Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue periférico, de pacientes imunocompetentes e imunodeprimidos por meio de três amplificações duplas

Bacteria of the genus Bartonella are emerging pathogens detected in lymph node biopsies and aspirates probably caused by increased concentration of bacteria. Twenty-three samples of 18 patients with clinical, laboratory and/or epidemiological data suggesting bartonellosis were subjected to three nes...

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Main Authors: Karina Hatamoto Kawasato, Léa Campos de Oliveira, Paulo Eduardo Neves Ferreira Velho, Lidia Yamamoto, Gilda Maria Barbaro Del Negro, Thelma Suely Okay
Format: Article in Journal/Newspaper
Language:English
Published: Universidade de São Paulo (USP) 2013
Subjects:
PCR
Online Access:https://doaj.org/article/8708793f93ba4ee7b7cd4477c9eff567
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spelling ftdoajarticles:oai:doaj.org/article:8708793f93ba4ee7b7cd4477c9eff567 2024-09-09T19:28:40+00:00 Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue periférico, de pacientes imunocompetentes e imunodeprimidos por meio de três amplificações duplas Karina Hatamoto Kawasato Léa Campos de Oliveira Paulo Eduardo Neves Ferreira Velho Lidia Yamamoto Gilda Maria Barbaro Del Negro Thelma Suely Okay 2013-02-01T00:00:00Z https://doaj.org/article/8708793f93ba4ee7b7cd4477c9eff567 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000100001 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 0036-4665 1678-9946 https://doaj.org/article/8708793f93ba4ee7b7cd4477c9eff567 Revista do Instituto de Medicina Tropical de São Paulo, Vol 55, Iss 1, Pp 1-6 (2013) Bartonellosis Bartonella spp Bartonella henselae Cat Scratch Disease PCR Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2013 ftdoajarticles 2024-08-05T17:49:31Z Bacteria of the genus Bartonella are emerging pathogens detected in lymph node biopsies and aspirates probably caused by increased concentration of bacteria. Twenty-three samples of 18 patients with clinical, laboratory and/or epidemiological data suggesting bartonellosis were subjected to three nested amplifications targeting a fragment of the 60-kDa heat shock protein (HSP), the internal transcribed spacer 16S-23S rRNA (ITS) and the cell division (FtsZ) of Bartonella henselae, in order to improve detection in clinical samples. In the first amplification 01, 04 and 05 samples, were positive by HSP (4.3%), FtsZ (17.4%) and ITS (21.7%), respectively. After the second round six positive samples were identified by nested-HSP (26%), eight by nested-ITS (34.8%) and 18 by nested-FtsZ (78.2%), corresponding to 10 peripheral blood samples, five lymph node biopsies, two skin biopsies and one lymph node aspirate. The nested-FtsZ was more sensitive than nested-HSP and nested-ITS (p < 0.0001), enabling the detection of Bartonella henselae DNA in 15 of 18 patients (83.3%). In this study, three nested-PCR that should be specific for Bartonella henselae amplification were developed, but only the nested-FtsZ did not amplify DNA from Bartonella quintana. We conclude that nested amplifications increased detection of B. henselae DNA, and that the nested-FtsZ was the most sensitive and the only specific to B. henselae in different biological samples. As all samples detected by nested-HSP and nested-ITS, were also by nested-FtsZ, we infer that in our series infections were caused by Bartonella henselae. The high number of positive blood samples draws attention to the use of this biological material in the investigation of bartonellosis, regardless of the immune status of patients. This fact is important in the case of critically ill patients and young children to avoid more invasive procedures such as lymph nodes biopsies and aspirates. Bactérias do gênero Bartonella constituem patógenos emergentes detectados em biópsias de ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
topic Bartonellosis
Bartonella spp
Bartonella henselae
Cat Scratch Disease
PCR
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
spellingShingle Bartonellosis
Bartonella spp
Bartonella henselae
Cat Scratch Disease
PCR
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
Karina Hatamoto Kawasato
Léa Campos de Oliveira
Paulo Eduardo Neves Ferreira Velho
Lidia Yamamoto
Gilda Maria Barbaro Del Negro
Thelma Suely Okay
Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue periférico, de pacientes imunocompetentes e imunodeprimidos por meio de três amplificações duplas
topic_facet Bartonellosis
Bartonella spp
Bartonella henselae
Cat Scratch Disease
PCR
Arctic medicine. Tropical medicine
RC955-962
Infectious and parasitic diseases
RC109-216
description Bacteria of the genus Bartonella are emerging pathogens detected in lymph node biopsies and aspirates probably caused by increased concentration of bacteria. Twenty-three samples of 18 patients with clinical, laboratory and/or epidemiological data suggesting bartonellosis were subjected to three nested amplifications targeting a fragment of the 60-kDa heat shock protein (HSP), the internal transcribed spacer 16S-23S rRNA (ITS) and the cell division (FtsZ) of Bartonella henselae, in order to improve detection in clinical samples. In the first amplification 01, 04 and 05 samples, were positive by HSP (4.3%), FtsZ (17.4%) and ITS (21.7%), respectively. After the second round six positive samples were identified by nested-HSP (26%), eight by nested-ITS (34.8%) and 18 by nested-FtsZ (78.2%), corresponding to 10 peripheral blood samples, five lymph node biopsies, two skin biopsies and one lymph node aspirate. The nested-FtsZ was more sensitive than nested-HSP and nested-ITS (p < 0.0001), enabling the detection of Bartonella henselae DNA in 15 of 18 patients (83.3%). In this study, three nested-PCR that should be specific for Bartonella henselae amplification were developed, but only the nested-FtsZ did not amplify DNA from Bartonella quintana. We conclude that nested amplifications increased detection of B. henselae DNA, and that the nested-FtsZ was the most sensitive and the only specific to B. henselae in different biological samples. As all samples detected by nested-HSP and nested-ITS, were also by nested-FtsZ, we infer that in our series infections were caused by Bartonella henselae. The high number of positive blood samples draws attention to the use of this biological material in the investigation of bartonellosis, regardless of the immune status of patients. This fact is important in the case of critically ill patients and young children to avoid more invasive procedures such as lymph nodes biopsies and aspirates. Bactérias do gênero Bartonella constituem patógenos emergentes detectados em biópsias de ...
format Article in Journal/Newspaper
author Karina Hatamoto Kawasato
Léa Campos de Oliveira
Paulo Eduardo Neves Ferreira Velho
Lidia Yamamoto
Gilda Maria Barbaro Del Negro
Thelma Suely Okay
author_facet Karina Hatamoto Kawasato
Léa Campos de Oliveira
Paulo Eduardo Neves Ferreira Velho
Lidia Yamamoto
Gilda Maria Barbaro Del Negro
Thelma Suely Okay
author_sort Karina Hatamoto Kawasato
title Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue periférico, de pacientes imunocompetentes e imunodeprimidos por meio de três amplificações duplas
title_short Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue periférico, de pacientes imunocompetentes e imunodeprimidos por meio de três amplificações duplas
title_full Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue periférico, de pacientes imunocompetentes e imunodeprimidos por meio de três amplificações duplas
title_fullStr Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue periférico, de pacientes imunocompetentes e imunodeprimidos por meio de três amplificações duplas
title_full_unstemmed Detection of Bartonella henselae DNA in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications Detecção de DNA de Bartonella henselae em amostras clínicas, incluindo sangue periférico, de pacientes imunocompetentes e imunodeprimidos por meio de três amplificações duplas
title_sort detection of bartonella henselae dna in clinical samples including peripheral blood of immune competent and immune compromised patients by three nested amplifications detecção de dna de bartonella henselae em amostras clínicas, incluindo sangue periférico, de pacientes imunocompetentes e imunodeprimidos por meio de três amplificações duplas
publisher Universidade de São Paulo (USP)
publishDate 2013
url https://doaj.org/article/8708793f93ba4ee7b7cd4477c9eff567
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Revista do Instituto de Medicina Tropical de São Paulo, Vol 55, Iss 1, Pp 1-6 (2013)
op_relation http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46652013000100001
https://doaj.org/toc/0036-4665
https://doaj.org/toc/1678-9946
0036-4665
1678-9946
https://doaj.org/article/8708793f93ba4ee7b7cd4477c9eff567
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