Polymorphism in the HASPB repeat region of East African Leishmania donovani strains.
BACKGROUND/OBJECTIVES: Visceral leishmaniasis (VL) caused by Leishmania donovani is a major health problem in Ethiopia. Parasites in disparate regions are transmitted by different vectors, and cluster in distinctive genotypes. Recently isolated strains from VL and HIV-VL co-infected patients in nort...
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ftdoajarticles:oai:doaj.org/article:86a5fea10ad54a998d41f801484688f3 2023-05-15T15:14:43+02:00 Polymorphism in the HASPB repeat region of East African Leishmania donovani strains. Arie Zackay Abdelmajeed Nasereddin Yegnasew Takele Dagimawie Tadesse Workagegnehu Hailu Zewdu Hurissa Sisay Yifru Teklu Weldegebreal Ermias Diro Aysheshm Kassahun Asrat Hailu Charles L Jaffe 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002031 https://doaj.org/article/86a5fea10ad54a998d41f801484688f3 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3554577?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002031 https://doaj.org/article/86a5fea10ad54a998d41f801484688f3 PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e2031 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002031 2022-12-31T15:58:41Z BACKGROUND/OBJECTIVES: Visceral leishmaniasis (VL) caused by Leishmania donovani is a major health problem in Ethiopia. Parasites in disparate regions are transmitted by different vectors, and cluster in distinctive genotypes. Recently isolated strains from VL and HIV-VL co-infected patients in north and south Ethiopia were characterized as part of a longitudinal study on VL transmission. METHODOLOGY/PRINCIPAL FINDINGS: Sixty-three L. donovani strains were examined by polymerase chain reaction (PCR) targeting three regions: internal transcribed spacer 1 (ITS1), cysteine protease B (cpb), and HASPB (k26). ITS1- and cpb--PCR identified these strains as L. donovani. Interestingly, the k26--PCR amplicon size varied depending on the patient's geographic origin. Most strains from northwestern Ethiopia (36/40) produced a 290 bp product with a minority (4/40) giving a 410 bp amplicon. All of the latter strains were isolated from patients with HIV-VL co-infections, while the former group contained both VL and HIV-VL co-infected patients. Almost all the strains (20/23) from southwestern Ethiopia produced a 450 bp amplicon with smaller products (290 or 360 bp) only observed for three strains. Sudanese strains produced amplicons identical (290 bp) to those found in northwestern Ethiopia; while Kenyan strains gave larger PCR products (500 and 650 bp). High-resolution melt (HRM) analysis distinguished the different PCR products. Sequence analysis showed that the k26 repeat region in L. donovani is comprised of polymorphic 13 and 14 amino acid motifs. The 13 amino acid peptide motifs, prevalent in L. donovani, are rare in L. infantum. The number and order of the repeats in L. donovani varies between geographic regions. CONCLUSIONS/SIGNIFICANCE: HASPB repeat region (k26) shows considerable polymorphism among L. donovani strains from different regions in East Africa. This should be taken into account when designing diagnostic assays and vaccines based on this antigen. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 1 e2031 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Arie Zackay Abdelmajeed Nasereddin Yegnasew Takele Dagimawie Tadesse Workagegnehu Hailu Zewdu Hurissa Sisay Yifru Teklu Weldegebreal Ermias Diro Aysheshm Kassahun Asrat Hailu Charles L Jaffe Polymorphism in the HASPB repeat region of East African Leishmania donovani strains. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND/OBJECTIVES: Visceral leishmaniasis (VL) caused by Leishmania donovani is a major health problem in Ethiopia. Parasites in disparate regions are transmitted by different vectors, and cluster in distinctive genotypes. Recently isolated strains from VL and HIV-VL co-infected patients in north and south Ethiopia were characterized as part of a longitudinal study on VL transmission. METHODOLOGY/PRINCIPAL FINDINGS: Sixty-three L. donovani strains were examined by polymerase chain reaction (PCR) targeting three regions: internal transcribed spacer 1 (ITS1), cysteine protease B (cpb), and HASPB (k26). ITS1- and cpb--PCR identified these strains as L. donovani. Interestingly, the k26--PCR amplicon size varied depending on the patient's geographic origin. Most strains from northwestern Ethiopia (36/40) produced a 290 bp product with a minority (4/40) giving a 410 bp amplicon. All of the latter strains were isolated from patients with HIV-VL co-infections, while the former group contained both VL and HIV-VL co-infected patients. Almost all the strains (20/23) from southwestern Ethiopia produced a 450 bp amplicon with smaller products (290 or 360 bp) only observed for three strains. Sudanese strains produced amplicons identical (290 bp) to those found in northwestern Ethiopia; while Kenyan strains gave larger PCR products (500 and 650 bp). High-resolution melt (HRM) analysis distinguished the different PCR products. Sequence analysis showed that the k26 repeat region in L. donovani is comprised of polymorphic 13 and 14 amino acid motifs. The 13 amino acid peptide motifs, prevalent in L. donovani, are rare in L. infantum. The number and order of the repeats in L. donovani varies between geographic regions. CONCLUSIONS/SIGNIFICANCE: HASPB repeat region (k26) shows considerable polymorphism among L. donovani strains from different regions in East Africa. This should be taken into account when designing diagnostic assays and vaccines based on this antigen. |
format |
Article in Journal/Newspaper |
author |
Arie Zackay Abdelmajeed Nasereddin Yegnasew Takele Dagimawie Tadesse Workagegnehu Hailu Zewdu Hurissa Sisay Yifru Teklu Weldegebreal Ermias Diro Aysheshm Kassahun Asrat Hailu Charles L Jaffe |
author_facet |
Arie Zackay Abdelmajeed Nasereddin Yegnasew Takele Dagimawie Tadesse Workagegnehu Hailu Zewdu Hurissa Sisay Yifru Teklu Weldegebreal Ermias Diro Aysheshm Kassahun Asrat Hailu Charles L Jaffe |
author_sort |
Arie Zackay |
title |
Polymorphism in the HASPB repeat region of East African Leishmania donovani strains. |
title_short |
Polymorphism in the HASPB repeat region of East African Leishmania donovani strains. |
title_full |
Polymorphism in the HASPB repeat region of East African Leishmania donovani strains. |
title_fullStr |
Polymorphism in the HASPB repeat region of East African Leishmania donovani strains. |
title_full_unstemmed |
Polymorphism in the HASPB repeat region of East African Leishmania donovani strains. |
title_sort |
polymorphism in the haspb repeat region of east african leishmania donovani strains. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002031 https://doaj.org/article/86a5fea10ad54a998d41f801484688f3 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 1, p e2031 (2013) |
op_relation |
http://europepmc.org/articles/PMC3554577?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002031 https://doaj.org/article/86a5fea10ad54a998d41f801484688f3 |
op_doi |
https://doi.org/10.1371/journal.pntd.0002031 |
container_title |
PLoS Neglected Tropical Diseases |
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7 |
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1 |
container_start_page |
e2031 |
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1766345145201983488 |