Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes
Introduction: Broccoli (Brassica oleracea) is well known for its properties as an anticancer, antioxidant, and scavenger of free radicals. However, its benefits in enhancing spermatogenesis have not been well established. Objective: To study broccoli aqueous extract effects on sperm factors and the...
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ftdoajarticles:oai:doaj.org/article:8786f566a04c464ba4483d5ea714e1ac 2023-05-15T15:12:15+02:00 Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes Omid Jazayeri Setareh Farahmand Araghi Tahereh A. Aghajanzadeh Fereshteh Mir Moammadrezaei 2021-12-01T00:00:00Z https://doi.org/10.7705/biomedica.5962 https://doaj.org/article/8786f566a04c464ba4483d5ea714e1ac EN ES eng spa Instituto Nacional de Salud https://revistabiomedica.org/index.php/biomedica/article/view/5962 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5962 https://doaj.org/article/8786f566a04c464ba4483d5ea714e1ac Biomédica: revista del Instituto Nacional de Salud, Vol 41, Iss 4, Pp 706-720 (2021) brassica spermatogenesis gene expression mice cadmium Medicine R Arctic medicine. Tropical medicine RC955-962 article 2021 ftdoajarticles https://doi.org/10.7705/biomedica.5962 2022-12-30T23:59:51Z Introduction: Broccoli (Brassica oleracea) is well known for its properties as an anticancer, antioxidant, and scavenger of free radicals. However, its benefits in enhancing spermatogenesis have not been well established. Objective: To study broccoli aqueous extract effects on sperm factors and the expression of genes Catsper1, Catsper2, Arl4a, Sox5, and Sox9 in sperm factors in mice. Material and methods: Male mice were divided randomly into six groups: (1) Control; (2) cadmium (3 mg/kg of mouse body weight); (3) orally treated with 200 μl broccoli aqueous extract (1 g ml-1); (4) orally treated with 400 μl of broccoli aqueous extract; (5) orally treated with 200 broccoli aqueous extract plus cadmium, and (6) orally treated with 400 μl of broccoli aqueous extract plus cadmium. We analyzed the sperms factors and Catsper1, Catsper2, Arl4a, Sox5, and Sox9 gene expression. Results: An obvious improvement in sperm count and a slight enhancement in sperm motility were observed in mice treated with broccoli extract alone or with cadmium. Sperm viability was reduced by broccoli extract except for the 200 μl dose with cadmium, which significantly increased it. Interestingly, Arl4a gene expression increased in the 400 μl broccoli-treated group. Likewise, the Arl4a mRNA level in mice treated with cadmium and 200 μl of broccoli extract was higher than in the cadmium-treated mice. Furthermore, broccoli extract enhanced the mRNA level of Catsper2 and Sox5 genes in mice treated with 200 μl and 400 μl broccoli extract plus cadmium compared with the group treated solely with cadmium. Conclusion: The higher sperm count in broccoli-treated mice opens the way for the development of pharmaceutical products for infertile men. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 41 4 706 720 |
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brassica spermatogenesis gene expression mice cadmium Medicine R Arctic medicine. Tropical medicine RC955-962 |
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brassica spermatogenesis gene expression mice cadmium Medicine R Arctic medicine. Tropical medicine RC955-962 Omid Jazayeri Setareh Farahmand Araghi Tahereh A. Aghajanzadeh Fereshteh Mir Moammadrezaei Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes |
topic_facet |
brassica spermatogenesis gene expression mice cadmium Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Broccoli (Brassica oleracea) is well known for its properties as an anticancer, antioxidant, and scavenger of free radicals. However, its benefits in enhancing spermatogenesis have not been well established. Objective: To study broccoli aqueous extract effects on sperm factors and the expression of genes Catsper1, Catsper2, Arl4a, Sox5, and Sox9 in sperm factors in mice. Material and methods: Male mice were divided randomly into six groups: (1) Control; (2) cadmium (3 mg/kg of mouse body weight); (3) orally treated with 200 μl broccoli aqueous extract (1 g ml-1); (4) orally treated with 400 μl of broccoli aqueous extract; (5) orally treated with 200 broccoli aqueous extract plus cadmium, and (6) orally treated with 400 μl of broccoli aqueous extract plus cadmium. We analyzed the sperms factors and Catsper1, Catsper2, Arl4a, Sox5, and Sox9 gene expression. Results: An obvious improvement in sperm count and a slight enhancement in sperm motility were observed in mice treated with broccoli extract alone or with cadmium. Sperm viability was reduced by broccoli extract except for the 200 μl dose with cadmium, which significantly increased it. Interestingly, Arl4a gene expression increased in the 400 μl broccoli-treated group. Likewise, the Arl4a mRNA level in mice treated with cadmium and 200 μl of broccoli extract was higher than in the cadmium-treated mice. Furthermore, broccoli extract enhanced the mRNA level of Catsper2 and Sox5 genes in mice treated with 200 μl and 400 μl broccoli extract plus cadmium compared with the group treated solely with cadmium. Conclusion: The higher sperm count in broccoli-treated mice opens the way for the development of pharmaceutical products for infertile men. |
format |
Article in Journal/Newspaper |
author |
Omid Jazayeri Setareh Farahmand Araghi Tahereh A. Aghajanzadeh Fereshteh Mir Moammadrezaei |
author_facet |
Omid Jazayeri Setareh Farahmand Araghi Tahereh A. Aghajanzadeh Fereshteh Mir Moammadrezaei |
author_sort |
Omid Jazayeri |
title |
Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes |
title_short |
Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes |
title_full |
Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes |
title_fullStr |
Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes |
title_full_unstemmed |
Up-regulation of Arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes |
title_sort |
up-regulation of arl4a gene expression by broccoli aqueous extract is associated with improved spermatogenesis in mouse testes |
publisher |
Instituto Nacional de Salud |
publishDate |
2021 |
url |
https://doi.org/10.7705/biomedica.5962 https://doaj.org/article/8786f566a04c464ba4483d5ea714e1ac |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 41, Iss 4, Pp 706-720 (2021) |
op_relation |
https://revistabiomedica.org/index.php/biomedica/article/view/5962 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.5962 https://doaj.org/article/8786f566a04c464ba4483d5ea714e1ac |
op_doi |
https://doi.org/10.7705/biomedica.5962 |
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Biomédica |
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41 |
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706 |
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720 |
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