Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos.
Mycobacterium leprae (M. leprae) is a human pathogen and the causative agent for leprosy, a chronic disease characterized by lesions of the skin and peripheral nerve damage. Zoonotic transmission of M. leprae to humans by nine-banded armadillos (Dasypus novemcinctus) has been shown to occur in the s...
Published in: | PLOS Neglected Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:870e3f1f0a95472baa398d3a1439a223 2023-05-15T15:17:43+02:00 Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. Moises B da Silva Juliana M Portela Wei Li Mary Jackson Mercedes Gonzalez-Juarrero Andrea Sánchez Hidalgo John T Belisle Raquel C Bouth Angélica R Gobbo Josafá G Barreto Antonio H H Minervino Stewart T Cole Charlotte Avanzi Philippe Busso Marco A C Frade Annemieke Geluk Claudio G Salgado John S Spencer 2018-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0006532 https://doaj.org/article/870e3f1f0a95472baa398d3a1439a223 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6023134?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006532 https://doaj.org/article/870e3f1f0a95472baa398d3a1439a223 PLoS Neglected Tropical Diseases, Vol 12, Iss 6, p e0006532 (2018) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2018 ftdoajarticles https://doi.org/10.1371/journal.pntd.0006532 2022-12-31T01:54:46Z Mycobacterium leprae (M. leprae) is a human pathogen and the causative agent for leprosy, a chronic disease characterized by lesions of the skin and peripheral nerve damage. Zoonotic transmission of M. leprae to humans by nine-banded armadillos (Dasypus novemcinctus) has been shown to occur in the southern United States, mainly in Texas, Louisiana, and Florida. Nine-banded armadillos are also common in South America, and residents living in some areas in Brazil hunt and kill armadillos as a dietary source of protein. This study examines the extent of M. leprae infection in wild armadillos and whether these New World mammals may be a natural reservoir for leprosy transmission in Brazil, similar to the situation in the southern states of the U.S. The presence of the M. leprae-specific repetitive sequence RLEP was detected by PCR amplification in purified DNA extracted from armadillo spleen and liver tissue samples. A positive RLEP signal was confirmed in 62% of the armadillos (10/16), indicating high rates of infection with M. leprae. Immunohistochemistry of sections of infected armadillo spleens revealed mycobacterial DNA and cell wall constituents in situ detected by SYBR Gold and auramine/rhodamine staining techniques, respectively. The M. leprae-specific antigen, phenolic glycolipid I (PGL-I) was detected in spleen sections using a rabbit polyclonal antibody specific for PGL-I. Anti-PGL-I titers were assessed by ELISA in sera from 146 inhabitants of Belterra, a hyperendemic city located in western Pará state in Brazil. A positive anti-PGL-I titer is a known biomarker for M. leprae infection in both humans and armadillos. Individuals who consumed armadillo meat most frequently (more than once per month) showed a significantly higher anti-PGL-I titer than those who did not eat or ate less frequently than once per month. Armadillos infected with M. leprae represent a potential environmental reservoir. Consequently, people who hunt, kill, or process or eat armadillo meat are at a higher risk for infection with M. ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 12 6 e0006532 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Moises B da Silva Juliana M Portela Wei Li Mary Jackson Mercedes Gonzalez-Juarrero Andrea Sánchez Hidalgo John T Belisle Raquel C Bouth Angélica R Gobbo Josafá G Barreto Antonio H H Minervino Stewart T Cole Charlotte Avanzi Philippe Busso Marco A C Frade Annemieke Geluk Claudio G Salgado John S Spencer Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Mycobacterium leprae (M. leprae) is a human pathogen and the causative agent for leprosy, a chronic disease characterized by lesions of the skin and peripheral nerve damage. Zoonotic transmission of M. leprae to humans by nine-banded armadillos (Dasypus novemcinctus) has been shown to occur in the southern United States, mainly in Texas, Louisiana, and Florida. Nine-banded armadillos are also common in South America, and residents living in some areas in Brazil hunt and kill armadillos as a dietary source of protein. This study examines the extent of M. leprae infection in wild armadillos and whether these New World mammals may be a natural reservoir for leprosy transmission in Brazil, similar to the situation in the southern states of the U.S. The presence of the M. leprae-specific repetitive sequence RLEP was detected by PCR amplification in purified DNA extracted from armadillo spleen and liver tissue samples. A positive RLEP signal was confirmed in 62% of the armadillos (10/16), indicating high rates of infection with M. leprae. Immunohistochemistry of sections of infected armadillo spleens revealed mycobacterial DNA and cell wall constituents in situ detected by SYBR Gold and auramine/rhodamine staining techniques, respectively. The M. leprae-specific antigen, phenolic glycolipid I (PGL-I) was detected in spleen sections using a rabbit polyclonal antibody specific for PGL-I. Anti-PGL-I titers were assessed by ELISA in sera from 146 inhabitants of Belterra, a hyperendemic city located in western Pará state in Brazil. A positive anti-PGL-I titer is a known biomarker for M. leprae infection in both humans and armadillos. Individuals who consumed armadillo meat most frequently (more than once per month) showed a significantly higher anti-PGL-I titer than those who did not eat or ate less frequently than once per month. Armadillos infected with M. leprae represent a potential environmental reservoir. Consequently, people who hunt, kill, or process or eat armadillo meat are at a higher risk for infection with M. ... |
format |
Article in Journal/Newspaper |
author |
Moises B da Silva Juliana M Portela Wei Li Mary Jackson Mercedes Gonzalez-Juarrero Andrea Sánchez Hidalgo John T Belisle Raquel C Bouth Angélica R Gobbo Josafá G Barreto Antonio H H Minervino Stewart T Cole Charlotte Avanzi Philippe Busso Marco A C Frade Annemieke Geluk Claudio G Salgado John S Spencer |
author_facet |
Moises B da Silva Juliana M Portela Wei Li Mary Jackson Mercedes Gonzalez-Juarrero Andrea Sánchez Hidalgo John T Belisle Raquel C Bouth Angélica R Gobbo Josafá G Barreto Antonio H H Minervino Stewart T Cole Charlotte Avanzi Philippe Busso Marco A C Frade Annemieke Geluk Claudio G Salgado John S Spencer |
author_sort |
Moises B da Silva |
title |
Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. |
title_short |
Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. |
title_full |
Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. |
title_fullStr |
Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. |
title_full_unstemmed |
Evidence of zoonotic leprosy in Pará, Brazilian Amazon, and risks associated with human contact or consumption of armadillos. |
title_sort |
evidence of zoonotic leprosy in pará, brazilian amazon, and risks associated with human contact or consumption of armadillos. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2018 |
url |
https://doi.org/10.1371/journal.pntd.0006532 https://doaj.org/article/870e3f1f0a95472baa398d3a1439a223 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 12, Iss 6, p e0006532 (2018) |
op_relation |
http://europepmc.org/articles/PMC6023134?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0006532 https://doaj.org/article/870e3f1f0a95472baa398d3a1439a223 |
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https://doi.org/10.1371/journal.pntd.0006532 |
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PLOS Neglected Tropical Diseases |
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