Molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China
Abstract Background The emergence and spread of multidrug resistance poses a significant risk to malaria control and eradication goals in the world. There has been no indigenous malaria cases reported in China since 2017, and China is approaching national malaria elimination. Therefore, anti-malaria...
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ftdoajarticles:oai:doaj.org/article:86f38cbff16e4254b8dce842c6f11ec0 2023-05-15T15:17:36+02:00 Molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China Fang Huang He Yan Jing-Bo Xue Yan-Wen Cui Shui-Sen Zhou Zhi-Gui Xia Rabindra Abeyasinghe Pascal Ringwald Xiao-Nong Zhou 2021-02-01T00:00:00Z https://doi.org/10.1186/s12936-021-03613-5 https://doaj.org/article/86f38cbff16e4254b8dce842c6f11ec0 EN eng BMC https://doi.org/10.1186/s12936-021-03613-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03613-5 1475-2875 https://doaj.org/article/86f38cbff16e4254b8dce842c6f11ec0 Malaria Journal, Vol 20, Iss 1, Pp 1-11 (2021) Plasmodium falciparum Molecular markers Drug resistance Africa China Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2021 ftdoajarticles https://doi.org/10.1186/s12936-021-03613-5 2022-12-31T06:54:43Z Abstract Background The emergence and spread of multidrug resistance poses a significant risk to malaria control and eradication goals in the world. There has been no indigenous malaria cases reported in China since 2017, and China is approaching national malaria elimination. Therefore, anti-malarial drug resistance surveillance and tracking the emergence and spread of imported drug-resistant malaria cases will be necessary in a post-elimination phase in China. Methods Dried blood spots were obtained from Plasmodium falciparum-infected cases returned from Africa to China between 2012 and 2015, prior to anti-malarial drug treatment. Whole DNA were extracted and known polymorphisms relating to drug resistance of pfcrt, pfmdr1 gene, and the propeller domain of pfk13 were evaluated by nested PCR and sequencing. The haplotypes and prevalence of these three genes were evaluated separately. Chi-squared test and Fisher's exact test were used to evaluate differences among the different sub-regions of Africa. A P value < 0.05 was used to evaluate differences with statistical significance. The maps were created using ArcGIS. Results A total of 731 P. falciparum isolates were sequenced at the pfcrt locus. The wild type CVMNK was the most prevalent haplotype with prevalence of 62.8% and 29.8% of the isolates showed the triple mutant haplotype CVIET. A total of 434 P. falciparum isolates were successfully sequenced and pfmdr1 allelic variants were observed in only codons 86, 184 and 1246. Twelve haplotypes were identified and the prevalence of the wild type pfmdr1 NYD was 44.1%. The single mutant pfmdr1 in codons 86 and 184 was predominant but the haplotype NYY with single mutation in codon 1246 was not observed. The double mutant haplotype YFD was common in Africa. About 1,357 isolates were successfully sequenced of pfk13-propeller domain, the wild type was found in 1,308 samples (96.4%) whereby 49 samples (3.6%) had mutation in pfk13. Of 49 samples with pfk13 mutations, 22 non-synonymous and 4 synonymous polymorphic ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 20 1 |
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English |
topic |
Plasmodium falciparum Molecular markers Drug resistance Africa China Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium falciparum Molecular markers Drug resistance Africa China Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Fang Huang He Yan Jing-Bo Xue Yan-Wen Cui Shui-Sen Zhou Zhi-Gui Xia Rabindra Abeyasinghe Pascal Ringwald Xiao-Nong Zhou Molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China |
topic_facet |
Plasmodium falciparum Molecular markers Drug resistance Africa China Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The emergence and spread of multidrug resistance poses a significant risk to malaria control and eradication goals in the world. There has been no indigenous malaria cases reported in China since 2017, and China is approaching national malaria elimination. Therefore, anti-malarial drug resistance surveillance and tracking the emergence and spread of imported drug-resistant malaria cases will be necessary in a post-elimination phase in China. Methods Dried blood spots were obtained from Plasmodium falciparum-infected cases returned from Africa to China between 2012 and 2015, prior to anti-malarial drug treatment. Whole DNA were extracted and known polymorphisms relating to drug resistance of pfcrt, pfmdr1 gene, and the propeller domain of pfk13 were evaluated by nested PCR and sequencing. The haplotypes and prevalence of these three genes were evaluated separately. Chi-squared test and Fisher's exact test were used to evaluate differences among the different sub-regions of Africa. A P value < 0.05 was used to evaluate differences with statistical significance. The maps were created using ArcGIS. Results A total of 731 P. falciparum isolates were sequenced at the pfcrt locus. The wild type CVMNK was the most prevalent haplotype with prevalence of 62.8% and 29.8% of the isolates showed the triple mutant haplotype CVIET. A total of 434 P. falciparum isolates were successfully sequenced and pfmdr1 allelic variants were observed in only codons 86, 184 and 1246. Twelve haplotypes were identified and the prevalence of the wild type pfmdr1 NYD was 44.1%. The single mutant pfmdr1 in codons 86 and 184 was predominant but the haplotype NYY with single mutation in codon 1246 was not observed. The double mutant haplotype YFD was common in Africa. About 1,357 isolates were successfully sequenced of pfk13-propeller domain, the wild type was found in 1,308 samples (96.4%) whereby 49 samples (3.6%) had mutation in pfk13. Of 49 samples with pfk13 mutations, 22 non-synonymous and 4 synonymous polymorphic ... |
format |
Article in Journal/Newspaper |
author |
Fang Huang He Yan Jing-Bo Xue Yan-Wen Cui Shui-Sen Zhou Zhi-Gui Xia Rabindra Abeyasinghe Pascal Ringwald Xiao-Nong Zhou |
author_facet |
Fang Huang He Yan Jing-Bo Xue Yan-Wen Cui Shui-Sen Zhou Zhi-Gui Xia Rabindra Abeyasinghe Pascal Ringwald Xiao-Nong Zhou |
author_sort |
Fang Huang |
title |
Molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China |
title_short |
Molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China |
title_full |
Molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China |
title_fullStr |
Molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China |
title_full_unstemmed |
Molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in Plasmodium falciparum isolates imported from Africa to China |
title_sort |
molecular surveillance of pfcrt, pfmdr1 and pfk13-propeller mutations in plasmodium falciparum isolates imported from africa to china |
publisher |
BMC |
publishDate |
2021 |
url |
https://doi.org/10.1186/s12936-021-03613-5 https://doaj.org/article/86f38cbff16e4254b8dce842c6f11ec0 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 20, Iss 1, Pp 1-11 (2021) |
op_relation |
https://doi.org/10.1186/s12936-021-03613-5 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-021-03613-5 1475-2875 https://doaj.org/article/86f38cbff16e4254b8dce842c6f11ec0 |
op_doi |
https://doi.org/10.1186/s12936-021-03613-5 |
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Malaria Journal |
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20 |
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1 |
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1766347846968147968 |