 https://doaj.org/article/87a36c6fbe5f4dfbbe86bd7dfee2e66d |
op_doi |
https://doi.org/10.1016/j.apjtb.2015.12.018 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
6 |
container_issue |
5 |
container_start_page |
396 |
op_container_end_page |
399 |
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1766337150460100608 |