Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel
In spite of many decades of research, the spawning migration of the European eel Anguilla anguilla from the European coast to the Sargasso Sea remains a mystery. In particular, the role of the swimbladder as a buoyancy regulating structure is not yet understood. In this study, we exercised silver ee...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Public Library of Science (PLoS)
2020
|
Subjects: | |
Online Access: | https://doaj.org/article/85e09654f2a547cd8099633b17e065e4 |
id |
ftdoajarticles:oai:doaj.org/article:85e09654f2a547cd8099633b17e065e4 |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:85e09654f2a547cd8099633b17e065e4 2023-05-15T13:28:16+02:00 Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel Gabriel Schneebauer Constantin Lindemann Victoria Drechsel Lasse Marohn Klaus Wysujack Elena Santidrian Ron Dirks Reinhold Hanel Bernd Pelster Nicholas Simon Foulkes 2020-01-01T00:00:00Z https://doaj.org/article/85e09654f2a547cd8099633b17e065e4 EN eng Public Library of Science (PLoS) https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526912/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 https://doaj.org/article/85e09654f2a547cd8099633b17e065e4 PLoS ONE, Vol 15, Iss 9 (2020) Medicine R Science Q article 2020 ftdoajarticles 2022-12-31T11:18:38Z In spite of many decades of research, the spawning migration of the European eel Anguilla anguilla from the European coast to the Sargasso Sea remains a mystery. In particular, the role of the swimbladder as a buoyancy regulating structure is not yet understood. In this study, we exercised silver eels in a swim tunnel under elevated hydrostatic pressure. The transcriptome of gas gland tissue of these exercised eels was then compared to the known transcriptome of not exercised (control) silver eel gas gland cells. Due to the high infection rate of the eel population with the swimbladder parasite Anguillicola crassus, the comparison also included an exercised group of silver eels with a heavily damaged swimbladder, and we compared the previously published transcriptome of not exercised silver eels with a highly damaged swimbladder with the exercised group of silver eels with a heavily damaged swimbladder. The comparisons of unexercised (control) silver eels with exercised silver eels with functional swimbladder (EF), as well as with exercised silver eels with damaged swimbladder (ED), both showed a significant elevation in transcripts related to glycolytic enzymes. This could also be observed within the comparison of unexercised silver eels with a highly infected swimbladder with exercised eels with a damaged swimbladder (DED). In contrast to EF, in ED a significant elevation in transcript numbers of mitochondrial NADH dehydrogenase was observed. While in EF the transcriptional changes suggested that acid production and secretion was enhanced, in ED these changes appeared to be related to thickened tissue and thus elevated diffusion distances. The remarkable number of differentially expressed transcripts coding for proteins connected to cAMP-dependent signaling pathways indicated that metabolic control in gas gland cells includes cAMP-dependent pathways. In contrast to ED, in EF significant transcriptional changes could be related to the reconstruction of the extracellular matrix, while in ED tissue repair and ... Article in Journal/Newspaper Anguilla anguilla Directory of Open Access Journals: DOAJ Articles |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Gabriel Schneebauer Constantin Lindemann Victoria Drechsel Lasse Marohn Klaus Wysujack Elena Santidrian Ron Dirks Reinhold Hanel Bernd Pelster Nicholas Simon Foulkes Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel |
topic_facet |
Medicine R Science Q |
description |
In spite of many decades of research, the spawning migration of the European eel Anguilla anguilla from the European coast to the Sargasso Sea remains a mystery. In particular, the role of the swimbladder as a buoyancy regulating structure is not yet understood. In this study, we exercised silver eels in a swim tunnel under elevated hydrostatic pressure. The transcriptome of gas gland tissue of these exercised eels was then compared to the known transcriptome of not exercised (control) silver eel gas gland cells. Due to the high infection rate of the eel population with the swimbladder parasite Anguillicola crassus, the comparison also included an exercised group of silver eels with a heavily damaged swimbladder, and we compared the previously published transcriptome of not exercised silver eels with a highly damaged swimbladder with the exercised group of silver eels with a heavily damaged swimbladder. The comparisons of unexercised (control) silver eels with exercised silver eels with functional swimbladder (EF), as well as with exercised silver eels with damaged swimbladder (ED), both showed a significant elevation in transcripts related to glycolytic enzymes. This could also be observed within the comparison of unexercised silver eels with a highly infected swimbladder with exercised eels with a damaged swimbladder (DED). In contrast to EF, in ED a significant elevation in transcript numbers of mitochondrial NADH dehydrogenase was observed. While in EF the transcriptional changes suggested that acid production and secretion was enhanced, in ED these changes appeared to be related to thickened tissue and thus elevated diffusion distances. The remarkable number of differentially expressed transcripts coding for proteins connected to cAMP-dependent signaling pathways indicated that metabolic control in gas gland cells includes cAMP-dependent pathways. In contrast to ED, in EF significant transcriptional changes could be related to the reconstruction of the extracellular matrix, while in ED tissue repair and ... |
format |
Article in Journal/Newspaper |
author |
Gabriel Schneebauer Constantin Lindemann Victoria Drechsel Lasse Marohn Klaus Wysujack Elena Santidrian Ron Dirks Reinhold Hanel Bernd Pelster Nicholas Simon Foulkes |
author_facet |
Gabriel Schneebauer Constantin Lindemann Victoria Drechsel Lasse Marohn Klaus Wysujack Elena Santidrian Ron Dirks Reinhold Hanel Bernd Pelster Nicholas Simon Foulkes |
author_sort |
Gabriel Schneebauer |
title |
Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel |
title_short |
Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel |
title_full |
Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel |
title_fullStr |
Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel |
title_full_unstemmed |
Swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the European eel |
title_sort |
swimming under elevated hydrostatic pressure increases glycolytic activity in gas gland cells of the european eel |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doaj.org/article/85e09654f2a547cd8099633b17e065e4 |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_source |
PLoS ONE, Vol 15, Iss 9 (2020) |
op_relation |
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7526912/?tool=EBI https://doaj.org/toc/1932-6203 1932-6203 https://doaj.org/article/85e09654f2a547cd8099633b17e065e4 |
_version_ |
1766403093866479616 |