Detection of Leishmania (Viannia) DNA in leucocytes from the blood of patients with cutaneous leishmaniasis

• Published in: Revista da Sociedade Brasileira de Medicina Tropical
• Main Authors: Carolina Cella Conter, Herintha Coeto Neitzke-Abreu, Raissa Bocchi Pedroso, Maria Valdrinez Campana Lonardoni, Thaís Gomes Verzignassi Silveira, Sandra Mara Alessi Aristides
• Format: Article in Journal/Newspaper
• Language: English
• Published: Sociedade Brasileira de Medicina Tropical (SBMT) 2015
• Subjects: Polymerase chain reaction; Leishmania ( Viannia ); Leucocyte; Arctic medicine. Tropical medicine; RC955-962; Arctic
• Online Access: https://doi.org/10.1590/0037-8682-0052-2015; https://doaj.org/article/86edcbf7ef0643269daf7ac649bb4f0c

ABSTRACTINTRODUCTION:Cutaneous leishmaniasis (CL) is a serious and global public health issue, with the potential of developing a mucosal form, occurring as subclinical cases, and showing recurrence despite previous treatment.METHODS:Polymorphonuclear and mononuclear DNA obtained from 49 patients was subjected to polymerase chain reaction for detection of Leishmania (Viannia).RESULTS:DNA was detected in mononuclear cells from two patients with active primary lesions positive for CL, with infection periods of 3 and 6 months, respectively.CONCLUSIONS:The DNA of Leishmania (Viannia) indicates probable parasite dissemination possibly explaining subclinical case emergence, lesion recurrence, and mucosal lesion appearance.