Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran)
Objective: To molecularly identify Nosema species in provinces of Isfahan, Fars, Chaharmahal and Bakhtiari. Methods: One hundred and eighty adult honey bees suspected with nosemosis from provinces of Fars (different counties), Isfahan, and Chaharmahal and Bakhtiari were tested. In order to determine...
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Wolters Kluwer Medknow Publications
2017
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ftdoajarticles:oai:doaj.org/article:8634e508bc814651b1f78184f7d0bf32 2023-05-15T15:02:38+02:00 Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran) Fatemeh Aroee Hamidreza Azizi Behrouz Shiran Khodadad Pirali Kheirabadi 2017-01-01T00:00:00Z https://doi.org/10.1016/j.apjtb.2016.11.004 https://doaj.org/article/8634e508bc814651b1f78184f7d0bf32 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S222116911630082X https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.11.004 https://doaj.org/article/8634e508bc814651b1f78184f7d0bf32 Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 1, Pp 10-13 (2017) Microsporidia Nosema apis Nosema ceranae PCR Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2017 ftdoajarticles https://doi.org/10.1016/j.apjtb.2016.11.004 2022-12-31T04:43:52Z Objective: To molecularly identify Nosema species in provinces of Isfahan, Fars, Chaharmahal and Bakhtiari. Methods: One hundred and eighty adult honey bees suspected with nosemosis from provinces of Fars (different counties), Isfahan, and Chaharmahal and Bakhtiari were tested. In order to determine the species of Nosema, previously developed PCR and primers based on 16S rRNA gene were used. PCR products were purified and sent to the Korean company of Macrogen for sequencing. Results: Only Nosema ceranae was determined in all samples based on their molecular profile. Sequences of the 16S rRNA gene were sent to GenBank/NCBI (samples accession numbers KP318660–KP318663). Conclusions: This species currently exists in European honeybee apiaries of Apis mellifera in the studied provinces. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 7 1 10 13 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Microsporidia Nosema apis Nosema ceranae PCR Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Microsporidia Nosema apis Nosema ceranae PCR Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Fatemeh Aroee Hamidreza Azizi Behrouz Shiran Khodadad Pirali Kheirabadi Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran) |
topic_facet |
Microsporidia Nosema apis Nosema ceranae PCR Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To molecularly identify Nosema species in provinces of Isfahan, Fars, Chaharmahal and Bakhtiari. Methods: One hundred and eighty adult honey bees suspected with nosemosis from provinces of Fars (different counties), Isfahan, and Chaharmahal and Bakhtiari were tested. In order to determine the species of Nosema, previously developed PCR and primers based on 16S rRNA gene were used. PCR products were purified and sent to the Korean company of Macrogen for sequencing. Results: Only Nosema ceranae was determined in all samples based on their molecular profile. Sequences of the 16S rRNA gene were sent to GenBank/NCBI (samples accession numbers KP318660–KP318663). Conclusions: This species currently exists in European honeybee apiaries of Apis mellifera in the studied provinces. |
format |
Article in Journal/Newspaper |
author |
Fatemeh Aroee Hamidreza Azizi Behrouz Shiran Khodadad Pirali Kheirabadi |
author_facet |
Fatemeh Aroee Hamidreza Azizi Behrouz Shiran Khodadad Pirali Kheirabadi |
author_sort |
Fatemeh Aroee |
title |
Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran) |
title_short |
Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran) |
title_full |
Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran) |
title_fullStr |
Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran) |
title_full_unstemmed |
Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran) |
title_sort |
molecular identification of nosema species in provinces of fars, chaharmahal and bakhtiari and isfahan (southwestern iran) |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2017 |
url |
https://doi.org/10.1016/j.apjtb.2016.11.004 https://doaj.org/article/8634e508bc814651b1f78184f7d0bf32 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 7, Iss 1, Pp 10-13 (2017) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S222116911630082X https://doaj.org/toc/2221-1691 2221-1691 doi:10.1016/j.apjtb.2016.11.004 https://doaj.org/article/8634e508bc814651b1f78184f7d0bf32 |
op_doi |
https://doi.org/10.1016/j.apjtb.2016.11.004 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
7 |
container_issue |
1 |
container_start_page |
10 |
op_container_end_page |
13 |
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1766334565309218816 |