Measles serodiagnosis: standardization and evaluation of a dot-ELISA Diagnóstico sorológico do sarampo: padronização e avaliação do teste Dot-ELISA
A Dot-ELISA using a measles virus (MV) antigen obtained by sodium deoxycholate treatment was standardized and evaluated for IgM and IgG antibody detection in measles patients and measles-vaccinated subjects. A total of 192 serum samples were studied, comprising 47 from patients with acute and conval...
Published in: | Revista do Instituto de Medicina Tropical de São Paulo |
---|---|
Main Authors: | , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Universidade de São Paulo (USP)
1994
|
Subjects: | |
Online Access: | https://doi.org/10.1590/S0036-46651994000200008 https://doaj.org/article/8614e6d97c7346938d6b18ef8f86466d |
id |
ftdoajarticles:oai:doaj.org/article:8614e6d97c7346938d6b18ef8f86466d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:8614e6d97c7346938d6b18ef8f86466d 2024-09-09T19:28:27+00:00 Measles serodiagnosis: standardization and evaluation of a dot-ELISA Diagnóstico sorológico do sarampo: padronização e avaliação do teste Dot-ELISA Lourdes R. A. Vaz de Lima Sumie Hoshino-Shimizu Vanda A. U. F. de Souza Claudio S. Pannuti Heitor F. Andrade Junior Laura M. Sumita Antonio W. Ferreira 1994-04-01T00:00:00Z https://doi.org/10.1590/S0036-46651994000200008 https://doaj.org/article/8614e6d97c7346938d6b18ef8f86466d EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651994000200008 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651994000200008 0036-4665 1678-9946 https://doaj.org/article/8614e6d97c7346938d6b18ef8f86466d Revista do Instituto de Medicina Tropical de São Paulo, Vol 36, Iss 2, Pp 139-147 (1994) Dot-ELISA Measles Serologic diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1994 ftdoajarticles https://doi.org/10.1590/S0036-46651994000200008 2024-08-05T17:49:30Z A Dot-ELISA using a measles virus (MV) antigen obtained by sodium deoxycholate treatment was standardized and evaluated for IgM and IgG antibody detection in measles patients and measles-vaccinated subjects. A total of 192 serum samples were studied, comprising 47 from patients with acute and convalescent measles, 55 from 9-month old children prior to measles vaccination and 41 from children of the same age after vaccination, and 49 from patients with unrelated diseases. The diagnostic performances of the IgG Dot-ELISA and IgG immuno fluorescence test (IFT) were found to be close, varying from 0.97 to 1.00 in sensitivity and the specificities were maximum (1.00). Nevertheless, the sensitivity of the IgM Dot-ELISA (0.85) was higher than that (0.63) of the IgM IFT, although both assays had comparably high (1.00) specificities. The IgM Dot-ELISA in particular proved to be more sensitive in relation to other assays studied by revealing antibodies in 80.0% (12/15) of vaccinated children on the 15th day after immunization. In contrast the IgM IFT, failed to detect antibodies in the same group of vaccinated children. The stability of the MV antigen was longer than that of the IFT antigen, and the reproducibility of the Dot-Elisa was satisfactory. A técnica de Dot-ELISA (DE) para detecção de anticorpos IgM e IgG anti vírus do sarampo foi padronizada e avaliada utilizando-se antígeno viral obtido por tratamento com desoxicolato de sódio (DOC). Foram estudadas 192 amostras de soros, compreendendo 47 amostras de 22 pacientes com sarampo nas fases aguda e convalescente, 55 amostras de soros de crianças antes da vacinação, tendo 9 meses de idade, 41 amostras de soros de crianças da mesma idade colhidas após vacinação e 49 amostras de soros de pacientes com outras patologias. O desempenho diagnóstico da técnica de Dot-ELISA-IgG foi semelhante ao de Imunofluorescência indireta (IFI) IgG cujos índices de sensibilidade variaram de 0,97 a 1,00 e os de especificidade sendo de valor máximo, 1,00. Contudo, a sensibilidade da técnica ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Aguda ENVELOPE(-26.750,-26.750,-76.150,-76.150) Arctic Revista do Instituto de Medicina Tropical de São Paulo 36 2 139 147 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Dot-ELISA Measles Serologic diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Dot-ELISA Measles Serologic diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Lourdes R. A. Vaz de Lima Sumie Hoshino-Shimizu Vanda A. U. F. de Souza Claudio S. Pannuti Heitor F. Andrade Junior Laura M. Sumita Antonio W. Ferreira Measles serodiagnosis: standardization and evaluation of a dot-ELISA Diagnóstico sorológico do sarampo: padronização e avaliação do teste Dot-ELISA |
topic_facet |
Dot-ELISA Measles Serologic diagnosis Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
A Dot-ELISA using a measles virus (MV) antigen obtained by sodium deoxycholate treatment was standardized and evaluated for IgM and IgG antibody detection in measles patients and measles-vaccinated subjects. A total of 192 serum samples were studied, comprising 47 from patients with acute and convalescent measles, 55 from 9-month old children prior to measles vaccination and 41 from children of the same age after vaccination, and 49 from patients with unrelated diseases. The diagnostic performances of the IgG Dot-ELISA and IgG immuno fluorescence test (IFT) were found to be close, varying from 0.97 to 1.00 in sensitivity and the specificities were maximum (1.00). Nevertheless, the sensitivity of the IgM Dot-ELISA (0.85) was higher than that (0.63) of the IgM IFT, although both assays had comparably high (1.00) specificities. The IgM Dot-ELISA in particular proved to be more sensitive in relation to other assays studied by revealing antibodies in 80.0% (12/15) of vaccinated children on the 15th day after immunization. In contrast the IgM IFT, failed to detect antibodies in the same group of vaccinated children. The stability of the MV antigen was longer than that of the IFT antigen, and the reproducibility of the Dot-Elisa was satisfactory. A técnica de Dot-ELISA (DE) para detecção de anticorpos IgM e IgG anti vírus do sarampo foi padronizada e avaliada utilizando-se antígeno viral obtido por tratamento com desoxicolato de sódio (DOC). Foram estudadas 192 amostras de soros, compreendendo 47 amostras de 22 pacientes com sarampo nas fases aguda e convalescente, 55 amostras de soros de crianças antes da vacinação, tendo 9 meses de idade, 41 amostras de soros de crianças da mesma idade colhidas após vacinação e 49 amostras de soros de pacientes com outras patologias. O desempenho diagnóstico da técnica de Dot-ELISA-IgG foi semelhante ao de Imunofluorescência indireta (IFI) IgG cujos índices de sensibilidade variaram de 0,97 a 1,00 e os de especificidade sendo de valor máximo, 1,00. Contudo, a sensibilidade da técnica ... |
format |
Article in Journal/Newspaper |
author |
Lourdes R. A. Vaz de Lima Sumie Hoshino-Shimizu Vanda A. U. F. de Souza Claudio S. Pannuti Heitor F. Andrade Junior Laura M. Sumita Antonio W. Ferreira |
author_facet |
Lourdes R. A. Vaz de Lima Sumie Hoshino-Shimizu Vanda A. U. F. de Souza Claudio S. Pannuti Heitor F. Andrade Junior Laura M. Sumita Antonio W. Ferreira |
author_sort |
Lourdes R. A. Vaz de Lima |
title |
Measles serodiagnosis: standardization and evaluation of a dot-ELISA Diagnóstico sorológico do sarampo: padronização e avaliação do teste Dot-ELISA |
title_short |
Measles serodiagnosis: standardization and evaluation of a dot-ELISA Diagnóstico sorológico do sarampo: padronização e avaliação do teste Dot-ELISA |
title_full |
Measles serodiagnosis: standardization and evaluation of a dot-ELISA Diagnóstico sorológico do sarampo: padronização e avaliação do teste Dot-ELISA |
title_fullStr |
Measles serodiagnosis: standardization and evaluation of a dot-ELISA Diagnóstico sorológico do sarampo: padronização e avaliação do teste Dot-ELISA |
title_full_unstemmed |
Measles serodiagnosis: standardization and evaluation of a dot-ELISA Diagnóstico sorológico do sarampo: padronização e avaliação do teste Dot-ELISA |
title_sort |
measles serodiagnosis: standardization and evaluation of a dot-elisa diagnóstico sorológico do sarampo: padronização e avaliação do teste dot-elisa |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1994 |
url |
https://doi.org/10.1590/S0036-46651994000200008 https://doaj.org/article/8614e6d97c7346938d6b18ef8f86466d |
long_lat |
ENVELOPE(-26.750,-26.750,-76.150,-76.150) |
geographic |
Aguda Arctic |
geographic_facet |
Aguda Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 36, Iss 2, Pp 139-147 (1994) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651994000200008 https://doaj.org/toc/0036-4665 https://doaj.org/toc/1678-9946 doi:10.1590/S0036-46651994000200008 0036-4665 1678-9946 https://doaj.org/article/8614e6d97c7346938d6b18ef8f86466d |
op_doi |
https://doi.org/10.1590/S0036-46651994000200008 |
container_title |
Revista do Instituto de Medicina Tropical de São Paulo |
container_volume |
36 |
container_issue |
2 |
container_start_page |
139 |
op_container_end_page |
147 |
_version_ |
1809897775433777152 |