Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature
Abstract Background: Phoneutria nigriventer spider venom contains several cysteine-rich peptide toxins that act on different ion channels. Despite extensive studies on its venom and description of cDNA sequences of several of its toxin precursors, the gene structure of these toxins remains unknown....
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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ftdoajarticles:oai:doaj.org/article:85b0c37d432641d2b20d6540e7d9e1e4 2023-05-15T15:04:26+02:00 Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature Ana Luiza Bittencourt Paiva Alessandra Matavel Bruno César Souza Silva Clara Guerra-Duarte Marcelo Ribeiro Vasconcelos Diniz 2020-04-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2019-0075 https://doaj.org/article/85b0c37d432641d2b20d6540e7d9e1e4 EN eng SciELO http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20190075.pdf http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100801&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0075 https://doaj.org/article/85b0c37d432641d2b20d6540e7d9e1e4 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) Phoneutria Spider toxins Toxin genes Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2020 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2019-0075 2022-12-30T20:28:01Z Abstract Background: Phoneutria nigriventer spider venom contains several cysteine-rich peptide toxins that act on different ion channels. Despite extensive studies on its venom and description of cDNA sequences of several of its toxin precursors, the gene structure of these toxins remains unknown. Methods: Genomic regions encoding the precursors of three previously characterized P. nigriventer toxins - PnTx1, PnTx2-5 and PnTx4(5-5) - were amplified by PCR using specific primers. PCR fragments were cloned and sequenced. Obtained sequences were compared with their corresponding cDNA sequences. Results: The size of PCR fragments obtained and sequences corresponding to genomic regions encoding for the toxin precursors matched their cDNA sequences. Conclusions: Despite a few nucleotide substitutions in the genomic regions encoding for the toxin precursors when compared with cDNA sequences, the results of the present work indicate that P. nigriventer toxins do not contain introns in their genes sequences. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 26 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Phoneutria Spider toxins Toxin genes Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Phoneutria Spider toxins Toxin genes Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Ana Luiza Bittencourt Paiva Alessandra Matavel Bruno César Souza Silva Clara Guerra-Duarte Marcelo Ribeiro Vasconcelos Diniz Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature |
topic_facet |
Phoneutria Spider toxins Toxin genes Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract Background: Phoneutria nigriventer spider venom contains several cysteine-rich peptide toxins that act on different ion channels. Despite extensive studies on its venom and description of cDNA sequences of several of its toxin precursors, the gene structure of these toxins remains unknown. Methods: Genomic regions encoding the precursors of three previously characterized P. nigriventer toxins - PnTx1, PnTx2-5 and PnTx4(5-5) - were amplified by PCR using specific primers. PCR fragments were cloned and sequenced. Obtained sequences were compared with their corresponding cDNA sequences. Results: The size of PCR fragments obtained and sequences corresponding to genomic regions encoding for the toxin precursors matched their cDNA sequences. Conclusions: Despite a few nucleotide substitutions in the genomic regions encoding for the toxin precursors when compared with cDNA sequences, the results of the present work indicate that P. nigriventer toxins do not contain introns in their genes sequences. |
format |
Article in Journal/Newspaper |
author |
Ana Luiza Bittencourt Paiva Alessandra Matavel Bruno César Souza Silva Clara Guerra-Duarte Marcelo Ribeiro Vasconcelos Diniz |
author_facet |
Ana Luiza Bittencourt Paiva Alessandra Matavel Bruno César Souza Silva Clara Guerra-Duarte Marcelo Ribeiro Vasconcelos Diniz |
author_sort |
Ana Luiza Bittencourt Paiva |
title |
Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature |
title_short |
Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature |
title_full |
Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature |
title_fullStr |
Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature |
title_full_unstemmed |
Gene sequence analysis of toxins from the spider Phoneutria nigriventer revealed an intronless feature |
title_sort |
gene sequence analysis of toxins from the spider phoneutria nigriventer revealed an intronless feature |
publisher |
SciELO |
publishDate |
2020 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0075 https://doaj.org/article/85b0c37d432641d2b20d6540e7d9e1e4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 26 (2020) |
op_relation |
http://www.scielo.br/pdf/jvatitd/v26/1678-9199-jvatitd-26-e20190075.pdf http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100801&tlng=en https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2019-0075 https://doaj.org/article/85b0c37d432641d2b20d6540e7d9e1e4 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2019-0075 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
26 |
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