Aedes aegypti SGS1 is critical for Plasmodium gallinaceum infection of both the mosquito midgut and salivary glands
Abstract Background The invasion of the mosquito salivary glands by Plasmodium sporozoites is a critical step that defines the success of malaria transmission and a detailed understanding of the molecules responsible for salivary gland invasion could be leveraged towards control of vector-borne path...
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ftdoajarticles:oai:doaj.org/article:86f778f4fc9c4099a700856a80cd99ff 2023-05-15T15:11:43+02:00 Aedes aegypti SGS1 is critical for Plasmodium gallinaceum infection of both the mosquito midgut and salivary glands Bianca B. Kojin Ines Martin-Martin Helena R. C. Araújo Brian Bonilla Alvaro Molina-Cruz Eric Calvo Margareth L. Capurro Zach N. Adelman 2021-01-01T00:00:00Z https://doi.org/10.1186/s12936-020-03537-6 https://doaj.org/article/86f778f4fc9c4099a700856a80cd99ff EN eng BMC https://doi.org/10.1186/s12936-020-03537-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03537-6 1475-2875 https://doaj.org/article/86f778f4fc9c4099a700856a80cd99ff Malaria Journal, Vol 20, Iss 1, Pp 1-15 (2021) Aedes aegypti Plasmodium gallinaceum Malaria Salivary gland SGS1 Sporozoites Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-020-03537-6 2022-12-31T07:29:06Z Abstract Background The invasion of the mosquito salivary glands by Plasmodium sporozoites is a critical step that defines the success of malaria transmission and a detailed understanding of the molecules responsible for salivary gland invasion could be leveraged towards control of vector-borne pathogens. Antibodies directed against the mosquito salivary gland protein SGS1 have been shown to reduce Plasmodium gallinaceum sporozoite invasion of Aedes aegypti salivary glands, but the specific role of this protein in sporozoite invasion and in other stages of the Plasmodium life cycle remains unknown. Methods RNA interference and CRISPR/Cas9 were used to evaluate the role of A. aegypti SGS1 in the P. gallinaceum life cycle. Results Knockdown and knockout of SGS1 disrupted sporozoite invasion of the salivary gland. Interestingly, mosquitoes lacking SGS1 also displayed fewer oocysts. Proteomic analyses confirmed the abolishment of SGS1 in the salivary gland of SGS1 knockout mosquitoes and revealed that the C-terminus of the protein is absent in the salivary gland of control mosquitoes. In silico analyses indicated that SGS1 contains two potential internal cleavage sites and thus might generate three proteins. Conclusion SGS1 facilitates, but is not essential for, invasion of A. aegypti salivary glands by P. gallinaceum and has a dual role as a facilitator of parasite development in the mosquito midgut. SGS1 could, therefore, be part of a strategy to decrease malaria transmission by the mosquito vector, for example in a transgenic mosquito that blocks its interaction with the parasite. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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Directory of Open Access Journals: DOAJ Articles |
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topic |
Aedes aegypti Plasmodium gallinaceum Malaria Salivary gland SGS1 Sporozoites Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Aedes aegypti Plasmodium gallinaceum Malaria Salivary gland SGS1 Sporozoites Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Bianca B. Kojin Ines Martin-Martin Helena R. C. Araújo Brian Bonilla Alvaro Molina-Cruz Eric Calvo Margareth L. Capurro Zach N. Adelman Aedes aegypti SGS1 is critical for Plasmodium gallinaceum infection of both the mosquito midgut and salivary glands |
topic_facet |
Aedes aegypti Plasmodium gallinaceum Malaria Salivary gland SGS1 Sporozoites Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The invasion of the mosquito salivary glands by Plasmodium sporozoites is a critical step that defines the success of malaria transmission and a detailed understanding of the molecules responsible for salivary gland invasion could be leveraged towards control of vector-borne pathogens. Antibodies directed against the mosquito salivary gland protein SGS1 have been shown to reduce Plasmodium gallinaceum sporozoite invasion of Aedes aegypti salivary glands, but the specific role of this protein in sporozoite invasion and in other stages of the Plasmodium life cycle remains unknown. Methods RNA interference and CRISPR/Cas9 were used to evaluate the role of A. aegypti SGS1 in the P. gallinaceum life cycle. Results Knockdown and knockout of SGS1 disrupted sporozoite invasion of the salivary gland. Interestingly, mosquitoes lacking SGS1 also displayed fewer oocysts. Proteomic analyses confirmed the abolishment of SGS1 in the salivary gland of SGS1 knockout mosquitoes and revealed that the C-terminus of the protein is absent in the salivary gland of control mosquitoes. In silico analyses indicated that SGS1 contains two potential internal cleavage sites and thus might generate three proteins. Conclusion SGS1 facilitates, but is not essential for, invasion of A. aegypti salivary glands by P. gallinaceum and has a dual role as a facilitator of parasite development in the mosquito midgut. SGS1 could, therefore, be part of a strategy to decrease malaria transmission by the mosquito vector, for example in a transgenic mosquito that blocks its interaction with the parasite. |
format |
Article in Journal/Newspaper |
author |
Bianca B. Kojin Ines Martin-Martin Helena R. C. Araújo Brian Bonilla Alvaro Molina-Cruz Eric Calvo Margareth L. Capurro Zach N. Adelman |
author_facet |
Bianca B. Kojin Ines Martin-Martin Helena R. C. Araújo Brian Bonilla Alvaro Molina-Cruz Eric Calvo Margareth L. Capurro Zach N. Adelman |
author_sort |
Bianca B. Kojin |
title |
Aedes aegypti SGS1 is critical for Plasmodium gallinaceum infection of both the mosquito midgut and salivary glands |
title_short |
Aedes aegypti SGS1 is critical for Plasmodium gallinaceum infection of both the mosquito midgut and salivary glands |
title_full |
Aedes aegypti SGS1 is critical for Plasmodium gallinaceum infection of both the mosquito midgut and salivary glands |
title_fullStr |
Aedes aegypti SGS1 is critical for Plasmodium gallinaceum infection of both the mosquito midgut and salivary glands |
title_full_unstemmed |
Aedes aegypti SGS1 is critical for Plasmodium gallinaceum infection of both the mosquito midgut and salivary glands |
title_sort |
aedes aegypti sgs1 is critical for plasmodium gallinaceum infection of both the mosquito midgut and salivary glands |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-020-03537-6 https://doaj.org/article/86f778f4fc9c4099a700856a80cd99ff |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-15 (2021) |
op_relation |
https://doi.org/10.1186/s12936-020-03537-6 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-020-03537-6 1475-2875 https://doaj.org/article/86f778f4fc9c4099a700856a80cd99ff |
op_doi |
https://doi.org/10.1186/s12936-020-03537-6 |
container_title |
Malaria Journal |
container_volume |
20 |
container_issue |
1 |
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1766342529688535040 |