Molecular identification of Nosema species in provinces of Fars, Chaharmahal and Bakhtiari and Isfahan (Southwestern Iran)

Objective: To molecularly identify Nosema species in provinces of Isfahan, Fars, Chaharmahal and Bakhtiari. Methods: One hundred and eighty adult honey bees suspected with nosemosis from provinces of Fars (different counties), Isfahan, and Chaharmahal and Bakhtiari were tested. In order to determine...

Full description

Bibliographic Details
Published in:Asian Pacific Journal of Tropical Biomedicine
Main Authors: Fatemeh Aroee, Hamidreza Azizi, Behrouz Shiran, Khodadad Pirali Kheirabadi
Format: Article in Journal/Newspaper
Language:English
Published: Wolters Kluwer Medknow Publications 2017
Subjects:
Microsporidia
Nosema apis
Nosema ceranae
PCR
Arctic medicine. Tropical medicine
RC955-962
Biology (General)
QH301-705.5
Arctic
Online Access:https://doi.org/10.1016/j.apjtb.2016.11.004
https://doaj.org/article/8634e508bc814651b1f78184f7d0bf32
Description
Summary:Objective: To molecularly identify Nosema species in provinces of Isfahan, Fars, Chaharmahal and Bakhtiari. Methods: One hundred and eighty adult honey bees suspected with nosemosis from provinces of Fars (different counties), Isfahan, and Chaharmahal and Bakhtiari were tested. In order to determine the species of Nosema, previously developed PCR and primers based on 16S rRNA gene were used. PCR products were purified and sent to the Korean company of Macrogen for sequencing. Results: Only Nosema ceranae was determined in all samples based on their molecular profile. Sequences of the 16S rRNA gene were sent to GenBank/NCBI (samples accession numbers KP318660–KP318663). Conclusions: This species currently exists in European honeybee apiaries of Apis mellifera in the studied provinces.

Similar Items