Immobilization studies of Candida Antarctica lipase B on gallic acid resin-grafted magnetic iron oxide nanoparticles

• Main Authors: SreeHarsha N, Ghorpade RV, Alzahrani AM, Al-Dhubiab BE, Venugopala KN
• Format: Article in Journal/Newspaper
• Language: English
• Published: Dove Medical Press 2019
• Subjects: Nanoparticles; Candida Antarctica Lipase B; grafting; immobilization; Medicine (General); R5-920; Antarc*; Antarctica
• Online Access: https://doaj.org/article/8656e96c9333486092f09de4a536a429

Nagaraja SreeHarsha,1 Ravindra V Ghorpade,2 Abdullah Mossa Alzahrani,3 Bandar E Al-Dhubiab,1 Katharigatta N Venugopala1,41Department of Pharmaceutical Sciences, College of Clinical Pharmacy, King Faisal University, Al-Ahsa, Saudi Arabia; 2Chemical Engineering and Process Development Division, CSIR-National Chemical Laboratory, Pune 411 008, India; 3Department of Biological Sciences, College of Science, King Faisal University, Al-Ahsa, Saudi Arabia; 4Department of Biotechnology and Food Technology, Durban University of Technology, Durban 4001, South AfricaPurpose: Here, we present the successful preparation of a highly efficient gallic acid resin grafted with magnetic nanoparticles (MNPs) and containing a branched brush polymeric shell.Methods: Using a convenient co-precipitation method, we prepared Fe3O4 nanoparticles stabilized by citric acid. These nanoparticles underwent further silica modification and amino functionalization followed by gallic acid functionalization on their surface. Under alkaline conditions, we used a condensation reaction that combined formaldehyde and gallic, to graft the gallic acid−formaldehyde resin on the surface. We then evaluated the polymer-grafted MNPs to assay the Candida Antarctica B lipase(Cal-B) immobilization via physical adsorption.Conclusion: Furthermore, during optimization of parameters that defined conditions of immobilization, we found that the optimum immobilization was achieved in 15 mins. Also, optimal immobilization temperature and pH were 38ºC and 7.5, respectively. In addition, the reusability study of immobilized lipase polymer-grafted MNPs was done by isolating the MNPs from the reaction medium using magnetic separation, which showed that grafted MNPs reached 5 cycles with 91% activity retention.Keywords: nanoparticles, Candida Antarctica lipase B, grafting, immobilization