An integrated in vitro imaging platform for characterizing filarial parasite behavior within a multicellular microenvironment.
Lymphatic Filariasis, a Neglected Tropical Disease, is caused by thread-like parasitic worms, including B. malayi, which migrate to the human lymphatic system following transmission. The parasites reside in collecting lymphatic vessels and lymph nodes for years, often resulting in lymphedema, elepha...
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ftdoajarticles:oai:doaj.org/article:85b38a293a5240a3b956aa1c0c850eb4 2023-05-15T15:12:11+02:00 An integrated in vitro imaging platform for characterizing filarial parasite behavior within a multicellular microenvironment. Timothy Kassis Henry M Skelton Iris M Lu Andrew R Moorhead J Brandon Dixon 2014-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003305 https://doaj.org/article/85b38a293a5240a3b956aa1c0c850eb4 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4238983?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003305 https://doaj.org/article/85b38a293a5240a3b956aa1c0c850eb4 PLoS Neglected Tropical Diseases, Vol 8, Iss 11, p e3305 (2014) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2014 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003305 2022-12-31T14:13:26Z Lymphatic Filariasis, a Neglected Tropical Disease, is caused by thread-like parasitic worms, including B. malayi, which migrate to the human lymphatic system following transmission. The parasites reside in collecting lymphatic vessels and lymph nodes for years, often resulting in lymphedema, elephantiasis or hydrocele. The mechanisms driving worm migration and retention within the lymphatics are currently unknown. We have developed an integrated in vitro imaging platform capable of quantifying B. malayi migration and behavior in a multicellular microenvironment relevant to the initial site of worm injection by incorporating the worm in a Polydimethylsiloxane (PDMS) microchannel in the presence of human dermal lymphatic endothelial cells (LECs) and human dermal fibroblasts (HDFs). The platform utilizes a motorized controllable microscope with CO2 and temperature regulation to allow for worm tracking experiments with high resolution over large length and time scales. Using post-acquisition algorithms, we quantified four parameters: 1) speed, 2) thrashing intensity, 3) percentage of time spent in a given cell region and 4) persistence ratio. We demonstrated the utility of our system by quantifying these parameters for L3 B. malayi in the presence of LECs and HDFs. Speed and thrashing increased in the presence of both cell types and were altered within minutes upon exposure to the anthelmintic drug, tetramisole. The worms displayed no targeted migration towards either cell type for the time course of this study (3 hours). When cells were not present in the chamber, worm thrashing correlated directly with worm speed. However, this correlation was lost in the presence of cells. The described platform provides the ability to further study B. malayi migration and behavior. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 8 11 e3305 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Timothy Kassis Henry M Skelton Iris M Lu Andrew R Moorhead J Brandon Dixon An integrated in vitro imaging platform for characterizing filarial parasite behavior within a multicellular microenvironment. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Lymphatic Filariasis, a Neglected Tropical Disease, is caused by thread-like parasitic worms, including B. malayi, which migrate to the human lymphatic system following transmission. The parasites reside in collecting lymphatic vessels and lymph nodes for years, often resulting in lymphedema, elephantiasis or hydrocele. The mechanisms driving worm migration and retention within the lymphatics are currently unknown. We have developed an integrated in vitro imaging platform capable of quantifying B. malayi migration and behavior in a multicellular microenvironment relevant to the initial site of worm injection by incorporating the worm in a Polydimethylsiloxane (PDMS) microchannel in the presence of human dermal lymphatic endothelial cells (LECs) and human dermal fibroblasts (HDFs). The platform utilizes a motorized controllable microscope with CO2 and temperature regulation to allow for worm tracking experiments with high resolution over large length and time scales. Using post-acquisition algorithms, we quantified four parameters: 1) speed, 2) thrashing intensity, 3) percentage of time spent in a given cell region and 4) persistence ratio. We demonstrated the utility of our system by quantifying these parameters for L3 B. malayi in the presence of LECs and HDFs. Speed and thrashing increased in the presence of both cell types and were altered within minutes upon exposure to the anthelmintic drug, tetramisole. The worms displayed no targeted migration towards either cell type for the time course of this study (3 hours). When cells were not present in the chamber, worm thrashing correlated directly with worm speed. However, this correlation was lost in the presence of cells. The described platform provides the ability to further study B. malayi migration and behavior. |
format |
Article in Journal/Newspaper |
author |
Timothy Kassis Henry M Skelton Iris M Lu Andrew R Moorhead J Brandon Dixon |
author_facet |
Timothy Kassis Henry M Skelton Iris M Lu Andrew R Moorhead J Brandon Dixon |
author_sort |
Timothy Kassis |
title |
An integrated in vitro imaging platform for characterizing filarial parasite behavior within a multicellular microenvironment. |
title_short |
An integrated in vitro imaging platform for characterizing filarial parasite behavior within a multicellular microenvironment. |
title_full |
An integrated in vitro imaging platform for characterizing filarial parasite behavior within a multicellular microenvironment. |
title_fullStr |
An integrated in vitro imaging platform for characterizing filarial parasite behavior within a multicellular microenvironment. |
title_full_unstemmed |
An integrated in vitro imaging platform for characterizing filarial parasite behavior within a multicellular microenvironment. |
title_sort |
integrated in vitro imaging platform for characterizing filarial parasite behavior within a multicellular microenvironment. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pntd.0003305 https://doaj.org/article/85b38a293a5240a3b956aa1c0c850eb4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 8, Iss 11, p e3305 (2014) |
op_relation |
http://europepmc.org/articles/PMC4238983?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003305 https://doaj.org/article/85b38a293a5240a3b956aa1c0c850eb4 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003305 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
8 |
container_issue |
11 |
container_start_page |
e3305 |
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1766342910907777024 |