Phytoplankton Productivity in an Arctic Fjord (West Greenland): Estimating Electron Requirements for Carbon Fixation and Oxygen Production.
Accurate quantification of pelagic primary production is essential for quantifying the marine carbon turnover and the energy supply to the food web. Knowing the electron requirement (Κ) for carbon (C) fixation (ΚC) and oxygen (O2) production (ΚO2), variable fluorescence has the potential to quantify...
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ftdoajarticles:oai:doaj.org/article:85c4a371526e4376b161cb8933149be3 2023-05-15T15:00:40+02:00 Phytoplankton Productivity in an Arctic Fjord (West Greenland): Estimating Electron Requirements for Carbon Fixation and Oxygen Production. Kasper Hancke Tage Dalsgaard Mikael Kristian Sejr Stiig Markager Ronnie Nøhr Glud 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0133275 https://doaj.org/article/85c4a371526e4376b161cb8933149be3 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4517866?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0133275 https://doaj.org/article/85c4a371526e4376b161cb8933149be3 PLoS ONE, Vol 10, Iss 7, p e0133275 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0133275 2022-12-31T06:37:45Z Accurate quantification of pelagic primary production is essential for quantifying the marine carbon turnover and the energy supply to the food web. Knowing the electron requirement (Κ) for carbon (C) fixation (ΚC) and oxygen (O2) production (ΚO2), variable fluorescence has the potential to quantify primary production in microalgae, and hereby increasing spatial and temporal resolution of measurements compared to traditional methods. Here we quantify ΚC and ΚO2 through measures of Pulse Amplitude Modulated (PAM) fluorometry, C fixation and O2 production in an Arctic fjord (Godthåbsfjorden, W Greenland). Through short- (2h) and long-term (24h) experiments, rates of electron transfer (ETRPSII), C fixation and/or O2 production were quantified and compared. Absolute rates of ETR were derived by accounting for Photosystem II light absorption and spectral light composition. Two-hour incubations revealed a linear relationship between ETRPSII and gross 14C fixation (R2 = 0.81) during light-limited photosynthesis, giving a ΚC of 7.6 ± 0.6 (mean ± S.E.) mol é (mol C)-1. Diel net rates also demonstrated a linear relationship between ETRPSII and C fixation giving a ΚC of 11.2 ± 1.3 mol é (mol C)-1 (R2 = 0.86). For net O2 production the electron requirement was lower than for net C fixation giving 6.5 ± 0.9 mol é (mol O2)-1 (R2 = 0.94). This, however, still is an electron requirement 1.6 times higher than the theoretical minimum for O2 production [i.e. 4 mol é (mol O2)-1]. The discrepancy is explained by respiratory activity and non-photochemical electron requirements and the variability is discussed. In conclusion, the bio-optical method and derived electron requirement support conversion of ETR to units of C or O2, paving the road for improved spatial and temporal resolution of primary production estimates. Article in Journal/Newspaper Arctic Greenland Phytoplankton Directory of Open Access Journals: DOAJ Articles Arctic Greenland PLOS ONE 10 7 e0133275 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Medicine R Science Q |
spellingShingle |
Medicine R Science Q Kasper Hancke Tage Dalsgaard Mikael Kristian Sejr Stiig Markager Ronnie Nøhr Glud Phytoplankton Productivity in an Arctic Fjord (West Greenland): Estimating Electron Requirements for Carbon Fixation and Oxygen Production. |
topic_facet |
Medicine R Science Q |
description |
Accurate quantification of pelagic primary production is essential for quantifying the marine carbon turnover and the energy supply to the food web. Knowing the electron requirement (Κ) for carbon (C) fixation (ΚC) and oxygen (O2) production (ΚO2), variable fluorescence has the potential to quantify primary production in microalgae, and hereby increasing spatial and temporal resolution of measurements compared to traditional methods. Here we quantify ΚC and ΚO2 through measures of Pulse Amplitude Modulated (PAM) fluorometry, C fixation and O2 production in an Arctic fjord (Godthåbsfjorden, W Greenland). Through short- (2h) and long-term (24h) experiments, rates of electron transfer (ETRPSII), C fixation and/or O2 production were quantified and compared. Absolute rates of ETR were derived by accounting for Photosystem II light absorption and spectral light composition. Two-hour incubations revealed a linear relationship between ETRPSII and gross 14C fixation (R2 = 0.81) during light-limited photosynthesis, giving a ΚC of 7.6 ± 0.6 (mean ± S.E.) mol é (mol C)-1. Diel net rates also demonstrated a linear relationship between ETRPSII and C fixation giving a ΚC of 11.2 ± 1.3 mol é (mol C)-1 (R2 = 0.86). For net O2 production the electron requirement was lower than for net C fixation giving 6.5 ± 0.9 mol é (mol O2)-1 (R2 = 0.94). This, however, still is an electron requirement 1.6 times higher than the theoretical minimum for O2 production [i.e. 4 mol é (mol O2)-1]. The discrepancy is explained by respiratory activity and non-photochemical electron requirements and the variability is discussed. In conclusion, the bio-optical method and derived electron requirement support conversion of ETR to units of C or O2, paving the road for improved spatial and temporal resolution of primary production estimates. |
format |
Article in Journal/Newspaper |
author |
Kasper Hancke Tage Dalsgaard Mikael Kristian Sejr Stiig Markager Ronnie Nøhr Glud |
author_facet |
Kasper Hancke Tage Dalsgaard Mikael Kristian Sejr Stiig Markager Ronnie Nøhr Glud |
author_sort |
Kasper Hancke |
title |
Phytoplankton Productivity in an Arctic Fjord (West Greenland): Estimating Electron Requirements for Carbon Fixation and Oxygen Production. |
title_short |
Phytoplankton Productivity in an Arctic Fjord (West Greenland): Estimating Electron Requirements for Carbon Fixation and Oxygen Production. |
title_full |
Phytoplankton Productivity in an Arctic Fjord (West Greenland): Estimating Electron Requirements for Carbon Fixation and Oxygen Production. |
title_fullStr |
Phytoplankton Productivity in an Arctic Fjord (West Greenland): Estimating Electron Requirements for Carbon Fixation and Oxygen Production. |
title_full_unstemmed |
Phytoplankton Productivity in an Arctic Fjord (West Greenland): Estimating Electron Requirements for Carbon Fixation and Oxygen Production. |
title_sort |
phytoplankton productivity in an arctic fjord (west greenland): estimating electron requirements for carbon fixation and oxygen production. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0133275 https://doaj.org/article/85c4a371526e4376b161cb8933149be3 |
geographic |
Arctic Greenland |
geographic_facet |
Arctic Greenland |
genre |
Arctic Greenland Phytoplankton |
genre_facet |
Arctic Greenland Phytoplankton |
op_source |
PLoS ONE, Vol 10, Iss 7, p e0133275 (2015) |
op_relation |
http://europepmc.org/articles/PMC4517866?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0133275 https://doaj.org/article/85c4a371526e4376b161cb8933149be3 |
op_doi |
https://doi.org/10.1371/journal.pone.0133275 |
container_title |
PLOS ONE |
container_volume |
10 |
container_issue |
7 |
container_start_page |
e0133275 |
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